Populasi ternak kambing di Kecamatan Batu Keliang Utara Lombok Tengah tercatat hanya sekitar 873 ekor. Masyarakat Batu Kliang Utara menginginkan pengembangan ternak yang berbasis teknologi inseminasi buatan (IB), mengingat permintaan pasar akan ternak dan daging kambing yang semakin meningkat. Aplikasi teknologi pada ternak kambing belum berkembang karena sebagian besar mayarakat belum memahami cara penerapannya. Oleh karena itu, telah dilaksanakan pengabdian kepada masyarakat tentang “introduksi teknologi IB pada kambing di Batu Keliang Utara, Lombok Tengah”. Tujuannya adalah memberikan pengetahuan dan keterampilan kepada masyarakat di Kecamatan Batu Keliang Utara tentang prospek, manfaat dan kelebihan teknologi inseminasi buatan (IB) pada ternak kambing. Metode pendekatan yang digunakan adalah penyuluhan dan pendidikan singkat dengan cara ceramah, diskusi dan memberikan contoh tentang hal-hal yang terkait dengan program IB dan upaya untuk meningkatkan efisiensi reproduksi dan produksi serta efisiensi usaha beternak kambing. Materi penyuluhan meliputi pemilihan pejantan dan betina, menadah sperma, menyiapkan betina, deteksi birahi dan peaksanaan IB, serta deteksi kebuntingan. Berdasarkan hasil evaluasi, kegiatan pengabdian kepada masyarakat dinilai cukup berhasil. Evaluasi yang dimaksud antara lain respon dan antusiasme peserta terlihat cukup besar. Para peserta tampak cukup memahami materi yang disuluhkan dan ingin menerapkan teknologi IB dalam kegiatan beternak yang bernilai ekonomis. Besarnya sambutan dan respon para peserta penyuluhan merupakan foktor pendukung yang cukup besar untuk diterima dan diterapkannya teknologi IB dalam manajemen reproduksi ternak mereka ke depan. Kesimpilan dari kegiatan pengabdian kepada masyarakat ini adalah diduga terjadi menambah wawasan dan pemahaman masyarakat tentang pentingnya penerapan teknologi inseminasi buatan dalam meningkatkan reproduktivits dan produktivitas ternak kambing.
The objective of this study was to determines the effect of guava fruit filtrate (GF) supplementation in Tris and Citrate-based extenders on spermatozoa quality of Brangus bull after sex-break. Semen samples (twelve ejaculates) were diluted in Tris-egg yolk (TEY) without GF (control group, T1), TEY with 10% GF (T2), Citrate-egg yolk (CEY) with 10% GF (T3), Citrate with 10% egg yolk and 10% GF (T4), then chill-storage. The variables quality evaluated include spermatozoa progressive motility, visibility, and abnormality. Spermatozoa quality was observed every 24 hours until the spermatozoa motility reached at least 40%. The results showed that progressive motility and viability on the fourth day storage in extender Citrate-egg yolk (CEY) with 10% egg yolk and 10% GF were 50.4 ± 1.6% and 57.1 ± 1.6%, significantly higher (P<0.05) than control, TEY with 10% GF and Citrate with 10% egg yolk. The spermatozoa abnormality was 12.9 ± 0.3%, the lowest in extender Citrate with 10% egg yolk and 10% GF on the fourth-day storage. In conclusion, supplementation of GF to extender improves the spermatozoa quality of Brangus bull after sex activity break for a long time. Moreover, the best quality of spermatozoa is found in supplementing 10% GF to Citrate with 10% egg yolk extender.
The aims of the research were to identify components, factors and relative risk (Odd ratio) which may influence the artificial insemination results. The study were performed in two villages those were Kuranji and Tanah Beak, by identification of components and factors which may contribute to successful artificial insemination results, then the flow chart of successful and obstacle were designed. Based on the flow chart survey was performed to the farmers and inseminators. Parameters evaluated were factors may influence of the result of insemination such as; management, oestrus detection by farmers and insemination by inseminators. The data was tabulated and analised using epidemiology analysis of odd ratios (OR). The results showed that insemination results of Kuranji village do not have the risk to be not pregnant as the odd ratio was less than 1 (OR <1,0), with the range of 0,10 to 0,20. While artificial insemination in Tanah Beak village showed risk of cows to be infertile or non pregnant relatively higher with OR higher than 1 (OR> 1.0), with the range of 1,5 to 2,0. The results showed that the risk to be unsuccessful to produce pregnancy were caused by oestrus detection, feeding management, skill inseminator and experience of farmers. Finally it can be concluded that oestrus detection by farmers, feeding management, insemination by inseminator and experience of farmers may contribut to the high risk fail to produce pregnancy using artificial insemination in Tanah beak village.
Peningkatan mutu reproduksi pada kegiatan perternakan sapi perlu terus menerus digalakkan. Hal tersebut agar terciptanya efisiensi pada usaha produksi ternak sehingga menghasilkan keuntungan finansial yang lebih baik. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan mutu genetik pada ternak sapi melalui inseminasi buatan menggunakan tekhnologi sperma sexing. Kegiatan ini dilakukan melalui penyuluhan, ceramah dan diskusi. Peserta yang menjadi target pelaksanaan kegiatan adalah peternak sapi yang berkecimpung pada bidang tekhnologi reproduksi. Hasil evaluasi menunjukkan bahwa kegiatan pengabdian pada masyarakat ini berjalan cukup baik. Respon dan antusiasme peserta penyuluhan dalam diskusi sangat baik. Penilaian sangat baik karena sambutan dari tokoh atau pemuka masyarakat untuk dapat merealisasikan pengetahuan tentang IB menggunakan sperma sexing pada ternaknya. Kesimpulan dari kegiatan ini adalah terjadinya peningkatan pengetahuan dan pemahaman peserta tentang teknologi inseminasi buatan menggunakan metode sperma sexing pada ternak sapi. Penilaian ini berdasarkan respon peserta dan pemuka masyarakat, sehingga hal ini merupakan faktor pendukung keberhasilan pelaksanaan IB pada waktu yang akan datang.
The aim of this study was to determine the differences ofestrous response, service per conception (S/C), non return rate (NRR),and conceptionrate (CR) between Bali cattle heifersandcowssynchronized using intra-vaginal progesterone hormone. Two groups of Bali cattle, 15 heifers and 15 cows, were used to examine the response differences of estrous synchronization using progesterone hormone on reproductive performance, between different parity. Each group was given progesterone dose of 1 mg/kg body weight. The data observed were estrousbehavior (estrousintensity, onset of estrous, estrous percentage), and value of S/C, NRR, and CR were analyzed by statistical analysis (t-test). The result shows the estrous intensity was significantly different (P<0.05) between the heifers and cows. The average estrousintensity score of the heifer was 2.87±0.35, while the cows was 2.27±0.45. There was significantly different (P <0.05) in onset of estrous in the cows 41.5±5.18 hours, while in the heifers was 50.67±3.5 hours. The estrouspercentage of the heifers and cows was the same, both of them was 100%. There was no significant difference (P>0.05) in S/Cvalue showed by the heifers (1,31±0,45)and the cows (1,50±0,50). The average value of NRR, for heifers and cowswas 86.67% and 80.00%, respectively. The average value of CRfor heifers and cowswas 86.67% and 80.00%, respectively.
Penerapan teknologi inseminasi buatan (IB) menggunakan semen sapi eksotik sering menyebabkan gagal bunting, abortus, disokia dan gangguan kelahiran lainnya. Akibatnya adalah sering terjadi kawin berulang, kerugian dari sisi tenaga, biaya dan waktu, tertundanya regenerasi selama umur produktif, serta inefisiensi reproduksi. Masalahnya, peternak tidak banyak mengetahui faktor-faktor penyebab kegagalan hasil IB, sehingga perlu diberikan penyuluhan. Memberikan informasi, pemahaman dan keterampilan kepada masyarakat tentang cara-cara memanage reproduksi yang baik dan benar untuk mencegah terjadinya kawin berulang dan distokia pada sapi pasca inseminasi buatan. Kegiatan dilaksanakan dengan metode partisipatif melalui penyuluhan, diskusi dan pembinaan singkat, bertempat di Desa Sintung yang merupakan pusat kegiatan dan sekretariat kelompok tani-ternak di Kesamatan Pringgarata. Khalayak sasaran adalah ketua dan anggota kelompok tani-ternak dan masyarakat petani lainnya. Pengabdian kepada masyarakat ini cukup berhasil. Para peserta sangat tertarik pada materi yang disuluhkan. Setelah penyuluhan peserta memperoleh informasi, pemahaman dan keterampilan untuk meningkatkan keberhasilan IB. Peserta disuluhkan tentang cara pemilihan calon induk resepien, memilih jenis semen pejantan, manajemen induk bunting, kandang bunting dan melahirkan. Perlu catatan perkawinan, mengenal tanda-tanda dan prediksi kelahiran, penanganan kelahiran oleh dokter hewan atau paramedis hewan. Faktor pendukung kegiatan adalah antusiasme peserta penyuluhan dan diskusi, sedangkan faktor penghambatnya adalah kejadian wabah PMK yang masif hampir di seluruh wilayah kecamatan yang sedikit membatasi keleluasaan kegiatan. Kegiatan pengabdian berjalan baik dan lancar. Para peserta memperoleh manfaat ilmu pengetahuan tentang manajemen reproduksi untuk mencegah terjadinya kawin berulang.
The purpose of this research was to determine the effect of Pentoxyfilline in maintaining the quality (motility, viability, morphology and plasma membrane intact) of Bali bull spermatozoa separated with albumine. A Completely Random Block Design was used in this study with two main factors (upper layer and bottom layer), diluents with four doses of pentoxyfilline (0, 15, 20 and 25 μM) with six replications. Evaluation of spermatozoa was performed by characterizing motility, viability, morphology, plasma membrane intact. Result of the study showed that the effect of four doses pentoxyfilline on the quality of Bali bull spermatozoa was non significant (p>0,05). However, the addition of 25μM pentoxyfilline was found to give the best effect compared to 15 μM, 20 μM and control. Result of the study also indicated that spermatozoa separated in the upper layer was signifiqantly higher (p<0,01) than that of separated in the bottom layer with the average motility,
This study aimed to analyze and sequence a protein with molecular weight of approximately 30 kDa found in the saliva of the Indonesian local goat (Capra hircus) using matrix-assisted laser desorption/ionization-time of flight/time of flight-mass spectrometry (MALDI-TOF/TOF-MS) and related techniques. Saliva was collected from 50 healthy male and female local goats of 4-30 months old. The results showed that the average value of the total saliva protein was 669 ± 251.293 µg/mL, in the range of 350-1550 µg/mL. Mascot search and NCBI BLAST search revealed that the peptides were "PREDICTED: short palate, lung, and nasal epithelium carcinomaassociated protein 2A-like", with Mr = 26.656 kDa and pI = 4.57, which was 100% identical to Ovis aries. In addition to this, the peptide was also closely related to "TPA: common salivary protein BSP30 form d". Overall, the peptides belong to the BPI-protein superfamily, whose major roles are in innate immunity. Whether or not the identical state of the peptide in this study with those found in several other ruminants describes an evolutionary process that is common in ruminants still needs to be studied further.
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