Composition of the Essential Oil ofNepeta nuda L. ssp.albiflora (Boiss.) Gams

Abstract

The composition of the essential oil of Neperu nuda L. ssp. ulbipora (Boiss.) Gams (Lamiaceae) was analysed by means of capillary GC and GC-MS. The main components, accounting for 75.1% of the oil, were 4aa,7a,7aa-nepetalactone (37.5%) and 4aa,7~,7afhepetalactone (37.6%). The sesquiterpene fraction amounted to 14.2% and was dominated by caryophyllene oxide (4.4%). EXPERIMENTAL Plant MaterialThe leaves and flowering tops of Nepeta nuda ssp. albiflora were collected in July 1994 near &el in southern Turkey. A voucher specimen was deposited in the Herbarium of Ankara University, Faculty of Pharmacy (AEF 19147). Isolation of the Essential OilThe essential oil was obtained by hydrodistillation for 3 h in a Clevenger-type apparatus. The yield of the oil was 1.1%. based on the dry weight of the sample.Gas Chromatography GC analyses were performed using a Shimadzu GC 14A gas chromatograph with an OV-1 fused silica column (25 m x 0.25 mm i.d., film thickness 0.24 pm) and SE-52 fused silica column (50 m x 0.32 mm i.d., f i l m thickness 0.29 pm). Oven temperature was held at 40°C for 4 min, programmed to 5OoC at a rate of 2"C/min, then held at 50°C for 10 min and programmed to 270°C at a rate of 2"Umin. Injector and detector (FID) temperatures were kept at 250°C. Split ratio was 1:30. Gas Chromatography-Mass Spectrometry GC-MS analysis were camed out with an HP 5890-VG Zabspec GC-MS system on an OV-1 fused silica column (30 m x 0.32 mm id.). Oven temperature was as above; transfer line temperature 220°C; ion source temperature 200°C; carrier gas helium; splitting ratio 1:40; ionization energy 70 eV; trap current 200 PA; scan range 50-700 amu; scan time 2 s.

Phytotoxic effects of essential oils ofNepeta curvifloraBoiss. andNepeta nudaL. subsp.albifloragrowing wild in Lebanon

“…In fact, the essential oil from N. nuda L. ssp. albiflora harvested in southern Turkey is characterized by high content of three nepetalactones: (Z,E)-nepetalactone (37.5%), (E,Z)-nepetalactone (37.6%) and (Z,Z)-nepetalactone (2.1%) (Kokdil et al 1996). The oil from the same plant collected in another region of Turkey contained great percentages of nerolidol (37.1%), 1,8-cineole (29.1%) and spathulenol (14.4%) (Sarer and Konuklugil 1996).…”

“…albiflora investigated in this study is different from those reported for other N. nuda ssp. albiflora oils as reported in the literature (Kokdil et al 1996;Sarer and Konuklugil 1996). In fact, the essential oil from N. nuda L. ssp.…”

“…N. nuda ssp. albiflora is characterized by the presence of monoterpene nepetalactones, steroids, terpenes, iridoids (Takeda et al 1995(Takeda et al , 1996Kokdil et al 1999) and essential oils (Kokdil et al 1996;Sarer and Konuklugil 1996). Recently, N. pannonica and N. juncea were reported for their phytotoxic properties (Kobaisy et al 2005;Hussain et al 2009).…”

Fatty acid composition of seeds of some species ofNepetaL.

“…Many studies have been performed on the essential oil composition of species of Nepeta and nepetalactones, which resulted in the isolation of some steroidal compounds [7][8][9][10][11][12]. The main essential oil components of the species of Nepeta were reported as 1,8-cineole, citronellol, pregeijerene, and nepetalactones [7][8][9].…”

“…The main essential oil components of the species of Nepeta were reported as 1,8-cineole, citronellol, pregeijerene, and nepetalactones [7][8][9]. However, there is no reported study on the fatty acid composition of seeds of species of Nepeta in the literature.…”