The new coumarin, (+j-(2'S,3'/?)-3-hydroxymarmesin [1], was isolated from the aqueous fraction of the EtOH extract of the root bark of Brosimum gaudichaudii (Moraceae).
Supercritical fluid extraction with COz, performed in a home-made system, of rhizomes of Dorstenia bryoniifolia Mart. ex Miq. (Moraceae) and of bark roots of Brosimum gaudichaudii Trecul (Moraceae) afforded crude extracts that were analysed by high resolution gas chromatography (HRGC). The D. bryoniifolia extract contained, besides the previously reported pimpinelin and isohergapten, the furocoumarins psoralen, bergapten, isopimpinelin and the triterpenes a-and 9-amyrin and the acetate of the latter. The B . gaudichaudii extract contained a number of terpenoids as well as the previously reported psoralen and bergapten. Supercritical fluid extraction gave extracts qualitatively similar to those obtained by Soxhlet extraction with hexane and, together with off-line HRGC, was shown to be a fast and accurate technique to be used in rapid phytochemical examination.
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