Metronidazole Topically Immobilized Electrospun Nanofibrous Scaffold: Novel Secondary Intention Wound Healing Accelerator

Abstract

The process of secondary intention wound healing includes long repair and healing time. Electrospun nanofibrous scaffolds have shown potential for wound dressing. Biopolymers have gained much attention due to their remarkable characteristics such as biodegradability, biocompatibility, non-immunogenicity and nontoxicity. This study anticipated to develop a new composite metronidazole (MTZ) immobilized nanofibrous scaffold based on poly (3-hydroxy butyrate) (PHB) and Gelatin (Gel) to be utilized as a novel secondary intention wound healing accelerator. Herein, PHB and Gel were mixed together at different weight ratios to prepare polymer solutions with final concentration of (7%), loaded with two different concentrations 5% (Z1) and 10% (Z2) of MTZ. Nanofibrous scaffolds were obtained by manipulating electrospinning technique. The properties of MTZ immobilized PHB/Gel nanofibrous scaffold were evaluated (SEM, FTIR, TGA, water uptake, contact angle, porosity, mechanical properties and antibacterial activity). Additionally, in vitro cytocompatibility of the obtained nanofibrous scaffolds were assessed by using the cell counting kit-8 (CCK-8 assay). Moreover, in vivo wound healing experiments revealed that the prepared nanofibrous scaffold highly augmented the transforming growth factor (TGF-β) signaling pathway, moderately suppressed the pro-inflammatory cytokine (IL-6). These results indicate that MTZ immobilized PHB/Gel nanofibrous scaffold significantly boost accelerating secondary intention wound healing.

Integration of Bioglass Into PHBV-Constructed Tissue-Engineered Cartilages to Improve Chondrogenic Properties of Cartilage Progenitor Cells

“…ImageJ software was used to analyze/process the SEM images of the scaffolds to obtain the porosity and pore size distribution data. The mass of the scaffolds and dimensions were measured to analyze the porosity ratio of the 3D porous bioactive scaffolds as previously described ( El-Shanshory et al, 2022 ). The compressive strength of the 3D bioactive scaffolds was determined according to the force–displacement curve with a Shimadzu AG mechanical tester ( Wright et al, 2022 ) (Shimadzu Co., Japan).…”

Electrospun nanofiber-based glucose sensors for glucose detection

“…It has drawn increasing attention recently because active ingredients (including those for detecting glucose) can be easily encapsulated into the polymeric nanofibers to form a new kind of functional nanocomposites ( Wang M. et al, 2021 ; Zhang M. et al, 2022 ; Zhou Y. et al, 2022 ). Based on this strategy, electrospun nanofibers have found potential applications in almost all types of scientific fields, such as energy, environment, medicine, tissue engineering, food, and agriculture ( Wang Y. et al, 2022 ; Zhang et al, 2022d ; El-Shanshory et al, 2022 ; Guo et al, 2022 ; Song et al, 2022 ; Yuan et al, 2022 ). Its popularity has a close relationship with its capability to create nanofibers, and also because this is a nano era ( Yu, 2021 ; Yu and Lv, 2022 ).…”

Hybrid Films Prepared from a Combination of Electrospinning and Casting for Offering a Dual-Phase Drug Release

“…Successful drug delivery needs supports from both pharmaceutical excipients, pharmaceutical techniques, and their effective combinations [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ]. To a certain extent, the fields of pharmaceutics and drug delivery rely heavily on the inputs of materials sciences (new excipients) and engineering (new techniques) [ 8 , 9 , 10 , 11 , 12 , 13 ]. Thus, during the past half a century, on the one hand, more and more types of materials (including natural polymers, synthesis polymers, phospholipid, surfactants, and even inorganic materials) have been tried as new drug carriers for adjusting the drug release performances [ 14 , 15 , 16 , 17 , 18 ].…”