The detection of microRNA (miRNA) in human serum has great significance for cancer prevention. Herein, a novel self-powered biosensing platform is developed, which effectively integrates an enzymatic biofuel cell (EBFC)-based self-powered biosensor with a matching capacitor for miRNA detection. A catalytic hairpin assembly and hybrid chain reaction are used to improve the analytical performance of EBFC. Furthermore, the matching capacitor is selected as an auxiliary signal amplifying device, and graphdiyne is applied as substrate material for EBFC. The results confirm that the developed method obviously increases the output current of EBFC, and the sensitivity can reach 2.75 μA/pM, which is 786% of pure EBFC. MiRNA can be detected in an expanded linear range of 0.1−100000 fM with a detection limit of 0.034 fM (S/N = 3). It can offer a selective and sensitive platform for nucleotide sequence detection with great potential in clinical diagnostics.
There has been an explosion of interest in the use of nanomaterials for biosensing applications, especially, carbonaceous nanomaterials are at the forefront of this explosion. Carbon dots (CDs), a new...
A capacitor coupled with enzymatic biofuel cells (EBFCs) can deliver high-power instant output to obtain an amplified detection signal. Herein, matching a capacitor to a self-powered electrochemical biosensor for ultrasensitive determination of microRNA-21 is investigated. The detection system mainly combines a capacitor, EBFCs, and biological amplification technologies. Concretely, EBFCs are integrated into a capacitor-joined circuit, and the capacitor is automatically shorted by a switching regulator to provide an instantaneous current that is rapidly detected with a digital multimeter. A sensitivity of 38.72 μA/pM is achieved with the contribution of the matching capacitor, which is 6.96 times that without a capacitor. Moreover, the redox reaction on the anode leads to high-voltage outputs at low substrate concentrations and generates electrons when the target miRNA triggers a catalytic hairpin assembly cycle, while the release of the [Fe(CN) 6 ] 3− electron acceptor on the cathode is catalyzed to obtain a higher detection signal. As a result, the limit of detection of the developed biosensor is 0.18 fM (S/N = 3) with a linear range of 0.5−10 000 fM, which indicates that the innovative capacitor-matched self-powered biosensor holds great promise for ultrasensitive electrochemical biosensing.
A self-powered microRNAs biosensor with triple signal amplification systems is assembled through integration of three-dimensional DNA walker, enzymatic biofuel cells and capacitor. The DNA walker is designed from an enzyme-free...
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