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1. Intraperitoneal injection of beta-sitosterol (5mg./rat/day for 25 days) into 1-year-old male Wistar rats fed on a low-fat diet supplemented with 10% of coconut oil resulted in a lowering of cholesterol and lipid concentrations in the tissues. 2. beta-Sitosterol increased the rate of biosynthesis of cholesterol and lipids in the tissues, but to an even greater extent enhanced their oxidative degradation. 3. The present results are similar to those previously obtained on a low-fat diet, indicating that the presence of fat had no marked effect on the action of beta-sitosterol.

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The biohydrogenation of unsaturated fatty acids and the preferential absorption of unsaturated fatty acids over long chain saturated fatty acids from the gut have been investigated to find the origin of the high saturated fatty acid content of the facal lipids of rats fed soybean oil. Label from dietary (1‐14C)‐linoleic acid was recovered in the saturated and monounsaturated fatty acids of the fecal lipid. However, when (9,10‐3H)‐stearic acid and (1‐14C)‐linoleic acid were fed together, the isotope ratio (3H/14C) of the fecal lipid was 1.9 times that of the diet. It is concluded that both processes occur.