Stimulatory Effect of Amygdalin on the Viability and Steroid Hormone Secretion by Porcine Ovarian Granulosa Cells in Vitro

Halenár, Marek; Tušimová, Eva; Nynca, Anna; Sadowska, Agnieszka; Ciereszko, Renata E.; Kolesárová, Adriana

doi:10.15414/jmbfs.2016.5.special1.44-46

Cited by 9 publications

(9 citation statements)

References 12 publications

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“…In past decades, amygdalin has been examined extensively. It has possible modulatory impact on cell viability [25] and the steroid production in porcine ovaries in vitro, as Halenár et al [26,27] reported in their study. Michalcová et al [28] published, that natural substances present in apricot seeds may be involved in ovarian folliculogenesis mechanisms of rabbits through inhibition of FSH (follicle-stimulating hormone) levels.…”

Section: Resultsmentioning

confidence: 74%

Human Urine Alterations Caused by Apricot Seeds Consumption

Tušimová

Zbyňovská

Kováčik

et al. 2017

Advanced Research in Life Sciences

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Amygdalin is a cyanogenic glucoside derived from various plants seeds. It is a natural product that owns antitumor activity, it has also been used for the treatment of asthma, bronchitis, emphysema, leprosy and diabetes and produces antitussive and antiasthmatic effects. The present in vivo study was designed to reveal whether amygdalin in apricot seeds has got an effect on human urine composition, pH value and urine associated health status after twelve weeks of oral administration. The study group finally consisted of 12 healthy adult volunteers. All participants consumed 60 mg/kg of body weight of bitter apricot seeds daily (approximately 3.0 mg/kg of amygdalin) during 12 weeks. Two urine collections were carried out (at the beginning of the experiment and after 84 days). Quantification of urine calcium, magnesium, phosphorus, sodium, potassium, chlorides, urea and pH value after apricot seeds supplementation was performed. Paired t-test showed, that consumption of bitter apricot seeds during 84 days had a significant (P < 0.01) effect on amount of calcium excreted in urine, though this decrease shifted its level from elevated mean value in control collection into normal physiological range. No significant changes were observed in other parameters after apricot seed ingestion.

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Section: Resultsmentioning

confidence: 74%

Human Urine Alterations Caused by Apricot Seeds Consumption

Tušimová

Zbyňovská

Kováčik

et al. 2017

Advanced Research in Life Sciences

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“…There is a significant amount of evidence for the role of amygdalin on male reproductive functions in animals [21,22,38,43,60], as well as female reproductive system in vivo and in vitro [23,53,[61][62][63][64]. Amygdalin application positively affects ovarian cell viability and stimulates testosterone release by porcine ovarian granulosa cells; therefore, amygdalin can exert a potential effect on cellular growth and the process of ovarian steroidogenesis in vitro [62]. In addition, amygdalin has an evident effect in the treatment of endometriosis, an aggressive disorder associated with infertility, pelvic pain, and intra-abdominal adhesions in women of reproductive age [53].…”

Section: Effects On the Female Reproductionmentioning

confidence: 99%

“…28 days male rabbits antioxidant effect positive [43] 5 mg/kg/week i.p. 42 days female rats protective effect against endometriosis positive [53] In vitro studies 0.5, 1, 2.5 and 5 mg/mL 5 h rabbit spermatozoa stimulatory effect positive [60] 1, 10, 100, 1000 and 10,000 µg/mL 24 h porcine ovarian cells stimulatory effect positive [61][62][63][64]…”

Section: Effects On the Female Reproductionmentioning

confidence: 99%

“…Amygdalin significantly increases polyhydroxyalkanoates which stimulates peripheral blood T-lymphocytes to secrete IL-2 and interferon γ (IFN-γ), and then reduce the secretion of TGF-β1 [16]. In addition, this phytocompound is involved in the regulation of female reproductive functions through endocrine regulators such as TGF-β signaling pathway [16] and ovarian steroid hormones [61][62][63][64]. The studies have revealed the potential impact of amygdalin on cellular growth as well as its mechanism of action in the process of ovarian steroidogenesis.…”

Section: Application In Reproductive Biology and Medicinementioning

confidence: 99%

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The Multiple Actions of Amygdalin on Cellular Processes with an Emphasis on Female Reproduction

2021

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The present review summarizes the current knowledge on the provenance and properties, metabolism and toxicity, mechanism of action, physiological, and therapeutic roles of amygdalin—a molecule present in the seeds of apricot and other plants—with an emphasis on the action of amygdalin on reproductive processes, particularly in the female. Amygdalin influences physiological processes including female reproduction at various regulatory levels via extra- and intracellular signaling pathways regulating secretory activity, cell viability, steroidogenesis, proliferation, and apoptosis. On the other hand, while being metabolized in the body, amygdalin releases significant amounts of cyanide, which may lead to acute health hazard in those individuals who may be at risk. Despite some contradictions in the available data about benefits and toxic effects of amygdalin, its potential applicability at low doses may present a promising tool for regulation of various reproductive and other physiological processes including disease management primarily in cancer phytotherapy, animal production, medicine, and biotechnology. However, further research involving carefully designed dose–response studies is required to overcome the possible side effects of amygdalin and assure its safety as a therapeutic agent.

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“…Nowadays, natural biomolecules and their impact on reproductive functions have attracted a huge scientific attention (Kolesarova et al, 2011;Halenar et al, 2016;Packova et al, 2016;Roychoudhury et al, 2018). Naturally-occurring phytocompounds from dietary fruits and vegetables have received public interest for the prevention and treatment of various diseases (Modaeinama et al, 2015;Sharma et al, 2017).…”

Section: Figurementioning

confidence: 99%

Polyphenol-Rich Pomegranate Extract as a Potential Modulator of Steroidogenesis in Human Ovarian Cells

Baldovská¹,

Michalcová²,

Halenár³

et al. 2019

J microb biotech food sci

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Pomegranate represents a rich source of phytocompounds with high medicinal value. Nowadays, many studies have shown that the pomegranate extract also possesses anti-oxidant, anti-inflammatory and anti-proliferative effects on cancer cells, thus leading to increased popularity as a functional food and nutraceuticals. The aim of the study was to determine the biological effect of dry pomegranate extract (at concentrations 5, 10, 20 and 40 µg/ml; for 24h) on the viability of ovarian cells and the secretion of steroid hormones. Cultures of human ovarian granulosa cells (HGL5) and human ovarian carcinoma cells (OVCAR-3) were used such as a model cell system. The metabolic activity was evaluated by AlamarBlueTM assay, the release of steroid hormones was assayed by the ELISA method. Experimental results indicated a significant (P≤0.001) increase of proliferation in HGL5 cells after the addition of the extract at the concentrations 5, 10 and 20 µg/ml. Moreover, the number of viable OVCAR-3 cells significantly (P≤0.05; P≤0.01; P≤0.001) decreased after the addition of the extract at the concentrations 10, 20 and 40 µg/ml compared to the control. In addition, the secretion of 17ß-estradiol by the HGL5 cells was significantly (P≤0.05; P≤0.001) increased at all used concentrations of the extract. Despite to increasing of the 17ß-estradiol secretion, progesterone levels produced by the HGL5 cells were not significantly (P≥0.05) affected at all used concentrations of the extract. The current study provided experimental evidence that the pomegranate extract might be a promising candidate as a potential modulator of steroidogenesis and as a potential chemoprotective agent.

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Stimulatory Effect of Amygdalin on the Viability and Steroid Hormone Secretion by Porcine Ovarian Granulosa Cells in Vitro

Cited by 9 publications

References 12 publications

Human Urine Alterations Caused by Apricot Seeds Consumption

Human Urine Alterations Caused by Apricot Seeds Consumption

The Multiple Actions of Amygdalin on Cellular Processes with an Emphasis on Female Reproduction

Polyphenol-Rich Pomegranate Extract as a Potential Modulator of Steroidogenesis in Human Ovarian Cells

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