Chromosome studies on cultured lymphoblast cell lines from cases of new guinea burkitt lymphoma, myeloblastic and lymphoblastic leukaemia and infectious mononucleosis

Tomkins, GA

doi:10.1002/ijc.2910030513

Cited by 13 publications

(2 citation statements)

References 18 publications

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“…A similar abnormality was also found in lymphoid lines (LCL) from other sources, however (Kohn et al, 1968;Pope et al, 1968;Chessin et al, 1968;Miles et al, 1968;Macek and Benyesh-Melnick, 1969). In other studies with EBV-carrying LCL cells this marker was only found in a minority of the lines and in a small proportion of the metaphases (Tomkins, 1968;Saksela and PontCn, 1968;Huang et al, 1970;Whang-Peng et a/., 1970).…”

mentioning

confidence: 83%

Characteristic chromosomal abnormalities in biopsies and lymphoid‐cell lines from patients with burkitt and non‐burkitt lymphomas

Zech

Haglund

Nilsson

et al. 1976

Intl Journal of Cancer

795

248

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The karyotypes of cells from 10 Burkitt lymphoma (BL) biopsies, eight cell lines established from BL and nine cell lines from non-BL sources were studied by chromosome banding techniques. With the exception of the BL-derived cell lines BJAB, GC-BJAB, Maku and U-8691 all biopsies and lines of Burkitt origin contained an extra band at the distal region of the long arm of one chromosome 14. An extra band on chromosome 14 was also found in cells of one non-BL biopsy, in cells from a lymphosarcoma-derived cell line and in a long-established cell line derived from the pleural exudate of a patient with Hodgkin's disease. A distal region at the long arm of one chromosome 8 was missing in all metaphase figures of good technical quality in the same material. The size, morphology and stain-ability of the missing region corresponded fairly well to the extra region at chromosome 14. We therefore suggest that the chromosome 14 marker represents a translocation between chromosomes 8 and 14,t (8q-; 14q+). The translocation was present neither in lymphocytes of the peripheral blood of five Burkitt patients nor in five lymphoblastoid cell lines of non-BL origin. Trisomy 7 was found in two of the 10 BL biopsies, in two BL-derived cell lines, in one non-BL biopsy, in two lymphosarcoma-derived cell lines and in one cell line derived from a patient with Hodgkin's disease.

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mentioning

confidence: 83%

Characteristic chromosomal abnormalities in biopsies and lymphoid‐cell lines from patients with burkitt and non‐burkitt lymphomas

Zech

Haglund

Nilsson

et al. 1976

Intl Journal of Cancer

795

248

View full text Add to dashboard Cite

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“…Previous cytogenetic studies on infectious mononucleosis (IM) have mainly been undertaken in long-term lymphocyte cultures (Tomkins 1968), which are comparatively easy to establish from subjects with previous exposure t o Epstein-Barr virus (EBV), now recognised as the causative agent in IM (Klein 1973). Direct chromosome analyses, on the other hand, have been hampered by the reduced susceptibility of IM lymphocytes to the mitogen phytohaemagglutinin (Rassiga Pidot et al 1973).…”

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confidence: 99%

Cytogenetic study of 10 cases of infectious mononucleosis

1977

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Cytogenetic analysis of 10 cases of infectious mononucleosis has revealed increased damage in the form of chromosome breakage and aberrations typical of viral infection, but in addition a few cells were consistently found to harbour a deleted number 22, similar to the Philadelphia chromosome. The presence of a plasma inhibitor of lymphocyte response to the mitogen phytohaemagglutinin is suggested. This inhibitor appears to have a reversible action on the patient's own lymphocytes but no inhibitory effect on control lymphocytes.

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Chromosomal variability in ten cloned sublines of a newly established Burkitt's lymphoma cell line

Ikeuchi

Minowada

Sandberg

1971

Cancer

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Cytogenetic observations were made in a newly established Burkitt's lymphoma cell line (B46M) and its 10 cloned sublines. The parental cell line had a fairly sharp mode of 46 chromosomes with a normal male karyotype, except for the presence of two marker chromosomes (a B‐group chromosome with an elongated short arm and a G‐group chromosome with a partially deleted long arm). In spite of the high karyologic homogeneity (70%) in the parental cell, population, it was found that only 2 clones contained the same chromosome complement as that of the parental cells. Five clones had 1 or 2 additional modes at 45 or 47, besides 46, in which most cells maintained the parental karyotype. In these cases, there was a preferential loss and addition of G and C‐X‐group chromosomes, respectively. Each of the remaining 3 clones showed a common chromosomal abnormality in all the cells examined—a partial monosomy for the long arm of E18, an absence of the Y‐chromosome, and a possible isochromosome of one of D13–15. The evidence for the production of both γ and μ heavy chains and χ light chain in all the clones, as well as in the parental cell line, suggests that the genes controlling the immunoglobulin synthesis were not located on the parts of the 3 chromosomes described. The mechanism by which the variant clones were developed from the parental cells was discussed, with some remarks on the possible usefulness of the system employed in the present study in obtaining mutant clones necessary for studying human genetics in vitro.

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Chromosome studies on cultured lymphoblast cell lines from cases of new guinea burkitt lymphoma, myeloblastic and lymphoblastic leukaemia and infectious mononucleosis

Cited by 13 publications

References 18 publications

Characteristic chromosomal abnormalities in biopsies and lymphoid‐cell lines from patients with burkitt and non‐burkitt lymphomas

Characteristic chromosomal abnormalities in biopsies and lymphoid‐cell lines from patients with burkitt and non‐burkitt lymphomas

Cytogenetic study of 10 cases of infectious mononucleosis

Chromosomal variability in ten cloned sublines of a newly established Burkitt's lymphoma cell line

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