Persistent papillary membrane in Wistar laboratory rats ( Rattus Norvegicus, Albinus Variation, Wistar )

Chacaltana, Flor Diana Yokoay Claros; Kobashigawa, Karina Kamachi; Padua, Iván Ricardo Martínez; Valdetaro, Gisele Pereira; Aldrovani, Marcela; Laus, José Luiz

doi:10.1590/0103-8478cr20160421

Cited by 6 publications

(8 citation statements)

References 25 publications

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“…Ophthalmological examination (Week 13) identified two incidences of corneal opacity in the control (one per sex) and three in high-dose males, in addition four incidences of persistent pupillary membrane in the control (one male, three females) and the high-dose treatment group (three males, one female) were observed. Both optical abnormalities have been reported to occur spontaneously and in high frequencies in Wistar and Han rats [35,36]. Ocular examination results were not statistically significant for either sex during the pre-test and at the end of the treatment period; thus the isolated incidences were not attributed to L-5-MTHF-Ca administration.…”

Section: Resultsmentioning

confidence: 88%

Safety evaluation of calcium L-methylfolate

Niederberger¹,

Dahms

Broschard

et al. 2019

Toxicology Reports

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Section: Resultsmentioning

confidence: 88%

Safety evaluation of calcium L-methylfolate

Niederberger¹,

Dahms

Broschard

et al. 2019

Toxicology Reports

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“…Hyaloid vessels and pupillary membrane (PM) form a temporary capillary network in the anterior chamber, iris diaphragm, and lens. This network nourishes the immature lens, retina, and vitreous humour during morphogenesis [30,31,32]. In the 11-day old rabbit, the PM is still functional.…”

Section: Discussionmentioning

confidence: 99%

“…In the 11-day old rabbit, the PM is still functional. Functional vessels had disappeared in the centre of the pupil in the 20-day-old rabbits [32]. As E. cuniculi is disseminated via the blood, the lens can be directly infected by E. cuniculi not only in utero [11,12], but also shortly postnatally.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Detection of Encephalitozoon cuniculi in Ocular Structures of Immunocompetent Rabbits

Jeklová

Levá

Kummer

et al. 2019

Animals

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Simple SummaryEncephalitozoonosis is a common infectious disease widely spread among rabbits. Its causative agent, Encephalitozoon cuniculi, is considered to be transmissible to humans. In rabbits, clinical signs include discoordination, head tilt, excessive water intake, excessive urination and cataracts. This study investigates, for the first time, whether the E. cuniculi organism can be detected in ocular structures in healthy adult rabbits after experimental oral infection using immunohistochemistry—detection of the organism in the tissue using a specific staining method. In infected animals, E. cuniculi spores were detected in many ophthalmic structures (periocular connective tissue, sclera, cornea, choroidea, iris, retina and lens) as early as 2 weeks after infection. There were no signs of inflammatory lesions in any of the ocular tissues examined at 2, 4, 6 and 8 weeks after infection. E. cuniculi was also detected in the lens of adult rabbits, which indicates that ways of lens infection other than intrauterine and haematogenic are possible. This information can help to understand E. cuniculi dissemination to various ocular tissues structures after oral infection.AbstractEncephalitozoonosis is a common infectious disease widely spread among rabbits. Its causative agent, Encephalitozoon cuniculi, is considered as a zoonotic and emerging pathogen capable of infecting both immunocompetent and immunocompromised hosts, including humans. In rabbits, clinical signs include neurological, kidney and ocular disease. The aim of this study was to detect E. cuniculi in ocular structures in immunocompetent rabbits after experimental oral infection using immunohistochemistry. In infected animals, E. cuniculi spores were present in periocular connective tissue, sclera, cornea, choroidea, iris, retina and lens, as a round to ovoid organism reacting with a specific anti-E. cuniculi monoclonal antibody as early as 2 weeks after infection. There were no signs of inflammatory lesions in any of the ocular tissues examined at 2, 4, 6 and 8 weeks after infection. In the present study, E. cuniculi was also detected in the lenses of adult rabbits, which indicates that ways of lens infection other than intrauterine and haematogenic are possible.

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“…Forty male Wistar rats (Rattus norvegicus), aged between 3 and 4 months, weighing 428.40 ± 63.60 g (mean ± SD), were selected for the study. All the subjects were free of signs of corneal disorders [23].…”

Section: Animalsmentioning

confidence: 99%