Accelerated Healing of Chronic Wounds under a Combinatorial Therapeutic Regimen Based on Cold Atmospheric Plasma Jet Using Contact and Noncontact Styles

Darmawati, Sri; Nasruddin, Nasruddin; Putri, Gela Setya Ayu; Iswara, Arya; Kurniasiwi, Putri; Wahyuningtyas, Eka Sakti; Nurani, Laela Hayu; Hayati, Defi Nurul; Ishijima, Tatsuo; Nakatani, Toshio; Sugama, Junko

doi:10.1615/plasmamed.2021039083

Cited by 6 publications

(5 citation statements)

References 17 publications

(36 reference statements)

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“…Re-epithelialization is the process of epithelial cells covering the wound by resurfacing the wound with new epithelium. This is different from the study by Putri et al (2021) which reported reepithelialization during the remodeling phase. The remodeling or maturation phase is the final stage in the wound healing process.…”

Section: Discussioncontrasting

confidence: 99%

“…The remodeling or maturation phase is the final stage in the wound healing process. Epithelial cells in this phase have completely covered the edges of the wound until they reach above 90% (Putri et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Observation of re-epithelialization with the help of DinoCapture 2.0 software. The percentage of re-epithelialization was calculated using the formula: 100% × (length of new epithelium/length between the edges of the wound) (Darmawati et al, 2021).…”

Section: Methodsmentioning

confidence: 99%

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Histological Evidence of Wound Healing Process in Chronic Wound Tissue With Medical Plasma Treatment During Proliferative Phase

Putri,

Padanunan

2023

Proceedings of the International Conference of Community Health and Medical Sciences

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Chronic wounds are defined as acute wounds that fail to heal within the expected time. The proliferative phase is one of four major phases in healing physiology that occurs on days 8 to 11, characterized by epithelial cell contraction leading to the edges merging and the wound size decreased. New treatments for chronic wounds have been developed, including medical plasma. Plasma is an ionized gas. Plasma generates RONS molecules, which can be effective in healing chronic wounds but histological view has not been reported. The aim of this study was to calculate the number of inflammatory cells, blood vessels, fibroblasts, the percentage of re-epithelialization, and the thickness of necrosis of chronic wound tissue in mice skin treated with plasma in the proliferation phase. The sample used tissue slides of mice skin with chronic wounds on the 11th day which had been treated with plasma daily. A total of 18 samples were divided into 4 groups. Control (K) chronic wound tissue without treatment, Contact Plasma (CP) chronic wound tissue with 5mm distance of plasma treatment, Contact-Noncontact Plasma (CP-NCP) chronic wound tissue with combination plasma treatment, days 0-6 distance of 5 mm and days 7-13 distance of 20 mm, Non-contact Plasma (NCP) chronic wound tissue with 20mm distance of plasma treatment. The results showed that the highest mean number of fibroblast cells, blood vessels, and percentage re-epithelialization were found in CP-NCP. The highest number of inflammatory cells was in K and the highest thickness of necrosis 70.5µm was found in CP. The final conclusion, the highest average number of fibroblast cells, blood vessels, and percentage of re-epithelialization was found in CP-NCP group indicated the CP-NCP group had the fastest healing process compared to the others. Thus, contact-non-contact plasma medical treatment has potential as a new treatment for chronic wounds.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Histological Evidence of Wound Healing Process in Chronic Wound Tissue With Medical Plasma Treatment During Proliferative Phase

Putri,

Padanunan

2023

Proceedings of the International Conference of Community Health and Medical Sciences

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“…Numerous studies have shown that cold plasma (supplied by various plasma devices) may accelerate wound healing [34][35][36] and decrease bacterial inoculate of both natural wound flora and infected wounds [37,38]. In our study, J-Plasma incisions were found to have no difference in external wound appearance using previously published scoring systems [28,39] when compared to steel scalpel incisions at all time points throughout the study.…”

Section: Discussionsupporting

confidence: 47%

Evaluating the Healing Potential of J-Plasma Scalpel-Created Surgical Incisions in Porcine and Rat Models

Elmore,

Minissale,

Israel

et al. 2024

Biomedicines

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Cold atmospheric plasma devices generate reactive oxygen and nitrogen species that can be anti-microbial but also promote cell migration, differentiation, and tissue wound healing. This report investigates the healing of surgical incisions created using cold plasma generated by the J-Plasma scalpel (Precise Open handpiece, Apyx Medical, Inc.) compared to a steel scalpel in in vivo porcine and rat models. The J-Plasma scalpel is currently FDA approved for the delivery of helium plasma to cut, coagulate, and ablate soft tissue during surgical procedures. To our knowledge, this device has not been studied in creating surgical incisions but only during deeper dissection and hemostasis. External macroscopic and histologic grading by blinded reviewers revealed no significant difference in wound healing appearance or physiology in incisions created using the plasma scalpel as compared with a steel blade scalpel. Incisions created with the plasma scalpel also had superior hemostasis and a reduction in tissue and blood carryover. Scanning electron microscopy (SEM) and histology showed collagen fibril fusion occurred as the plasma scalpel incised through the tissue, contributing to a sealing effect. In addition, when bacteria were injected into the dermis before incision, the plasma scalpel disrupted the bacterial membrane as visualized in SEM images. External macroscopic and histologic grading by blinded reviewers revealed no significant difference in wound healing appearance or physiology. Based on these results, we propose additional studies to clinically evaluate the use of cold plasma in applications requiring hemostasis or when an increased likelihood of subdermal pathogen leakage could cause surgical site infection (i.e., sites with increased hair follicles).

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“…Basically, all wounds are acute wounds can become chronic if the wound healing time exceeds the physiological wound healing time [3]. Most people experience acute wounds, both large and small wounds, and the depth and severity of these wounds are still considered acute wounds during the physiological wound-healing process, so it is very important to treat and prevent acute wounds from turning into chronic wounds [4] [5]. The consequences triggered by a wound include organ dysfunction or dysfunctionality of certain organ parts, an involuntary sympathetic response, hemorrhaging, thrombus formation, microbial infection, and necrosis.…”

Section: Introductionmentioning

confidence: 99%

The Effectiveness of Combination of Piper betle L. ethanol Extract and Manuka Honey Spray Gel to Accelerating Acute Wound Healing

Wahyuningtyas,

Wijayatri,

Handayani

2024

E3S Web Conf.

Self Cite

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Acute wounds are injuries that occur suddenly to tissues that are prone to the risk of being infected. Therefore, acute wound care must be considered carefully. Piper betle leaf contains flavonoids that have a function in the wound healing process, Manuka honey also contains vitamins that can support the healing phase. The combination of Piper betle L. and manuka honey is made into a spray gel preparation because it has advantages over other topical preparations. The purpose of this study is to see how well a combination wound healing spray gel of Piper betle L. and Manuka honey works on an acute wound in mice. This study uses experimental research with a randomized, matched post-test-only control group design. The formulation taken was maceration extraction from Piper betle L. Simplicia powder with 70% ethanol then evaporated to acquire a concentrated concentrate. Spray gel preparations of a combination of Piper betle L extract and manuka honey were made with a concentration of 1% Piper betle L and Manuka honey, 3% Piper betle L and Manuka honey, and 5% Piper betle L and Manuka honey. Balb/c mice that had full-thickness acute wounds were divided into 7 treatment groups, namely SM1, SM2, SM3, M, KP, B, and D. Mice were treated for 14 days and the wound area ratio was calculated and analyzed by ANOVA. The test results showed a significant difference between groups SM3-M, SM5-M, M-SM3, M-SM5, KP-M, B-SM3, and BM with a significance value below 0.05 (p≤0.05) which proves that the spray gel combination of Piper betle L extract and Manuka honey, oxoferin, and base both have activity in accelerating acute wound healing.

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Accelerated Healing of Chronic Wounds under a Combinatorial Therapeutic Regimen Based on Cold Atmospheric Plasma Jet Using Contact and Noncontact Styles

Cited by 6 publications

References 17 publications

Histological Evidence of Wound Healing Process in Chronic Wound Tissue With Medical Plasma Treatment During Proliferative Phase

Histological Evidence of Wound Healing Process in Chronic Wound Tissue With Medical Plasma Treatment During Proliferative Phase

Evaluating the Healing Potential of J-Plasma Scalpel-Created Surgical Incisions in Porcine and Rat Models

The Effectiveness of Combination of Piper betle L. ethanol Extract and Manuka Honey Spray Gel to Accelerating Acute Wound Healing

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