Effects of intracameral ascorbic acid on the corneal endothelium of dogs undergoing phacoemulsification

Padua, Iván Ricardo Martínez; Valdetaro, Gisele Pereira; Lima, Tiago Barbalho; Kobashigawa, Karina Kamachi; Silva, Paloma E.S.; Aldrovani, Marcela; Padua, Pedro P. M.; Laus, José Luiz

doi:10.1111/vop.12490

Cited by 9 publications

(11 citation statements)

References 33 publications

(44 reference statements)

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“…In the present study, the time in patients with mature cataracts (G2) was higher (4.94min), but lower than that reported by the same author. It is a consensus that shorter time is desirable, since the use of too much ultrasound function of the phacoemulsificator can lead to endothelial damage and increased intraocular inflammation (Wilkie & Colitz, 2007;Munger, 2009;Padua et al, 2017). It should be noted that the statistical analysis revealed significant differences between the groups.…”

Section: Discussionmentioning

confidence: 96%

“…Furthermore, cataracts in dogs present cataractous nuclei with variable degrees of hardness (Slatter, 2005;Munger, 2009), which are not always removable using the "Divide and conquer" technique, faced with trans-surgical complications, such as difficulty with nucleus rotation to perform the second fracture (Akahoshi, 1998;Slatter, 2005;Warren, 2004). Furthermore, due to the technical impossibility, irregular fragments can be created which are free in the anterior chamber, increasing the difficulty in performing emulsification and aspiration and thus the degree of endothelial injury and intraocular inflammation (Munger, 2009;Padua et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…The only therapeutic approach, in humans and in animals, is surgical (Fischer, 1989;Dziezyc, 1990;La Croix, 2008;Resnikoff et al, 2004), although Zhao et al (2015) have described that lanosterol decreases the aggregates of lenticular proteins and reduces the chances of cataract evolution, pointing it as a new treatment strategy. In the veterinary cataract surgery has evolved from extracapsular extraction to phacoemulsification (La Croix, 2008;Padua et al, 2017). The advances are unquestionable, and more alternatives are being sought to further improve the methods.…”

Section: Introductionmentioning

confidence: 99%

“…This technique is called "V-prechop". The evaluation of postoperative clinical parameters may help to predict whether or not the technique is proposed, as well as specular microscopy (Munger, 2009;Pigatto et al, 2006;Padua et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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V-Prechop nucleodissection technique feasibility in the phacoemulsification in dogs with cataracts. Clinical aspects and specular microscopy

Andrade¹,

Araçatuba²,

Conceição³

et al. 2020

Semina: Ciênc. Agrár.

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The aim of this study was to evaluate the feasibility of the V-Prechop nucleodissection technique in the phacoemulsification in dogs and the clinical aspects and of the specular microscopy in the postoperative period. Fourty three dogs of different breeds, males and females, aged 3 to 10 years, with mature (n=22) and immature (n=21) cataracts were used. After surgery, patients were evaluated weekly for visual acuity, intraocular pressure, and endothelial corneal cell density (by non-contact specular microscopy) and quantitative flare (by laser flare photometry) during different periods. The "V-prechop" technique presented technical difficulties in implementation in patients with a mature cataract. In selected cases of patients with an immature cataract, the technique can be employed, as the nuclei are softer, allowing confection of the linear fragments in “V” to be performed. In addition, the eyes of dogs with a mature cataract presented more intensive postoperative uveitis, probably related to the greater difficulty in conducting the "V" nucleodissection. There was decreased endothelial corneal cell density in dogs with mature and immature cataracts. This occurrence was greater in patients with a mature cataract, given the increased intraocular manipulation and surgical time due to the difficulty in performing the “V” nucleodissection. According to the results obtained, the “V-prechop” nucleodissection technique can be indicated in selective cases of dogs with an immature cataract.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

V-Prechop nucleodissection technique feasibility in the phacoemulsification in dogs with cataracts. Clinical aspects and specular microscopy

Andrade¹,

Araçatuba²,

Conceição³

et al. 2020

Semina: Ciênc. Agrár.

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“…Corneal endothelial cell density (ECD) tends to be decreased by phacoemulsification-induced oxidative stress. It has been demonstrated in a canine model that intracameral infusion of ascorbic acid during phacoemulsification minimizes the loss of corneal endothelial cells [34]. Nevertheless, the protective effect of topically administered ascorbic acid on the corneal endothelium has not yet been defined.…”

Section: Discussionmentioning

confidence: 99%

Topical Ascorbic Acid Ameliorates Oxidative Stress-Induced Corneal Endothelial Damage via Suppression of Apoptosis and Autophagic Flux Blockage

Hsueh

Meir

Yeh

et al. 2020

Cells

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Compromised pumping function of the corneal endothelium, due to loss of endothelial cells, results in corneal edema and subsequent visual problems. Clinically and experimentally, oxidative stress may cause corneal endothelial decompensation after phacoemulsification. Additionally, in vitro and animal studies have demonstrated the protective effects of intraoperative infusion of ascorbic acid (AA). Here, we established a paraquat-induced cell damage model, in which paraquat induced reactive oxygen species (ROS) production and apoptosis in the B4G12 and ARPE-19 cell lines. We demonstrate that oxidative stress triggered autophagic flux blockage in corneal endothelial cells and that addition of AA ameliorated such oxidative damage. We also demonstrate the downregulation of Akt phosphorylation in response to oxidative stress. Pretreatment with ascorbic acid reduced the downregulation of Akt phosphorylation, while inhibition of the PI3K/Akt pathway attenuated the protective effects of AA. Further, we establish an in vivo rabbit model of corneal endothelial damage, in which an intracameral infusion of paraquat caused corneal opacity. Administration of AA via topical application increased its concentration in the corneal stroma and reduced oxidative stress in the corneal endothelium, thereby promoting corneal clarity. Our findings indicate a perioperative strategy of topical AA administration to prevent oxidative stress-induced damage, particularly for those with vulnerable corneal endothelia.

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New Ultra-Structural Aspects of Cornea: Significance of Ascorbate Micronutrient Deficiency

Serra

Suárez

2019

Handbook of Nutrition, Diet, and the Eye

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Effects of intracameral ascorbic acid on the corneal endothelium of dogs undergoing phacoemulsification

Abstract: According to the results obtained, ascorbic acid minimizes cellular losses in the corneal endothelium.

Cited by 9 publications

References 33 publications

V-Prechop nucleodissection technique feasibility in the phacoemulsification in dogs with cataracts. Clinical aspects and specular microscopy

V-Prechop nucleodissection technique feasibility in the phacoemulsification in dogs with cataracts. Clinical aspects and specular microscopy

Topical Ascorbic Acid Ameliorates Oxidative Stress-Induced Corneal Endothelial Damage via Suppression of Apoptosis and Autophagic Flux Blockage

New Ultra-Structural Aspects of Cornea: Significance of Ascorbate Micronutrient Deficiency

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