Construction of an Integrated Device of a Self-Powered Biosensor and Matching Capacitor Based on Graphdiyne and Multiple Signal Amplification: Ultrasensitive Method for MicroRNA Detection

Abstract

The detection of microRNA (miRNA) in human serum has great significance for cancer prevention. Herein, a novel self-powered biosensing platform is developed, which effectively integrates an enzymatic biofuel cell (EBFC)-based self-powered biosensor with a matching capacitor for miRNA detection. A catalytic hairpin assembly and hybrid chain reaction are used to improve the analytical performance of EBFC. Furthermore, the matching capacitor is selected as an auxiliary signal amplifying device, and graphdiyne is applied as substrate material for EBFC. The results confirm that the developed method obviously increases the output current of EBFC, and the sensitivity can reach 2.75 μA/pM, which is 786% of pure EBFC. MiRNA can be detected in an expanded linear range of 0.1−100000 fM with a detection limit of 0.034 fM (S/N = 3). It can offer a selective and sensitive platform for nucleotide sequence detection with great potential in clinical diagnostics.

Biorecognition antifouling coatings in complex biological fluids: a review of functionalization aspects

“…However, as these sensors do not respond directly to changes at the interface not generating an electric signal, and due to the specific requirements of the electrode materials, the issue of fouling has not been addressed in depth. The electrochemical biosensors are very effective in many applications [216][217][218] but it is recognized that the matrix interference from the highly complex real-world samples, such as blood, affects seriously the interaction and is still a critical issue that needs to be addressed. 214 In summary, only a limited number of functional antifouling coatings were reported as ultra-low or low fouling in complex bodily fluids, typically in blood plasma or blood serum.…”

Electrochemical Sensor for Hydrogen Peroxide Based on Prussian Blue Electrochemically Deposited at the TiO2-ZrO2–Doped Carbon Nanotube Glassy Carbon-Modified Electrode

“…The monitoring of low levels of H 2 O 2 is of great importance for modern medicine, environmental control, and various branches of industry ( Gutiérrez et al, 2011 ; Lv et al, 2020 ; Shu and Tang, 2020 ; Wang et al, 2020 ; Zhang et al, 2020 ; Hou et al, 2021 ; Xu et al, 2021 ). In particular, in biological applications, for example, there is the presence of H 2 O 2 in the human body that is converted into OH radicals with high reactivity; an overproduction of OH and O 2 at cell openings promote cell damage and tissue malfunctioning ( Rhee et al, 2010 ; Grisham, 2013 ).…”

A sensitive electrochemical platform integrated with a 3D graphene aerogel for point-of-care testing for tumor markers

“…In order to improve detection sensitivity, several portable, fast and ultrasensitive selfpowered biosensors were developed for the detection of micro-RNA and L-Cys. [21][22][23][24] What's more, various nanomaterials have been modified on electrode surfaces. 25,26 For instance, Li et al used a cuboid silver-modified paper working electrode as the sensor platform and coated porous nanosilver chitosan with different metal ions as the label.…”