Boosting performance of self-powered biosensing device with high-energy enzyme biofuel cells and cruciform DNA

Wang, Futing; Wang, Yihan; Xu, Jing; Huang, Ke-Jing; Liu, Zhenhua; Lu, Yuele; Wang, Shuyu; Han, Zhaobin

doi:10.1016/j.nanoen.2019.104310

Cited by 65 publications

(20 citation statements)

References 51 publications

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“…In addition, Wang et al prepared a self-powered miRNA electrochemical biosensor using sulfur-selenium codoped graphene/AuNPs as electrode modification materials. [82] The obtained biosensor showed a detection limit of 1.5 × 10 À 16 M with a linear range from 0.5 to 10000 fM, which had a great potential in clinical application. As above-mentioned, the graphene electrode combined with AuNPs has better chemical conductivity, so its sensitivity is better for miRNA detection.…”

Section: Graphene For Microrna Electrochemical Biosensingmentioning

confidence: 85%

Recent Applications of Carbon Nanomaterials for microRNA Electrochemical Sensing

Wang

Wen

Yan

2020

Chemistry An Asian Journal

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MicroRNA (miRNA) is an important tumor marker in the human body, and its early detection has a great influence on the survival rate of patients. Although there are many detection methods for miRNA at present such as northern blotting, real‐time quantitative polymerase chain reaction, microarrays, and others, electrochemical biosensors have the advantages of low detection cost, small instrument size, simple operation, non‐invasive detection and low consumption of reagents and solvents, and thus they play an important role in the early detection of cancer. In addition, with the development of nanotechnology, nano‐biosensors show great potential. The application of various nanomaterials in the development of electrochemical biosensor has greatly improved the detection sensitivity of electrochemical biosensor. Among them, carbon nanomaterials which have unique electrical, optical, physical and chemical properties have attracted increasing attention. In particular, they have a large surface area, good biocompatibility and conductivity. Therefore, carbon nanomaterials combined with electrochemical methods can be used to detect miRNA quickly, easily and sensitively. In this review, we systematically review recent applications of different carbon nanomaterials (carbon nanotubes, graphene and its derivatives, graphitic carbon nitride, carbon dots, graphene quantum dots and other carbon nanomaterials) for miRNA electrochemical detection. In addition, we demonstrate the future prospects of electrochemical biosensors modified by carbon nanomaterials for the detection of miRNAs, and some suggestions for their development in the near future.

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Section: Graphene For Microrna Electrochemical Biosensingmentioning

confidence: 85%

Recent Applications of Carbon Nanomaterials for microRNA Electrochemical Sensing

Wang

Wen

Yan

2020

Chemistry An Asian Journal

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“…More speculatively, the availability of biomolecular receptors may be useful in the development of bioelectronics (i.e., molecular logic gates or switches), achieving a better signal-to-noise ratio. Likewise, it provides the possibility to enable the broad utility of biomolecular recognition in biofuel cells, typically requiring a wide dynamic range for achieving better power efficiencies. , Overall, the strategies presented here may be applicable to all fields relying on biorecognition, and this will be of great potential value to uncovering how their switching thermodynamics have evolved to achieve optimal performance in these fields.…”

Section: Discussionmentioning

confidence: 99%

Re-engineering Electrochemical Aptamer-Based Biosensors to Tune Their Useful Dynamic Range via Distal-Site Mutation and Allosteric Inhibition

Wang

et al. 2020

Anal. Chem.

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Electrochemical aptamer-based (E-AB) sensors, exploiting binding-induced changes in biomolecular conformation, are rapid, specific, and selective and perform well even in a complex matrix, such as directly in whole blood and even in vivo. However, like all sensors employing biomolecular recognitions, E-AB sensors suffer from an inherent limitation of single-site binding, i.e., its fixed dose−response curve. To circumvent this, we employ here distal-site mutation and allosteric inhibition to rationally tune the dynamic range of E-AB sensors, achieving sets of sensors with a significantly varied target affinity (∼3 orders of magnitude). Using their combination, we recreate several approaches to narrow (down to 5-fold) or extend (up to 2000-fold) the dynamic range of biological receptors. The thermodynamic consequences of aptamer−surface interactions are estimated via the freeenergy difference in solution-phase and surface-bound biosensors employing the same aptamer as a recognition element, revealing that an allostery strategy provides a more predictable and efficient means to finely control the target affinity and dynamic range. Such an ability to rationally modulate the affinity of biomolecule receptors would open the door to applications including cancer therapy, bioelectronics, and many other fields employing biomolecule recognition.

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“…78 Nevertheless, the use of co-precipitation (or cocrystallization) agents could be crucial where the MOF crystallization is not triggered by NPs or by enzymes. In addition to magnetic nanoparticles, several multicomponent composite systems based on both functional particles and enzymes have demonstrated to be relevant for application to catalysis, [320][321][322][323] sensing, 322,[324][325][326][327] biomedics, [327][328][329][330] energy production, 322,331,332 and nanomachines/micromotors. 298,333 In the field of heterogeneous catalysis, multicomponent composites are particularly appealing.…”

Section: Figure 20mentioning

confidence: 99%

Metal–Organic Framework-Based Enzyme Biocomposites

et al. 2021

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Due to their efficiency, selectivity, and environmental sustainability, there are significant opportunities for enzymes in chemical synthesis and biotechnology. However, as the three-dimensional active structure of enzymes is predominantly maintained by weaker non-covalent interactions, thermal, pH and chemical stressors can modify or eliminate activity. Metal-organic Frameworks (MOFs), which are extended porous network materials assembled by a bottom-up building block approach from metal-based nodes and organic linkers, can be used to afford protection to enzymes. The self-assembled structures of MOFs can be used to encase an enzyme in a process called encapsulation when the MOF is synthesized in the presence of the biomolecule. Alternatively, enzymes can be infiltrated into mesoporous MOF structures or surface bound via covalent or non-covalent processes. Integration of MOF materials and enzymes in this way affords protection and allows the enzyme to maintain activity in challenge conditions (e.g. denaturing agents, elevated temperature, non-native pH and organic solvents). In addition to forming simple enzyme/MOF biocomposites, other materials can be introduced to the composites to improve recovery or facilitate advanced applications in sensing and fuel cell technology. This review canvasses enzyme protection via encapsulation, pore infiltration and surface adsorption and summarizes strategies to form multi-component composites. Also, given that enzyme/MOF biocomposites straddle materials chemistry and enzymology, this review provides an assessment of the characterization methodologies used for MOF-immobilized enzymes and identifies some key parameters to facilitate development of the field. CONTENTSMOF-based enzyme biocomposite compositions and the concept of encapsulation 3.MOF-based enzyme biocomposites formed via encapsulation 3.1.Templating methods 3.2.One-pot embedding (non-templated) 3.3.Parameters influencing the chemistry of enzyme@MOF biocomposites 3.3.1. Additives 3.3.2.Enzyme suface chemistry 3.3.3.MOF precusors and structures 3.4.Alternative synthesis strategies 4.Infiltration (post insertion of enzymes in preformed MOFs) 4.1.Early results and the advantages of MOFs for infiltration 4.2.Tuning the framework structure in infiltrated enzyme@MOF biocomposites 4.3.Towards applications of infiltrated enzyme@MOFs 5.Surface bound enzymes 5.1. Immobilization via physical adsorption 5.2. Immobilization via coordinate bonds 5.3. Immobilization via covalent bonding 5.4. Enzymatic activity upon surface-immobilization 6. Multicomponent biocomposites 7. Characterization of MOF immobilized enzymes 7.1. Overview 7.2. Key immobilization parameters 7.3. One-pot enzyme MOF formation 7.4. Highlights of MOF-immobilized enzyme performance 7.4.1. Experimental determination of key immobilization parameters 7.4.1.1. Determination of protein concentrations 7.4.1.2. Activity determination 7.4.2. Advanced characterization of immobilized enzymes 7.4.2.1. Apparent enzyme kinetics 7.4.2.2. Structural analysis and localization o...

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Boosting performance of self-powered biosensing device with high-energy enzyme biofuel cells and cruciform DNA

Cited by 65 publications

References 51 publications

Recent Applications of Carbon Nanomaterials for microRNA Electrochemical Sensing

Recent Applications of Carbon Nanomaterials for microRNA Electrochemical Sensing

Re-engineering Electrochemical Aptamer-Based Biosensors to Tune Their Useful Dynamic Range via Distal-Site Mutation and Allosteric Inhibition

Metal–Organic Framework-Based Enzyme Biocomposites

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