Effect of metronidazole ophthalmic solution on corneal neovascularization in a rat model

Claros-Chacaltana, Flor Diana Yokoay; Aldrovani, Marcela; Kobashigawa, Karina Kamachi; Padua, Iván Ricardo Martínez; Valdetaro, Gisele Pereira; Sobrinho, Alexandre Augusto Franchi de Barros; Abreu, Thaís Guimarães Morato; Laus, José Luiz

doi:10.1007/s10792-018-0922-2

Cited by 4 publications

(2 citation statements)

References 45 publications

(70 reference statements)

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“…The maximum reepithelialization and faster corneal haling was previously reported for corneal ulcer after treating rats cornea with 0.1% metronidazole ophthalmic solution for seven days after injury. The authors concluded that metronidazole accelerate the corneal healing via modulating tissue angiogenesis as it has proangiogenic action [ 107 , 108 ]. It was previously reported by Trindade and his colleagues that topical application of 4% metronidazole solution had a direct action on fibroblasts via activating the development of keratinocytes and stimulating the reepithelialization and wound healing on the third day [ 69 ].…”

Section: Resultsmentioning

confidence: 99%

Metronidazole Topically Immobilized Electrospun Nanofibrous Scaffold: Novel Secondary Intention Wound Healing Accelerator

et al. 2022

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The process of secondary intention wound healing includes long repair and healing time. Electrospun nanofibrous scaffolds have shown potential for wound dressing. Biopolymers have gained much attention due to their remarkable characteristics such as biodegradability, biocompatibility, non-immunogenicity and nontoxicity. This study anticipated to develop a new composite metronidazole (MTZ) immobilized nanofibrous scaffold based on poly (3-hydroxy butyrate) (PHB) and Gelatin (Gel) to be utilized as a novel secondary intention wound healing accelerator. Herein, PHB and Gel were mixed together at different weight ratios to prepare polymer solutions with final concentration of (7%), loaded with two different concentrations 5% (Z1) and 10% (Z2) of MTZ. Nanofibrous scaffolds were obtained by manipulating electrospinning technique. The properties of MTZ immobilized PHB/Gel nanofibrous scaffold were evaluated (SEM, FTIR, TGA, water uptake, contact angle, porosity, mechanical properties and antibacterial activity). Additionally, in vitro cytocompatibility of the obtained nanofibrous scaffolds were assessed by using the cell counting kit-8 (CCK-8 assay). Moreover, in vivo wound healing experiments revealed that the prepared nanofibrous scaffold highly augmented the transforming growth factor (TGF-β) signaling pathway, moderately suppressed the pro-inflammatory cytokine (IL-6). These results indicate that MTZ immobilized PHB/Gel nanofibrous scaffold significantly boost accelerating secondary intention wound healing.

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Section: Resultsmentioning

confidence: 99%

Metronidazole Topically Immobilized Electrospun Nanofibrous Scaffold: Novel Secondary Intention Wound Healing Accelerator

et al. 2022

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“…The use of a 0.75% w / w metronidazole (MTZ) topical gel resulted in an improvement in rosacea-related eyelid health [ 15 ]. The pharmacokinetic properties of MTZ administered in a drop formulation exhibited slow penetration through the lipophilic and hydrophilic epithelial membranes of the cornea, suggesting that this product can only be used for the treatment of infections on the anterior surface(s) of the eye [ 16 ], as the topical administration of MTZ reduces neovascularization of the cornea and improves the treatment of ocular rosacea [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Chitosan-Coated SLN: A Potential System for Ocular Delivery of Metronidazole

et al. 2023

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Ophthalmic drops for ocular delivery exhibit inadequate residence time, which often requires multiple daily dosing that may result in patient non-adherence. In this study, the development of a once-daily-dosed chitosan-coated metronidazole (MTZ)-loaded solid lipid nanoparticles (SLNs) for ocular delivery was undertaken. Melt emulsification and ultrasonication were used to manufacture MTZ-loaded SLN, which were subsequently coated with chitosan (CS) by mechanical stirring using a 0.1% w/v solution. Gelucire® 48/16 and Transcutol® HP were used as the solid lipid and synthetic solvent, respectively, with Tween® 20 included as a stabilizing agent. The critical quality attributes (CQA) of the optimized CS-coated SLN that was monitored included particle size, polydispersity index, Zeta potential, % entrapment efficiency, % MTZ loading, pH, and osmolarity. The optimized coated nanocarriers were evaluated using laser Doppler anemometry (LDA) and were determined to be stable, with particle sizes in the nanometre range. In vitro mucoadhesion, MTZ release and short-term stability, in addition to the determination of the shape of the optimized CS-coated SLN, were undertaken. The mucoadhesive properties of the optimized CS-coated MTZ-loaded SLN demonstrated increased ocular availability, which may allow dose reduction or longer intervals between doses by improving precorneal retention and ocular availability. Overall, our findings suggest that CS-coated MTZ-loaded SLNs have the potential for clinical application, to enhance ocular delivery through the release of MTZ.

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Effect of treatment with silver(I) complex of metronidazole on ocular rosacea: Design and formulation of new silver drug with potent antimicrobial activity

Waszczykowska

Żyro

Jurowski

et al. 2020

Journal of Trace Elements in Medicine and Biology

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Effect of metronidazole ophthalmic solution on corneal neovascularization in a rat model

Abstract: Regular instillation of 0.1 or 0.5% metronidazole had a significant inhibitory effect for CNV on chemical burns induced in a rat model.

Cited by 4 publications

References 45 publications

Metronidazole Topically Immobilized Electrospun Nanofibrous Scaffold: Novel Secondary Intention Wound Healing Accelerator

Metronidazole Topically Immobilized Electrospun Nanofibrous Scaffold: Novel Secondary Intention Wound Healing Accelerator

Chitosan-Coated SLN: A Potential System for Ocular Delivery of Metronidazole

Effect of treatment with silver(I) complex of metronidazole on ocular rosacea: Design and formulation of new silver drug with potent antimicrobial activity

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