Summary
An improved medium for the isolation of Mycoplasma species from contaminated cell cultures is reported. A modification of the mycoplasma detection method using the uridine/uracil uptake ratio method is described. Results obtained using this method with mycoplasma contaminated cell cultures and with contaminated cell cultures treated with the antibiotic Lincomycin are presented.
Chromosome analysis of three lymphoblast cell lines from New Guinea Burkitt lymphoma (QIMR‐GOR, QIMR‐AMB/1 and AMB/2), three lymphoblast cell lines from infectious mononucleosis (QIMR‐DAR, QIMR‐STE and QIMR‐GOE) and two lymphoblast cell lines from leukaemia (QIMR‐WIL and QIMR‐FIN) showed abnormalities associated with Group A, C, and G chromosomes. Each cell line contained diploid and near diploid cells. Some of the cell lines also contained pseudodiploid cells. Extra Group C or Group G chromosomes were found in a proportion of cells from some of the cell lines. Abnormal Group A chromosomes were also found. However, no one abnormality could confidently be assigned to a specific source of origin of the cell line studied.
Summary
Several antibiotics were tested for their toxicity on three cell types—the heteroploid H.Ep.2 cell line, primary cynomolgus monkey kidney cells, and a diploid human embryonic skin line. The elimination of specific contaminants by particular antibiotics is discussed, and suitable concentrations of the antibiotics for use in tissue cultures are recommended. Kanamycin, neomycin, fungizone, chloramphenicol, viomycin and polymyxin B are suitable antibiotics for the elimination of the micro‐organisms most frequently encountered as contaminants of tissue cultures.
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