Recent advances in biological detection with rolling circle amplification: design strategy, biosensing mechanism, and practical applications

Gao, Yongping; Huang, Ke‐Jing; Wang, Futing; Hou, Yachen; Xu, Jing; Li, Guoqiang

doi:10.1039/d2an00556e

Cited by 26 publications

(10 citation statements)

References 210 publications

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“…However, it should be noted that the NASBA amplification efficiency is low when the nucleotide sequence is >250 or <120 bp ( Table II ) ( 77 ). In addition, due to the low temperature requirements of the NASBA reaction, it is easy for primer dimerization and non-specific amplification to occur, which markedly increases the false-positive rate ( 78 ).…”

Section: Isothermal Amplificationmentioning

confidence: 99%

Advances in the application of molecular diagnostic techniques for the detection of infectious disease pathogens (Review)

et al. 2023

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Infectious diseases are a major global cause of morbidity and mortality, seriously affecting public health and socioeconomic stability. Since infectious diseases can be caused by a wide variety of pathogens with similar clinical manifestations and symptoms that are difficult to accurately distinguish, selecting the appropriate diagnostic techniques for the rapid identification of pathogens is crucial for clinical disease diagnosis and public health management. However, traditional diagnostic techniques have low detection rates, long detection times and limited automation, which means that they do not meet the requirements for rapid diagnosis. Recent years have seen continuous developments in molecular detection technology, which has a higher sensitivity and specificity, shorter detection time and increased automation, and performs an important role in the early and rapid detection of infectious disease pathogens. The present study summarizes recent progress in molecular diagnostic technologies such as PCR, isothermal amplification, gene chips and high-throughput sequencing for the detection of infectious disease pathogens, and compares the technical principles, advantages and disadvantages, applicability and costs of these diagnostic techniques.

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Section: Isothermal Amplificationmentioning

confidence: 99%

Advances in the application of molecular diagnostic techniques for the detection of infectious disease pathogens (Review)

et al. 2023

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“…In the realm of bioanalysis, signal amplification is a key measure to achieve high sensitivity detection of target analytes. Various strategies, such as catalytic hairpin assembly (CHA), 67 hybridization chain reaction (HCR), 68,69 roll circle amplification (RCA), 70 entropy-driven amplification (EDA) reaction, 71,72 clustered regularly interspaced short palindromic repeats and its associated protein (CRISPR-Cas) system, [73][74][75] have been developed to enhance bio-amplification signals and achieve satisfactory sensing performance. Despite the 3D walker being a viable amplifier for analytical applications, it falls short in the detection of low-abundance analytes.…”

Section: D Walkers Coupled With Diverse Isothermal Amplification Tech...mentioning

confidence: 99%

The rate-limiting procedure of 3D DNA walkers and their applications in tandem technology

Chen

et al. 2023

Chem. Commun.

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“…Isothermal amplification combined with DNAzyme walker has the potential to mitigate the challenge due to the ease in integration with additional components and high amplification efficiency under a constant temperature. , In particular, rolling circle amplification (RCA) is one of the most attractive techniques for universal low-abundance molecular detection benefiting from its amplified products containing thousands of periodic repeat sequences. − For example, Ju et al presented a cascade DNA reaction by hybridizing RCA-produced nanowire with hairpin probes, which could be used for the detection of different mRNAs . The Xu and Huang group developed a dual-mode RCA biosensing platform, achieving electrochemical and colorimetric detection of microRNA with detection limits of 0.15 and 33 fM .…”

Section: Introductionmentioning

confidence: 99%

Universal Detection and Imaging for Multiple Targets by Coupling Target-Primed Nicking-Enhanced Rolling Circle Amplification with Self-Powered DNAzyme Walker

Zhang

et al. 2023

Anal. Chem.

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Although promising in monitoring low-abundance analytes, most of the DNAzyme walker is only responsive to a specific target. Herein, a universal, ready-to-use platform is developed by coupling nicking-enhanced rolling circle amplification and a self-powered DNAzyme walker (NERSD). It addressed the issues that DNAzyme strands need to be specifically designed for different biosensing system, allowing highly sensitive analysis of various targets with the same DNAzyme walker components. It is also specific owing to target-dependent ligation of the padlock probe and precise cleavage of a substrate by a DNAzyme strand. As typically demonstrated, the strategy has an equivalent capacity with the qRT-PCR kit in distinguishing plasma miR-21 levels of breast cancer patients from normal subjects and is able to differentiate intracellular miR-21 and ATP levels by confocal imaging. The approach characteristic of programmability, flexibility, and generality indicated the potential in all kinds of biosensing and imaging platform.

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Recent advances in biological detection with rolling circle amplification: design strategy, biosensing mechanism, and practical applications

Abstract: Rolling circle amplification (RCA) is a simple and isothermal DNA amplification technique that is used to generate thousands of repeating DNA sequences using circular templates under the catalysis of DNA...

Cited by 26 publications

References 210 publications

Advances in the application of molecular diagnostic techniques for the detection of infectious disease pathogens (Review)

Advances in the application of molecular diagnostic techniques for the detection of infectious disease pathogens (Review)

The rate-limiting procedure of 3D DNA walkers and their applications in tandem technology

Universal Detection and Imaging for Multiple Targets by Coupling Target-Primed Nicking-Enhanced Rolling Circle Amplification with Self-Powered DNAzyme Walker

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