Mitochondria play a critical role in several cellular processes and cellular homeostasis. Mitochondrion dysfunction has been correlated with numerous metabolic diseases such as obesity and type 2 diabetes. MicroRNAs are non-coding RNAs that have emerged as key regulators of cell metabolism. The microRNAs act as central regulators of metabolic gene networks by leading to the degradation of their target messenger RNA or repression of protein translation. In addition, vesicular and non-vesicular circulating miRNAs exhibit a potential role as mediators of the cross-talk between the skeletal muscle and other tissues/organs. In this review, we will focus on the emerging knowledge of miRNAs controlling mitochondrial function and insulin signaling in skeletal muscle cells. J. Cell. Physiol. 232: 958-966, 2017. © 2016 Wiley Periodicals, Inc.

Mitochondrial number and shape are constantly changing in response to increased energy demands. The ability to synchronize mitochondrial pathways to respond to energy fluctuations within the cell is a central aspect of mammalian homeostasis. This dynamic process depends on the coordinated activation of transcriptional complexes to promote the expression of genes encoding for mitochondrial proteins. Recent evidence has shown that the nuclear corepressor NCoR1 is an essential metabolic switch which acts on oxidative metabolism signaling. Here, we provide an overview of the emerging role of NCoR1 in the transcriptional control of energy metabolism. The identification and characterization of NCoR1 as a central, evolutionary conserved player in mitochondrial function have revealed a novel layer of metabolic control. Defining the precise mechanisms by which NCoR1 acts on energy homeostasis will ultimately contribute towards the development of novel therapies for the treatment of metabolic diseases such as obesity and type 2 diabetes.

Abstract-The aim of the present study was to assess the effects of prior ingestion of coconut water on fluid retention and exercise capacity in the heat as well as signs of gastrointestinal distress. Eight physically active men were recruited (age 23 ± 3 years, height 176 ± 6 cm, body mass 78 ± 7 kg) and performed three exercise capacity trials on a cycle ergometer in the heat (34 ± 1°C) after the ingestion of one of the following drinks: a) plain water (PW), b) flavored drink (FD), and c) coconut water (CW). Ingestion of CW resulted in a longer time to exhaustion (p=0.029). Likewise, participants achieved a higher heart rate in the CW session when compared to the other trials (PW 183 ± 5 bpm, FD 184 ± 8 bpm, and CW 189 ± 8 bpm, p<0.05) and a reduced urine output after the coconut water ingestion (PW 214 ± 85 ml, FD 267 ± 90 ml, and CW 161 ± 73 ml, p<0.05) indicating a higher fluid retention of coconut water in comparison to plain water and the flavored drink. These results demonstrate that previous ingestion of coconut water improves exercise capacity in the heat and provide a reduced urine output in comparison to plain water and flavored drink. Also there is no evidence for GI distress. Keywords: hydration, performance, temperatureResumo-O objetivo do presente estudo foi avaliar os efeitos da ingestão prévia de água de coco sobre a retenção de líquidos e capacidade de resistência ao exercício no calor, bem como sinais de desconforto gastrointestinal. Oito homens fisicamente ativos foram recrutados (idade 23 ± 3 anos, altura 176 ± 6 cm, massa corporal 78 ± 7 kg) e realizaram três sessões de capacidade de exercício em uma bicicleta ergométrica no calor (34 ± 1° C) após a ingestão de uma das seguintes bebidas: a) água pura (PW), b) bebida com sabor (FD), e c) água de coco (CW). A ingestão de CW resultou num maior tempo até a exaustão (p = 0,029). Da mesma forma, os participantes alcançaram uma frequência cardíaca mais alta na sessão de CW quando comparado com as outras sessões (PW 183 ± 5 bpm, FD 184 ± 8 bpm, e CW 189 ± 8 bpm, p <0,05) e uma taxa de produção de urina reduzida após a ingestão de água de coco (PW 214 ± 85 ml, FD 267 ± 90 ml, e CW 161 ± 73 ml, p <0,05), indicando uma maior retenção de líquidos na sessão água de coco em comparação com água pura e bebida com sabor. Estes resultados demonstram que a ingestão prévia de água de coco melhora a capacidade de exercício no calor e proporciona uma menor produção de urina em comparação com a água pura e bebida com sabor. Também não houve nenhuma evidência de desconforto gastrointestinal. Palavras-chave: hidratação, desempenho, temperaturaResumen-El objetivo del presente estudio fue evaluar los efectos de la ingesta previa de agua de coco en la retención de líquidos y la capacidad de ejercicio al calor, así como señales de malestar gastrointestinal. Ocho hombres físicamente activos fueron reclutados (edad 23 ± 3 años, altura 176 ± 6 cm, la masa corporal de 78 ± 7 kg) y se realizaron tres ensayos de capacidad de ejercicio en un cicloergómetro con el calor (34 ± ...

The method with the 324-gram mass dropped from a 70-cm height onto the gastrocnemius muscle of rats seems to be the most suitable for the production of muscle injury in these animals after 72 hours, showing an important inflammatory infiltrate.

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