Juglone (5-hydroxy-1,4-napthoquinone) is a chemical compound released by walnut trees that can be toxic for surrounding plant species. In the present study, juglone was identified in leaves and green husk in five walnut cultivars: 'Germisara' , 'Jupanesti' , 'Franquette' , 'Vina' , 'Valcor' by using high performance liquid chromatography (HPLC-RP). Juglone was found predominant in green husk (average value of cultivars is about 31.308 mg/100 g). Significant differences in contents of identified juglone were observed among cultivars that ranged from 20.56 to 42.78 mg/100g for green husk, and 5.42 to 22.82 mg/100 g for leaves. It was also found that walnut green husk and leaves represent the most important source of walnut phenolics.

Fruits of two jujube (Ziziphus jujuba Mill.) cultivars were collected at four stages of ripening and analyzed in terms of chemical compounds. The cultivars were grown under the climatic conditions of the Sub-Carpathian area Ramnicu-Valcea in Romania (45°6′17″N, 24°22′32″E). Total phenols content, total flavonoids content, organic acids from jujube fruits and related antioxidant activities were investigated during the ripening stages. The antioxidant activity of alcoholic extracts was investigated by the 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. Total phenolic content was determined by using the Folin-Ciocalteu assay, while determination of flavonoids was done by aluminium nitrate colorimetric method, and organic acids were separated by reversed phase chromatography. The results obtained have indicated significant differences (p < 0.05) found in bioactive compounds and antioxidant activity, depending on cultivars and ripening stage. Total phenolic content ranged from 1634.44 to 472.38 mg GAE/100g in methanolic extracts of the two varieties studied, while total flavonoids content ranged from 19.9 to 48.5 mg QE/100 g. As regards the influence of ripening stage, the results show that the highest antioxidant activity was recorded in the stage of white maturity. The impact of maturity stage was significant on organic acid distribution of jujube extracts, and ascorbic acid is the major organic acid found in jujube fruits, followed by malic acid and lactic acid. Jujube fruits are rich in bioactive compounds and can be a complement in human's healthy eating.

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Conservation of fruit tree biodiversity is important for the mankind according to the Convention on Biological Diversity. In Romania, due to favorable environmental conditions, numerous genetic resources of plum, apple, walnut, hazelnut, sweet chestnut and other fruit crops are present. Identification, evaluation and conservation of fruit genetic resources activities were launched in 1970' in order to limit the loss of the biodiversity due to erosion and genetic vulnerability.

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