Environmental pollution results in serious health hazards to animals and blood analysis serves as a good alternative for health status assessment. The target of this study was to analyze the concentration of selected metals in equine blood, to analyze the blood parameters and to find possible correlations. Blood samples were collected from the vena jugularis of healthy adult horses. The highest concentration of all elements was found in whole blood (Cu 3.84 ± 0.90 mg L(-1); Cd = 0.81 ± 0.90 mg L(-1); Zn 26.67 ± 14.12 mg L(-1); Pb 9.33 ± 5.76 mg L(-1)). Higher concentrations of copper, cadmium, zinc and lead were detected in blood clots compared to blood sera (44.04%). A similar tendency was found for cadmium (50%), zinc (13.08%) and lead (46.02%), which showed generally higher concentrations in blood clots (cells). Correlation analysis proved some relations between analyzed elements. In blood clots there is a strong positive correlation between Cd - Pb (r = 0.93) and Zn - Pb (r = 0.71) was detected. For biochemical and hematological parameters mainly medium correlations were detected. Obtained results prove different correlations of analyzed elements in blood components as well as the effect on parameters of blood biochemical and hematological profiles.
Amygdalin has been one of the most popular “alternative cancer cures” in many European and South American countries. Its anticancer, anti-inflammatory activity and other medicinal benefits have been known for many years. The objective of this in vitro study was to examine the potential impact of amygdalin on the cell viability and production of steroid hormone testosterone by porcine ovarian granulosa cells. Granulosa cells were isolated from porcine ovaries and subsequently cultured without (control) or with amygdalin at various doses (1; 10; 100; 1000 and 10 000 μg/mL) for 24 h. The cell viability was determined by alamarBlueTM reagent and release of testosterone was assayed by ELISA. Obtained results showed a significant (P
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