One critical element for applying atmospheric pressure plasma jet for medical purposes is that it is possible to construct a combinatorial therapeutic regimen based on contact and noncontact styles for the cold atmospheric plasma jet. This study evaluates plasma jet effectiveness for bacteria-infected wounds in a small animal model. In this investigation, we test a novel combinative treatment using contact and noncontact style for plasma jet that was generated at high voltage of ~ 9 kV. We use medical-grade argon gas as a single carrier gas. The object of plasma treatment is BALB/c mouse skin wounds that were infected with Staphylococcus aureus. We use four plasma jet treatments, namely, C (control), CP-CP (contact), NCP-NCP (noncontact), and CP-NCP (contact-noncontact). For CP-NCP, from days 0 to 7 we apply a contact style of plasma jet treatment to wounds to kill bacteria; from days 8 to 13, a noncontact style of plasma jet is applied to stimulate wound healing. Our results show that with CP-CP, contact plasma treatment can remove the biofilm layer, but after the biofilm layer disappears contact plasma treatment inhibits the wound-healing process. NCP-NCP is not effective in eliminating bacterial biofilms and impedes the wound-healing process. With CP-NCP, contact plasma exposure during days 0 to 7 is able to remove bacterial biofilms, and irradiation of noncontact plasma during days 8 to 14 accelerates wound healing. Finally, CP-NCP significantly accelerates healing. The combinatorial therapeutic regimen based on contact and noncontact styles of cold atmospheric plasma jet is recommended for chronic wound management, because it effectively removes bacterial biofilms and accelerates wound healing.

Symptoms of a disease can be prevented if routine health checks are done. Health checks can be carried out in public and private laboratories, or free checks conducted by health education institutions through community service activities. The implementation of community service is carried out by lecturers involving students of the Medical Laboratory Science at the Muhammadiyah University of Semarang. This community service activity was carried out on December 15, 2019 in front of the Semarang Health Training Center Office on December 15, 2019. The inspection method uses Point of Care testing (POCT). The results of the examination that the number of respondents with cholesterol levels above normal for women more than men, while glucose and uric acid levels above normal in men more than women. The average cholesterol, glucose, and uric acid levels for men were 224.2; 123.1; 4.8 mg/dL respectively, while for women is 194.5; 121.2; 6,0 mg/dL. The higher age (12 - 65 years), the average cholesterol level increases from 189 to 217.8 mg/dL (15.25%) and the average glucose level increase from 84.4 to 131.5 mg / dL (55.80%), while the average uric acid level decreases from 5.4 to 5.2 mg / dL (3.85%). The conclusions of the health inspection activity about the result of cholesterol, glucose, and uric acid levels can be used to check public health

The medical aspect of plasma conceptually is concerned with the ability of plasma to produce biological molecules, such as RONS, which can be physically controlled through dose and behavior. RONS in micro concentrations was reported to have wound healing properties. The effect of a plasma jet on chronic skin wounds of mice on day 14 which theoretically included the remodeling phase has not been widely studied. The aim of the study was to calculate the number of inflammatory cells, fibroblasts, blood vessels, the percentage of re-epithelization, and the percentage of necrosis. Experimental research method with an analytic descriptive design. The results of microscopic observations showed that based on the number of inflammatory cells (round cells) data, the control group was significantly higher than the non-contact plasma (P<0.05). Based on the blood vessel count data, it was found that the control group was significantly higher (P<0.05) than the contact group, non-contact group and non-contact group. Based on the data on the number of fibroblasts, the results in contact-non-contact group were significantly (P<0, 05) higher than control group and plasma-contact group. Based on the data on the percentage of reepithelialization, the results were different in all treatment groups but not significant (P>0.05). Based on the data on  the percentage of necrosis, the results were were different in all treatment groups but not significant (P>0.05).

Hepatitis B merupakan penyakit inflamasi dan nekrosis sel hati yang disebabkan oleh infeksi virus hepatitis B (VHB). Kehadiran anti-HBs merupakan faktor penting dalam diagnosis dan prognosis infeksi VHB. Rapid test adalah salah satu metode pemeriksaan selain ELISA untuk mendeteksi adanya anti-HBs, lebih praktis dalam penggunaan dan ekonomis. Tujuan penelitian ini untuk mengetahui nilai sensitivitas dan spesifisitas rapid test anti-HBs Intec® terhadap metode ELISA sebagai gold standar. Penelitian ini merupakan studi observasional menggunakan metode uji diagnostik. Populasi penelitian ini adalah mahasiswa D4 Analis Kesehatan FIKKES Universitas Muhammadiyah Semarang semester tujuh post vaksinasi hepatitis B sejumlah 32 orang untuk didapatkan serum darah. Analisis statistik dilakukan uji Mc Nemar. Hasil pemeriksaan dengan rapid test yaitu positif 26 sampel (81,2%), dan  negatif 6 sampel (18,8%). Hasil pemeriksaan dengan ELISA yaitu reaktif 27 sampel (84,4%), dan nonreaktif 5 sampel (15,6%). Kesimpulan akhir yaitu Rapid test anti-HBs Intec® memiliki nilai sensitivitas dan spesifisitas yang sangat baik dengan persentase sensitivitas 96,3% dan persentase spesifisitas 100%. Tidak terdapat perbedaan yang bermakna pada sensitivitas dan spesifisitas metode rapid test dan ELISA (p= 1,000) sehingga Rapid test anti-HBs Intec®  dapat digunakan sebagai skrining untuk pemeriksaan anti-HBs.

Latar belakang: Anemia masih menjadi masalah global, termasuk pada remaja di Indonesia. Salah satu dampak anemia yaitu penurunan imunitas yang mana dapat berpengaruh pada status kesehatan remaja selama masa transisi menuju endemi COVID-19. Tujuan: Melakukan penyuluhan kesehatan tentang anemia dan pemeriksaan hemoglobindeteksi anemia pada remaja di Panti Asuhan Darul Falah Al-Hasyimiyah. Metode: Kegiatan dilakukan pada bulan Februari 2023 berupa edukasi tentang anemia dan pemeriksaan hemoglobin pada anak-anak dan remaja di Panti Asuhan Darul Falah Al-Hasyimiyah. Tahap pelaksanaan dimulai dengan observasi lapangan, rencana penyusunan program, pelaksanaan dimulai dengan edukasi dan pemeriksaan Hb, dan evaluasi berupa pre-test dan post-test. Hasil: Tahap observasi didapatkan data awal total anak-anak dan remaja berjumlah 36 orang yang sebagian besar masih asing tentang anemia. Tahap edukasi anemia didapatkan peningkatan pengetahuan responden yang signifikan. Selanjutnya pada pemeriksaan hemoglobin ditemukan 9 remaja (25%) yang mengalami gejala anemia dan 27 remaja (75%) yang memiliki kadar Hb normal. Kesimpulan: Edukasi dapat meningkatkan pengetahuan tentang anemia secara signifikan. Ada 9 orang remaja (25%) mengalami anemia dan 27 orang remaja (75%) dengan kadar Hb normal. Kata kunci: anemia, endemi covid-19, hemoglobin, penyuluhan __________________________________________________________________________________________ Abstract Background: Anemia is still a global major issue, particularly among adolescents in Indonesia. Anemia causes a decrease in immunity, which can affect the health of adolescents during the transition to the COVID-19 endemic. Objective: Conduct health education about anemia and hemoglobin examination to detect anemia in adolescents at the Darul Falah Al-Hasyimiyah Orphanage. Method: The activity was carried out in February 2023 including anemia education and hemoglobin checks for children and adolescents at the Darul Falah Al-Hasyimiyah Orphanage. The activity phase begins with field observations, programming plans, implementation begins with education and Hb-testing, and evaluation namely pre-test and post-test. Result: The observation phase collected preliminary data on the total number of children and adolescents 36, the majority of whom were unfamiliar with anemia. The respondent's knowledge increased significantly during the anemia education stage. Furthermore, the hemoglobin test showed 9 adolescents (25%) who had anemia symptoms and 27 adolescents (75%) who had normal Hb levels. Conclusion: Education can significantly increase anemia knowledge. There were 9 (25%) anemic adolescents and 27 (75%) with normal Hb levels. Keywords: anemia, covid-19 endemic, hemoglobin, counseling