Label-free aptasensor for p24-HIV protein detection based on graphene quantum dots as an electrochemical signal amplifier

Gogola, Jeferson L.; Martins, Gustavo Celestino; Gevaerd, Ava; Blanes, Lucas; Cardoso, Josiane; Marchini, Fabrício Klerynton; Banks, Craig E.; Bergamini, Márcio F.; Marcolino‐Júnior, Luiz H.

doi:10.1016/j.aca.2021.338548

Cited by 37 publications

(17 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the former case, a range of geometries have been realized such as microbands, − dual-microbands, microband electrode arrays, microdisc arrays, microelectrodes and microelectrode arrays, and back-to-back configurations, highlighting the versatility of the SPE production methodology. SPEs are used extensively in the production of sensing platforms, with examples ranging from ions − and small molecules ,, to larger biological analytes such as proteins. − They have even been utilized outside of electrochemical sensing platforms due to their large surface area, ease of modification, disposability, and low cost. − As such, it is critical that SPEs can be reliably compared between different reported works.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Improvements Can Be Realized via Shortening the Length of Screen-Printed Electrochemical Platforms

et al. 2021

Self Cite

View full text Add to dashboard Cite

Screen-printed electrodes (SPEs) are ubiquitous within the field of electrochemistry and are commonplace within the arsenal of electrochemists. Their popularity stems from their reproducibility, versatility, and extremely lowcost production, allowing their utilization as single-shot electrodes and thus removing the need for tedious electrode pretreatments. Many SPE studies have explored changing the working electrode composition and/or size to benefit the researcher's specific applications. In this paper, we explore a critical parameter of SPEs that is often overlooked; namely, we explore changing the length of the SPE connections. We provide evidence of resistance changes through altering the connection length to the working electrode through theoretical calculations, multimeter measurements, and electrochemical impedance spectroscopy (EIS). We demonstrate that changing the physical length of SPE connections gives rise to more accurate heterogeneous electrode kinetics, which cannot be overcome simply through IR compensation. Significant improvements are observed when utilized as the basis of electrochemical sensing platforms for sodium nitrite, β-nicotinamide adenine dinucleotide (NADH), and lead (II). This work has a significant impact upon the field of SPEs and highlights the need for researchers to characterize and define their specific electrode performance. Without such fundamental characterization as the length and resistance of the SPE used, direct comparisons between two different systems for similar applications are obsolete. We therefore suggest that, when using SPEs in the future, experimentalists report the length of the working electrode connection alongside the measured resistance (multimeter or EIS) to facilitate this standardization across the field.

show abstract

Section: Introductionmentioning

confidence: 99%

Electrochemical Improvements Can Be Realized via Shortening the Length of Screen-Printed Electrochemical Platforms

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…173 In GQD-based aptasensors, π–π stacking within the layers of GQDs facilitates interactions between their carboxyl groups with the amine groups from aptamers. 174 One such aptasensor for hepatitis C virus (HCV) displayed high reproducibility (standard deviation <2.3%) and recovery in serum (99–109%). 106 Such π–π interactions between sp 2 hybridized carbon nanoparticles and nucleotides also result in fluorescence resonance energy transfer (FRET).…”

Section: Carbon Allotrope-based Nanomaterials In Virus Sensing and Tr...mentioning

confidence: 99%

Nanomaterials for virus sensing and tracking

Pirzada

Altıntaş

2022

Chem. Soc. Rev.

View full text Add to dashboard Cite

show abstract

“…The developed device shows a wider dynamic range of 1.0–500 nM with an LOD of 1 pM [ 105 ]. In 2021, another study was conducted for the detection of HIV, targeting a different binding site, i.e., p24-HIV protein-using aptasensor technology [ 106 ]. The aptasensor was designed by using a screen-printed electrode which was modified through the dispersion of graphene quantum dots (GQD) by electrodeposition.…”

Section: Label and Label-free Aptasensorsmentioning

confidence: 99%

Ultrasensitive Aptasensors for the Detection of Viruses Based on Opto-Electrochemical Readout Systems

et al. 2022

View full text Add to dashboard Cite

Viral infections are becoming the foremost driver of morbidity, mortality and economic loss all around the world. Treatment for diseases associated to some deadly viruses are challenging tasks, due to lack of infrastructure, finance and availability of rapid, accurate and easy-to-use detection methods or devices. The emergence of biosensors has proven to be a success in the field of diagnosis to overcome the challenges associated with traditional methods. Furthermore, the incorporation of aptamers as bio-recognition elements in the design of biosensors has paved a way towards rapid, cost-effective, and specific detection devices which are insensitive to changes in the environment. In the last decade, aptamers have emerged to be suitable and efficient biorecognition elements for the detection of different kinds of analytes, such as metal ions, small and macro molecules, and even cells. The signal generation in the detection process depends on different parameters; one such parameter is whether the labelled molecule is incorporated or not for monitoring the sensing process. Based on the labelling, biosensors are classified as label or label-free; both have their significant advantages and disadvantages. Here, we have primarily reviewed the advantages for using aptamers in the transduction system of sensing devices. Furthermore, the labelled and label-free opto-electrochemical aptasensors for the detection of various kinds of viruses have been discussed. Moreover, numerous globally developed aptasensors for the sensing of different types of viruses have been illustrated and explained in tabulated form.

show abstract

Label-free aptasensor for p24-HIV protein detection based on graphene quantum dots as an electrochemical signal amplifier

Cited by 37 publications

References 33 publications

Electrochemical Improvements Can Be Realized via Shortening the Length of Screen-Printed Electrochemical Platforms

Electrochemical Improvements Can Be Realized via Shortening the Length of Screen-Printed Electrochemical Platforms

Nanomaterials for virus sensing and tracking

Ultrasensitive Aptasensors for the Detection of Viruses Based on Opto-Electrochemical Readout Systems

Contact Info

Product

Resources

About