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Abstract

The purpose of this research was to determine the effect of Pentoxyfilline in maintaining the quality (motility, viability, morphology and plasma membrane intact) of Bali bull spermatozoa separated with albumine. A Completely Random Block Design was used in this study with two main factors (upper layer and bottom layer), diluents with four doses of pentoxyfilline (0, 15, 20 and 25 μM) with six replications. Evaluation of spermatozoa was performed by characterizing motility, viability, morphology, plasma membrane intact. Result of the study showed that the effect of four doses pentoxyfilline on the quality of Bali bull spermatozoa was non significant (p>0,05). However, the addition of 25μM pentoxyfilline was found to give the best effect compared to 15 μM, 20 μM and control. Result of the study also indicated that spermatozoa separated in the upper layer was signifiqantly higher (p<0,01) than that of separated in the bottom layer with the average motility,

Molecular Verification of Spermatozoa Sexing Method in Pesisir Cattle Bull Using Bovine Serum Albumin Column

“…Preserving the purity of Pesisir cattle and enhancing their productivity is crucial for meeting the increasing beef demand in Indonesia. These can be achieved through the application of reproductive biotechnology, known as spermatozoa sexing (Rodiah et al 2015). Spermatozoa sexing represents a promising reproductive technology that enables the production of livestock with desired sexes, catering to the specific needs of breeders, such as obtaining bulls for beef or female cattle for dairy purposes.…”