No abstract

This study was undertaken to evaluate the effectiveness of L-ascorbic acid (AA) in alleviating the toxicity of aflatoxin B1 (AFB1) in male New-Zealand white rabbits. Five rabbits (6 months of age and mean body weight 3.12 kg) per group were assigned to 1 of 6 treatment groups: 0 mg AA and 0 mg AFB1/kg BW (control); 20 mg AA/kg BW; 15 microg AFB1/kg BW; 15 microg AFB1 plus 20 mg AA/kg BW; 30 pg AFB1/kg BW; 30 pg AFB1 plus 20 mg AA/kg BW. Rabbits were orally administered their respective doses every other day for 9 weeks, followed by a 9-week recovery period where all drugs were withdrawn. Evaluations were made for hemato-biochemical parameters and enzymatic activities. Results showed that AFB1 significantly (p< 0.05) decreased hemoglobin (Hb), total erythrocytic count (TEC) and packed cell volume (PCV), in a dose-dependent manner, and these effects were continued during the recovery period. Ascorbic acid caused an increase in these parameters, and alleviated the negative effect of AFB1 during the treatment period. Additionally, serum concentrations of total protein, albumin and glucose were significantly (P < 0.05) declined by treatment with the high dose of aflatoxin and these effects were continued during the recovery period. Ascorbic acid caused non-significant increases in these parameters and alleviated the harmful effect of AFB1. On the other hand, aflatoxin treatment caused significant increases (P < 0.05) in the activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (AlP) during the treatment period in a dose dependent manner, and this effect was continued during the recovery period, especially with the high dose. Also, treatment with the high dose of aflatoxin caused significant increases (P<0.05) in cholesterol and total bilirubin. Ascorbic acid caused significant decreases in these parameters and alleviated the harmful effects of AFB1. Whereas, Total leukocyte count (TLC), urea and creatinine were not significantly affected by aflatoxin-treatment. Generally, it is interesting feature that the treatment with AA alone had no negative effects on most of the previous parameters. Also, the presence of AA could diminished the adverse effects of AFB1 on most of hematological and biochemical values, and enzymatic activities in rabbits.

No abstract

Gonadotropin-releasing hormone (GnRH)–loaded chitosan nanoparticles (GnRH–ChNPs) were used at different doses and routes of administration to induce ovulation in rabbits as an attempt to improve artificial insemination (AI) procedures and outcomes. In this study, the characteristics (size, polydispersity, loading efficiency, and zeta-potential) of GnRH–ChNPs and the GnRH release pattern were determined in an in vitro study. A first in vivo study assessed the pituitary and ovarian response to different GnRH–ChNPs doses and routes of administration (two i.m. doses, Group HM = 0.4 µg and Group QM = 0.2 µg, and two intravaginal doses, Group HV = 4 µg and Group QV = 2 µg) against a control group (C) receiving bare GnRH (0.8 µg). The HM, QM, and HV treatments induced an earlier LH-surge (90 min) than that observed in group C (120 min), whilst the QV treatment failed to induce such LH surge. The number of ovulation points was similar among treatments, except for the QV treatment (no ovulation points). A second in vivo study was consequently developed to determine the hormonal (progesterone, P4, and estradiol, E2) profile and pregnancy outcomes of both HM and HV treatments against group C. The treatment HM, but not the treatment HV, showed adequate P4 and E2 concentrations, conception and parturition rates, litter size, litter weight, and viability rate at birth. Overall, the use of GnRH–ChNPs allows for a reduction in the conventional intramuscular GnRH dose to half without compromising fertility. However, the addition of GnRH–ChNPs to semen extenders, although successfully inducing ovulation, has negative impacts on fertility. Thus, more studies are needed to explore this point and allow further adjustments.

No abstract

The present study was undertaken to determine the effect of chronic treatment with two sublethal doses of Dimethoate (organo-phosphorus) or Deltamethrin (pyrethroid) on body weight and semen characteristics in adult male rabbits. Pesticide treatment resulted in a decline in body weight, libido, ejaculate volume, sperm concentration and semen initial fructose; and an increase in abnormal and dead sperm and methylene blue reduction time. In this regard Dimethoate showed greater effects than Deltamethrin. The hazardous effect of these pesticides on semen quality continued during the post-treatment period, and was dose-dependent. This deleterious effect on sperm formation together with the decline in libido suggest a decrease in testosterone secretion by pesticide treatment.

Water requirements and metabolism were studied in eight each of Barki and Rahmani sheep and eight Baladi goats. The animals were individually housed in the shade in semi-open pens that were located in a non-desert area. The experiments were carried out during spring, summer and winter seasons, and each experiment lasted for 3 weeks during each season. After a preliminary period of 1 week, an 8-day period was then followed during which body weight, daily feed intake and daily drinking water were recorded. Rectal temperature and respiration rate were also recorded in the early morning and late afternoon. Four animals from each group were then weighed and injected intravenously with tritiated water, TOH (001 mCi/kg body weight) and blood samples were collected during a 4-day period to estimate total body water (TBW) and water turnover rate (WTR).In all seasons studied, the Rahmani sheep had higher TOH disappearance rate (^-values) and WTR and consequently shorter biological half-time (t0 than the Barki. Moreover, the Barki sheep showed less thermal discomfort than the Rahmani particularly during the summer. Although the Rahmani sheep ingested more water than the Barki sheep during the summer, the Rahmani sheep had a lower percentage of TBW and their calculated water loss revealed that they were in negative water balance during this season. Accordingly, the Barki sheep seem to perform better than the Rahmani in newlyreclaimed desert areas. Baladi goats appear to be more adapted to unfavourable environmental conditions than sheep. This is based upon the findings that they had lower ^-values, lower WTR, longer ti and higher TBW, than the two breeds of sheep.TNTRODnrTTON w e " a s * ne phy s '°'°g' ca ' responses (rectal temperature and respiration rate) of Barki and Rahmani Determination of daily water exchange between sheep and of Baladi goats during spring, summer animals and their environment is important for the and winter seasons, estimation of their water requirements and the evaluation of their adaptability and productivity. In MATERIALS AND METHODS ruminants daily water turnover rate is influenced by breed, environmental temperature, pregnancy, lacta-The present study was carried out on 16 ewes tion, age, sex and availability of water in the (including eight desert-adapted Barki and eight environment (MacFarlane & Howard, 1972; Mackie, temperate-evolved Rahmani) and eight Baladi 1977;Degen & Young, 1981;Khan, 1983). Intro-goats belonging to the University of Alexandria duction of sheep and goats to the newly-reclaimed Experimental Station. The experiments were indesert areas of Egypt necessitates the determination itiated in April 1983 and were terminated in February of their daily water turnover rate, in order to ensure 1984. All animals were dry, non-pregnant and were adequate water supply and to choose the species or 1-2 years of age at the beginning of the experiment, the breed which is better adapted to arid and semi-The animals ^were individually housed in semi-open arid conditions. Such animals should b...

The in vivo effect of dimethoate and deltamethrin on body and organ weights, serum proteins and on plasma acetylcholinesterase (AChE), aromatic esterase and ATPase were examined in growing male rabbits throughout five months period. Both compounds had no significant effect on body weight; however, adrenal, testis & pituitary weights decreased (P less than 0.01); the liver and spleen weights increased (P less than 0.01) in a dose dependent manner. Serum total proteins and globulin decreased (P less than 0.01) in a dose dependent trend, while serum albumin was not greatly affected. AChE activity was increased (P less than 0.01) after 1 month of treatment with the two doses of dimethoate and deltamethrin; thereafter, AChE activity showed 40% inhibition of the control level. The activity of aromatic esterase increased markedly after the first month, then declined gradually until the fifth month. High dose of dimethoate markedly inhibited this enzyme particularly after the 5th month of treatment. Both doses of deltamethrin increased ATPase activity after the first month of treatment, then the ATPase activity was normal. Dimethoate inhibited ATPase particularly at the end of treatment in a dose dependent manner.