Growth of Diploid and Heteroploid Tissue Cultures in the Presence of Some Antibiotics

Abstract

Summary Several antibiotics were tested for their toxicity on three cell types—the heteroploid H.Ep.2 cell line, primary cynomolgus monkey kidney cells, and a diploid human embryonic skin line. The elimination of specific contaminants by particular antibiotics is discussed, and suitable concentrations of the antibiotics for use in tissue cultures are recommended. Kanamycin, neomycin, fungizone, chloramphenicol, viomycin and polymyxin B are suitable antibiotics for the elimination of the micro‐organisms most frequently encountered as contaminants of tissue cultures.