A comparison of matrix resolution method, ratio spectra derivative spectrophotometry and HPLC method for the determination of thiamine HCl and pyridoxine HCl in pharmaceutical preparation

Dinç, Erdal; Kökdil, Gamze; Onur, Feyyaz

doi:10.1016/s0731-7085(00)00308-3

Cited by 43 publications

(21 citation statements)

References 10 publications

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“…Method C: Ratio spectra derivative spectrophotometric method [18][19][20][21] This method involves dividing the zero order spectras of pure drugs by the individual zero order spectra of each of the drugs. The zero order divisor spectras thus obtained are derivitised to obtain ratio derivative spectras that are independent of the concentration of obtain standard calibration curves.…”

Section: Estimation From Marketed Preparationsmentioning

confidence: 99%

Simultaneous Spectrophotometric Methods for Estimation of Cefpodoxime Proxetil and Potassium Clavulanate in Tablet Dosage form

Thomas¹,

Dighe²,

Kothapalli³

et al. 2010

Journal of Pharmaceutical Research

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Three UV spectrophotometric methods have been developed for the simultaneous estimation of Cefpodoxime proxetil (Cef) and Potassium clavulanate (Pot.clav.) in combined tablet dosage forms. The first method involves Q-absorbance ratio method, the sampling wavelengths selected are, 315.0nm (isoabsorptive point) and 233.0 nm (λmax of Cef). The second method is the First order derivative method, the sampling wavelengths selected are 269.0 nm and 240.0 nm for estimation of Cef and Pot.clav. respectively. In both the methods, the linearity range for both Cef and Pot.clav. was in the concentration range of 1.5-50 µg mL -1 . The third method based on ratio derivative spectrophotometry, involves measurement of absorbances at the amplitudes in the first order derivative of the ratio spectra at 246.0 nm and 327.0 nm for Cef and Pot.clav. respectively over the concentration range of 2.5-50 µg mL -1 for both the drugs. The results of the analysis were validated statistically and recovery studies carried out as per ICH guidelines. The developed methods are rapid, precise, accurate, rugged and can be employed for the routine estimation of Cefpodoxime proxetil and Potassium clavulanate in both bulk and tablet dosage form.

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Section: Estimation From Marketed Preparationsmentioning

confidence: 99%

Simultaneous Spectrophotometric Methods for Estimation of Cefpodoxime Proxetil and Potassium Clavulanate in Tablet Dosage form

Thomas¹,

Dighe²,

Kothapalli³

et al. 2010

Journal of Pharmaceutical Research

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“…It is extensively used as a component of single vitamin B complex and multivitamin preparations [2][3][4]. Several analytical methods, including UV and visible spectrophotometric and multivariate analysis [5][6][7], fluorimetric [8][9][10][11][12][13][14][15][16][17][18][19][20][21], high performance liquid chromatography (HPLC) [22][23][24][25][26][27][28][29][30][31][32][33][34][35], UHPLC/MS-MS [36], turbidimetric and nephelometric [37], amperometric [38], and voltammetric methods [39][40][41][42][43][44][45], have been used for the assay of thiamine and its salts in pharmaceutical preparations, food materials and biological fluids. Some of these methods have previously been reviewed [46][47][48]…”

Section: Introductionmentioning

confidence: 99%

Stability-Indicating Photochemical Method for the Assay of Thiamine by Spectrophotometry

Ahmad

Abbas

Anwar

et al. 2018

Journal of Spectroscopy

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A stability-indicating photochemical method has been developed for the assay of thiamine (TH) salts in aqueous solution and in fresh and aged vitamin preparations. It is based on the photooxidation of TH by UV irradiation to form thiochrome (TC) in alkaline solution. e TC : TH ratio under controlled conditions of light intensity, temperature, pH, exposure time, and irradiation distance is constant and can be used to determine the concentration of UV irradiated TH solutions. TC, on extraction with isobutanol from the photodegraded solution of TH, has been determined by the UV spectrophotometric method at 370 nm. It exhibits a high intensity of absorption in the UV region that can be used for the assay of even low concentrations of TH. Under optimum conditions, Beer's law is obeyed in the concentration range of 0.20-2.00 mg/100 ml (R 2 � 09998). e limit of detection (LOD) and limit of quantification (LOQ) are 0.0076 and 0.0231 mg/100 ml, respectively. e method has been validated and applied to aqueous solutions and vitamin preparations. e results have statistically been compared with the United States Pharmacopeia liquid chromatography method. It has been found that there is no significant difference between the two methods at 95% confidence level.

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“…B vitamins have been reported to potentiate the antinociceptive and analgesic effects of diclofenac sodium (DC), and several papers have docu-mented their contributions to reduce daily DC dosage and shorten the treatment time [1][2][3]. Through literature, determinations of B-complex vitamins were carried out, by high-performance liquid chromatography (HPLC) [4][5][6][7][8][9][10][11][12][13], spectrophotometry [14][15][16][17], capillary electrophoresis [18][19] and TLC [20]. Benfotiamine (BN) was only determined in pharmaceutical product in combination with cyanocobalamine and thioctic acid by HPLC [21].…”

Section: Introductionmentioning

confidence: 99%

Application of LC and HPTLC-densitometry for the simultaneous determination of two multicomponent mixtures containing pyridoxine hydrochloride and cyanocobalamine

Hadad

Salam

Hameed

2013

Acta Chromatographica

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Summary. Two methods were presented for the simultaneous determination of two multicomponent mixtures containing pyridoxine hydrochloride (B 6 ) and cyanocobalamine (B 12 ) with benfotiamine (BN) [mix. 1], or thiamine nitrate (B 1 ) and diclofenac sodium (DC) [mix. 2]. The first high-performance liquid chromatographic (HPLC) method depends on the use of a cyanopropyl column at a flow rate of 1.5 mL min −1 with a mobile phase consisting of acetonitrile-25 mM ammonium acetate, pH 3.7 (10:90, v/v), for mix. 1 or acetonitrile-25 mM ammonium acetate, pH 5.0 by gradient elution, changing the proportion of the system linearly with a time schedule program, for mix. 2. Quantitation was achieved by UV detection at 220 nm. The second method was based on HPTLC separation followed by densitometric measurement of the spots at 254 nm. The separation was achieved on HPTLC silica gel F 254 plates using chloroform-ethanol-wateracetic acid (5:8:2:0.5, v/v/v/v) for mix. 1 and chloroform-ethanol-water-acetic acid (1.5:8:2:0.5, v/v/v/v) for mix. 2. The proposed methods were successfully applied for the analysis of pharmaceutical formulations containing the two multicomponent combinations.

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A comparison of matrix resolution method, ratio spectra derivative spectrophotometry and HPLC method for the determination of thiamine HCl and pyridoxine HCl in pharmaceutical preparation

Cited by 43 publications

References 10 publications

Simultaneous Spectrophotometric Methods for Estimation of Cefpodoxime Proxetil and Potassium Clavulanate in Tablet Dosage form

Simultaneous Spectrophotometric Methods for Estimation of Cefpodoxime Proxetil and Potassium Clavulanate in Tablet Dosage form

Stability-Indicating Photochemical Method for the Assay of Thiamine by Spectrophotometry

Application of LC and HPTLC-densitometry for the simultaneous determination of two multicomponent mixtures containing pyridoxine hydrochloride and cyanocobalamine

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