The aim of this study was to provide molecular detection and characterization of the goat isolate of Taenia hydatigena from Ankara province of Turkey. For this purpose, PCR amplification of small subunit ribosomal RNA (rrnS) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed in a one-month-old dead goat. According to rrnS-PCR results, parasites were identified as Taenia spp., and partial sequence of mt-CO1 gene was corresponding to T. hydatigena. At the end of the study, we concluded that molecular tools can be used to define species of parasites in cases where the key morphologic features cannot be detected. Nucleotide sequence data of Turkish goat isolate of T. hydatigena was submitted to GenBank for other researchers interested in this subject. By this study, molecular detection and characterization of T. hydatigena was done for the first time in Turkey.

No abstract

-In this study, we aimed to determine the prevalence of Nosema spp. in honeybees of Turkey. For this aim, adult honeybee (Apis mellifera ) samples were collected from 1621 colonies within 95 apiaries located in 22 provinces of Turkey. Samples were examined microscopically. In case of positivity, spore identification was done by multiplex PCR. At the end of microscopic examination, Nosema spp. spores were detected in 7 out of 22 provinces (31.8 %), and 16 out of 95 colonies (16.8 %) that represent 1621 colonies. According to PCR results, 1 out of 16 isolates (6.25 %) was Nosema apis , and 15 out of 16 isolates (93.75 %) were Nosema ceranae . The result of our study indicated that N.ceranae is the dominant species in Turkey.Nosema apis / Nosema ceranae / Multiplex PCR / Turkey

Toxoplasmosis is one of the most important foodborne parasitic diseases of humans. In particular, sheep muscles are significant sources of infection in the transmission of toxoplasmosis. Carnivorism is the most important transmission route for human populations. The aim of this study was to detect the prevalence of Toxoplasma gondii tissue cysts in sheep meats in retail stores of Turkey. A total of 250 boneless sheep meat samples were purchased from randomly selected retail stores in different locations of Ankara and Kırıkkale provinces of Turkey. The homogenized meat samples were centrifuged with Percoll dilutions. The tissue cysts were removed by pipette and analyzed under light microscope. Additionally, nested PCR was used to detect T. gondii DNA in the meat samples. Tissue cysts were observed in 21.2% of the meat samples with Percoll gradient centrifugation. The prevalences of the tissue cysts were detected as 20.8% in the meat samples obtained from Ankara and 22.4% from Kırıkkale (P > 0.05). T. gondii DNA was detected in 40.8% of the meat samples with nested PCR.

SummaryThis study was planned to identify six parasites detected in horse mackerels (Trachurus trachurus) sold for human consumption in Erzurum province. DNA extraction was performed on six parasites diagnosed as nematode. Species identification was done by PCR and PCR-RFLP analysis of ITS (ITS-1, 5.8S subunit rRNA gene and ITS-2) region of ribosomal DNA (rDNA) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) and mt-CO2 genes. According to the PCR and PCR-RFLP results, all parasites were identified as Anisakis pegreffii. Partial sequence of mt-CO1 gene of one randomly selected parasite was corresponding with A. simplex and mt-CO2 genes of two parasites were corresponding with A. pegreffii. With this study, A. pegreffii was molecularly detected for the first time in Turkey. Keywords: Anisakis pegreffii, Fish, PCR, PCR-RFLP, Sequencing Türkiye'nin Erzurum İlinde İnsan Tüketimi İçin Satılan İstavrit Balıklarında (Trachurus trachurus) Anisakis pegreffii'nin Moleküler Tanısı ÖzetBu çalışma, Erzurum ilinde insan tüketimi için satılan istavrit balıklarında (Trachurus trachurus) tespit edilen altı parazitin tür tanısını yapmak amacıyla planlanmıştır. Nematod olarak belirlenen altı parazitten DNA ekstraksiyonu yapılmıştır. Tür tanısı, rDNA'nın ITS bölgelerinin (ITS1, 5.8 alt ünite rRNA ve ITS2) PCR ve PCR-RFLP analizleri ve mitokondrial sitokrom c oksidaz alt ünite 1 (mt-CO1) ve mt-CO2 genlerinin kısmi olarak sekanslanması ile yapılmıştır. PCR ve PCR-RFLP sonuçlarına göre tüm parazitlerin Anisakis pegreffii olduğu belirlenmiştir. Rastgele seçilen bir parazitin mt-CO1 geninin kısmi sekans sonucu A. simplex, iki parazitin mt-CO2 geninin kısmi sekans sonuçları ise A. pegreffii ile uyuşmuştur. Bu çalışma ile A. pegreffii Türkiye'de ilk kez moleküler olarak tespit edilmiştir.

No abstract

SummaryCystic echinococcosis is one of the most important helminthozoonosis both in Turkey and all around world. Polymerase Chain Reaction (PCR) amplification of mitochondrial 12S rRNA (mt-12S rRNA) and small subunit ribosomal RNA (rrnS) genes were used for molecular characterization of 28 isolates of Echinococcus granulosus obtained from 19 sheep in Kilis province. And also partial mitochondrial cytochrome c oxidase subunit I (mt-CO1) genes of randomly selected two sheep isolates were amplified and sequenced. At the end of the study, all sheep isolates were detected as E. granulosus sensu stricto (G1-G3 complex). Keywords: Echinococcus granulosus, Kilis, Sheep, PCR Kilis İlinde Echinococcus granulosus'un Koyun İzolatlarının Moleküler Karakterizasyonu ÖzetKistik ekinokokkozis Türkiye'de ve dünyada en önemli helmintozoonozlardan biridir. Kilis ilindeki 19 koyundan elde edilen 28 Echinococcus granulosus izolatının moleküler karakterizasyonu için mitokondrial 12S rRNA (mt-12S rRNA) ve küçük altünite ribosomal RNA (rrnS) genleri polimeraz zincir reaksiyonu (PZR) ile çoğaltıldı. Ayrıca rastgele seçilen iki koyun izolatının mitokondrial sitokrom c oksidaz altünite 1 (mt-CO1) geni kısmi olarak çoğaltılıp dizi analizi yapıldı. Çalışma sonucunda tüm koyun izolatları E. granulosus sensu stricto (G1-G3 kompleks) olarak belirlendi.

Hepatic coccidiosis caused by Eimeria stiedae, results in severe infection, outbreaks and deaths in young rabbits. Disease is diagnosed by time consuming methods like fecal examination, oocyst sporulation and necropsy. The aim of this study was to detect E.stiedae with species specific Polymerase Chain Reaction (PCR), without waiting oocyst sporulation and necropsy. Impression smears were prepared from liver nodules and stained with giemsa. Fecal samples were collected from the intestines and examined by centrifugal flotation with salt solution to detect Eimeria spp. oocysts. Then oocysts were sporulated in 2.5% (w/v) aqueous potassium dichromate (K 2 Cr 2 0 7 ). Primers specific to E.stiedae were used in PCR reaction. Eimeria spp. oocysts were detected after the examination of impression smears and bowel content. E.stiedae was diagnosed after the measurement of sporulated oocyst and PCR. At the end of the study, we think that; PCR will be very valuable for the rapid and true diagnosis of hepatic coccidiosis in rabbits.