Integration of a capacitor to a 3-D DNA walker and a biofuel cell-based self-powered system for ultrasensitive bioassays of microRNAs

Abstract

A self-powered microRNAs biosensor with triple signal amplification systems is assembled through integration of three-dimensional DNA walker, enzymatic biofuel cells and capacitor. The DNA walker is designed from an enzyme-free...

Recent advances in biological detection with rolling circle amplification: design strategy, biosensing mechanism, and practical applications

“…The combination of RCA and various enzyme-free reactions also provides an attractive way in which DNAzyme-assisted and self-assembly methods such as HCR and CHA are frequently reported. [95][96][97] Hu et al constructed an ultrasensitive platform for the detection of Pb 2+ by combining RCA, HCR and DNAzyme. 98 Firstly, the target Pb 2+ was specifically recognized by DNAzyme, hence triggering the enzymatic cleavage of the substrate and the release of Pb 2+ and DNAzyme probe.…”

Biorecognition antifouling coatings in complex biological fluids: a review of functionalization aspects

“…However, as these sensors do not respond directly to changes at the interface not generating an electric signal, and due to the specific requirements of the electrode materials, the issue of fouling has not been addressed in depth. The electrochemical biosensors are very effective in many applications [216][217][218] but it is recognized that the matrix interference from the highly complex real-world samples, such as blood, affects seriously the interaction and is still a critical issue that needs to be addressed. 214 In summary, only a limited number of functional antifouling coatings were reported as ultra-low or low fouling in complex bodily fluids, typically in blood plasma or blood serum.…”

Nucleic acid isothermal amplification-based soft nanoarchitectonics as an emerging electrochemical biosensing platform

“…38 To further achieve higher reaction efficiency, the captured hairpin DNA could also be modified on nanomaterials, such as gold nanomaterials (AuNPs or gold nanorods) and magnetic nanobeads to construct 3D DNA nanomachine-mediated CHA 39 or without using nanomaterials, a transduction hairpin (THP) DNA with stem-loop structure is used to form three-leg DNA nanomachine. 40 Benefitting from the improved reaction rate and the conversion efficiency, 3D DNA nanomachine-mediated CHA realized a detection limit of 0.27 fM miRNA-155 on gold electrode, 40 0.139 fM miRNA-21 on magnetic glassy carbon electrode (MGCE), 39 0.17 fM miRNA-21 in enzymatic biofuel cells (EBFCs) 41 and 0.32 aM miRNA-21 on magnetic AuNFs/ITO electrodes. Further combining loop-mediated isothermal amplification (LAMP) with CHA, which takes full advantage of the ultrasensitive feature of nucleic acid amplification, an extremely small amount of gene targets (down to 2 copies per μL) was analyzed (Fig.…”