Purpose: To establish and compare protocols of alkaline cauterization for inducing corneal angiogenesis in murine models. Methods: Twenty-four adult Wistar rats were distributed into four groups (G1, G2, G3, and G4). The right eye cornea from each rat was cauterized using filter paper (3 mm), soaked in a solution of silver and potassium nitrates (3:1). Cauterization times were 10 (G1 and G4), or 20 seconds (G2 and G3). Cauterized corneas were washed with Ringer's lactate solution. The filter paper was either removed before washing (G1 and G2), or kept on the corneas (G3 and G4). Corneas were photographed at multiple time points (2, 4, 6, 8, 11, 13, and 15 days after the procedure), and neovascularization parameters were assayed. Results: Neovascularization was observed in 66% of G1 corneas, and 100% of G2, G3, and G4 corneas. On day 15, G1 corneas showed smaller vascularized areas (12.63 ± 12.59%) compared to those in the G3 (41.95 ± 17.32%) and G4 (33 ± 11.74%) (P < 0.05) groups. Conclusions: The silver and potassium nitrate solution effectively induced corneal angiogenesis. The G2, G3, and G4 protocols showed excellent reproducibility, and induced vascularization in 100% of corneas.
The aim of this research was to evaluate the presence of persistent pupillary membrane (PPM) in rats. Thirty rats between three and four months of age and weighing 300-500 grams, provided by the biothery section of the General Administration at Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil, were subjected to ophthalmological examination by slit lamp biomicroscopy, fluorescein eye stain test and rebound tonometry. We found PPM with possible hereditary origin in 15 animals (50%).
To evaluate renal repair in rats who had renal infarction induced by the obstruction of blood flow in the renal artery and were treated with transplantation of adipose tissue derived mesenchymal stem cell Methods: 16-week-old Wistar rats (n=72) were used, submitted to celiotomy and had of the renal artery and vein clipped for 24 hours. The animals were randomly assigned to 10 experimental homogeneous groups, corresponding to the treatments with phosphatebuffered saline (PBS) or adipose tissue derived mesenchymal stem cell (ADSC), duration of application (24 or 48 hours), and site of transplantation (lateral vein of the tail or intrarenal). After the treatments were performed, at 8 and 31 days, four animals in each group were subjected to left nephrectomy for histological studies. Results: Histologically, a higher amount of cell debris and tubules devoid of the epithelium and a higher degree of necrosis were observed in the groups treated with PBS, as opposed to a low degree of necrosis and higher tubular vascularization in the groups treated with ADSC, particularly in the group treated with intrarenal ADSC 48 hours after injury. Conclusion: The transplantation of ADSC positively contributed to the replacement of necrotic tissue by renal tubular cells, vascularization of the renal parenchyma, and restoration of the organ function.
Abstract:In this research we evaluated the supramolecular organizations and the optical anisotropical properties of the de-epithelialized human amniotic membrane and rabbit limbal stroma, before and after explant culture. Birefringence, monochromatic light spectral absorption and linear dichroism of the main extracellular matrix biopolymers, that is, the fibrillar collagens and proteoglycans, were investigated by polarized light microscopy combined with image analysis. Our results demonstrated that the culture procedure-induced stimuli altered the supra-organizational characteristics (in terms of collagens/proteoglycans spatial orientation and ordered-aggregational state) of the amniotic and limbal extracellular matrix, which led to changes in optical anisotropical properties. and extracellular matrix alignment on human mesenchymal stem cells invasion into decellularized engineered tissues," J. Tissue Eng. Regen. Med. 9(5), 605-618 (2015). 10. E. Mattia and S. Otto, "Supramolecular systems chemistry," Nat. Nanotechnol. 10(2), 111-119 (2015). 11. P. Rifes and S. Thorsteinsdóttir, "Extracellular matrix assembly and 3D organization during paraxial mesoderm development in the chick embryo," Dev. Biol. 368(2), 370-381 (2012). 12. K. Burridge and M. Chrzanowska-Wodnicka, "Focal adhesions, contractility, and signaling," Annu. Rev. Cell Dev. Biol. 12(1), 463-519 (1996).
The objective of this study was to perform examinations of canine fecal samples collected in the urban region, focusing on enteroparasitological diseases, common zoonosis that have their risk of transmission increased due to the growing number of stray dogs in these areas. One hundred and twenty fecal samples of stray dogs were collected from various locations of the studied region and processed by the method of Mollay Willis and Hoffman. Fifty local residents were interviewed, using a semi-structured questionnaire containing questions concerning personal hygiene, stray dogs as a risk factor and parasitological diseases knowledge. Parasitological contamination was observed in 75.9% of the samples; the main parasite was Ancylostoma spp. (72.5%), followed by Toxocara spp. (8.3%). In 24.1% of the samples, less relevant or non-important zoonosis helminths were found. Of those interviewed, only three (6%) were aware of the risk of parasitic contamination by dog feces. The high rate of dogs with infection by enteroparasites along with a lack of knowledge of the population about these infections represents a high risk of zoonosis and zooanthroponosis.
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