The composition of the essential oil of Neperu nuda L. ssp. ulbipora (Boiss.) Gams (Lamiaceae) was analysed by means of capillary GC and GC-MS. The main components, accounting for 75.1% of the oil, were 4aa,7a,7aa-nepetalactone (37.5%) and 4aa,7~,7afhepetalactone (37.6%). The sesquiterpene fraction amounted to 14.2% and was dominated by caryophyllene oxide (4.4%).
EXPERIMENTAL
Plant MaterialThe leaves and flowering tops of Nepeta nuda ssp. albiflora were collected in July 1994 near &el in southern Turkey. A voucher specimen was deposited in the Herbarium of Ankara University, Faculty of Pharmacy (AEF 19147).
Isolation of the Essential OilThe essential oil was obtained by hydrodistillation for 3 h in a Clevenger-type apparatus. The yield of the oil was 1.1%. based on the dry weight of the sample.Gas Chromatography GC analyses were performed using a Shimadzu GC 14A gas chromatograph with an OV-1 fused silica column (25 m x 0.25 mm i.d., film thickness 0.24 pm) and SE-52 fused silica column (50 m x 0.32 mm i.d., f i l m thickness 0.29 pm). Oven temperature was held at 40°C for 4 min, programmed to 5OoC at a rate of 2"C/min, then held at 50°C for 10 min and programmed to 270°C at a rate of 2"Umin. Injector and detector (FID) temperatures were kept at 250°C. Split ratio was 1:30. Gas Chromatography-Mass Spectrometry GC-MS analysis were camed out with an HP 5890-VG Zabspec GC-MS system on an OV-1 fused silica column (30 m x 0.32 mm id.). Oven temperature was as above; transfer line temperature 220°C; ion source temperature 200°C; carrier gas helium; splitting ratio 1:40; ionization energy 70 eV; trap current 200 PA; scan range 50-700 amu; scan time 2 s.
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