Rapid Detection and Isolation of Mycoplasmas From Cell Cultures

Tomkins, GA; MacGregor, A.; Pye, David; Atkinson, Mary

doi:10.1038/icb.1975.28

Cited by 20 publications

(2 citation statements)

References 12 publications

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“…Crow cells were grown as a monodisperse suspension culture in Eagle's Minimum Essential Medium (MEM) with 10% foetal calf serum (both from Flow Laboratories, Stanmore, N.S.W.). The cells were subcultured twice weekly (usually 1/10 each time) and were monitored for mycoplasma contamination by a dual label incorporation technique (14).…”

Section: Methodsmentioning

confidence: 99%

Effect of several inhibitors of enzymatic DNA methylation on thein vivomethylation of different classes of DNA sequences in a cultured human cell line

Woodcock¹,

Adams²,

Allan³

et al. 1983

Nucl Acids Res

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We have previously demonstrated that, while most enzymatic formation of 5-methylcytosine in the DNA of mammalian cells occurs very shortly after strand synthesis, there is also a minor fraction of methylation which occurs in some DNA sequences up to at least several hours after strand synthesis. Using a human cell line, we have examined the effects on these two classes of enzymatic DNA methylation of several compounds which have been reported to be inhibitors of methylation reactions. We have found that cycloleucine, ethionine, and 5'-deoxy-5'-methylthioadenosine (MTA) are all effective as inhibitors of enzymatic DNA methylation, but that there is no differential effect between the delayed and non-delayed methylation reactions. Tubericidin (7-deaza-adenosine) plus homocysteine inhibited delayed DNA methylation much more than non-delayed methylation (by up to 4 times). By contrast, 5-azacytidine produced a higher level of inhibition of DNA methylation at sites in the DNA in which the methylation occurred very shortly after strand synthesis. Also 5-azacytidine was by far the most potent inhibitor of DNA methylation of the compounds tested. S-Adenosyl-homocysteine and caffeine were found to have no effect on DNA methylation. These results are discussed in relation to the number and specificity of DNA methylases in these cells and to the cellular functions of those DNA sequences in which methylation is delayed for some hours after strand synthesis.

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Section: Methodsmentioning

confidence: 99%

Effect of several inhibitors of enzymatic DNA methylation on thein vivomethylation of different classes of DNA sequences in a cultured human cell line

Woodcock¹,

Adams²,

Allan³

et al. 1983

Nucl Acids Res

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show abstract

“…Cultures were maintained in Eagle's basal medium buffered with 20 mmol/1 Hepes (4-(2hydroxyethyl)-1-piperazine-ethanesulphonic acid) and supplemented with 10 % fetal calf serum. Cell lines were demonstrated to be free of mycoplasma contamination by the method of Tomkins et al, (1975). Before collagen studies, stocks of each cell line were grown in Dulbecco's modification of Eagle's medium buffered with 20 mmol/1 Hepes and supplemented with 10 % (v/v) fetal calf serum.…”

Section: Cell Culturementioning

confidence: 99%

Normal hydroxylation of proline in collagen synthesized by skin fibroblasts from a patient with prolidase deficiency

Royce

Danks

1981

J of Inher Metab Disea

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A hemopoietic cell line dependent upon a factor in pokeweed mitogen‐stimulated spleen cell conditioning medium

Hasthorpe

1980

Journal Cellular Physiology

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A continuously growing cell line (FMP1.1) has been isolated, which is dependent upon a factor in PWCM for both growth and survival. FMP1.1 appears to be a mast cell, since IgE receptors are present and their granules react specifically with a mast cell granule stain. The factor in PWCM may be a glycoprotein and has biochemical properties in common with PWCM factors, which stimulate at least four other lineages of hemopoietic colony-forming cell. This line provides a pure population of cells as a model for studying molecular events of hemopoietic precursor cell growth and differentiation.

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Rapid Detection and Isolation of Mycoplasmas From Cell Cultures

Cited by 20 publications

References 12 publications

Effect of several inhibitors of enzymatic DNA methylation on thein vivomethylation of different classes of DNA sequences in a cultured human cell line

Effect of several inhibitors of enzymatic DNA methylation on thein vivomethylation of different classes of DNA sequences in a cultured human cell line

Normal hydroxylation of proline in collagen synthesized by skin fibroblasts from a patient with prolidase deficiency

A hemopoietic cell line dependent upon a factor in pokeweed mitogen‐stimulated spleen cell conditioning medium

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