Cancer is a disease that is characterized by the existence of damage and cell abnormality in growth and differentiation. Liver cancer is a disorder of hepar tissue derivated from its tumors. Taurine is known as antioxidant but its role as anticancer needs to be explored more as well the role of Annona muricata leaf extract which is believed to have its role as anticancer substance. This research, therefore, aimed to explore the effect of taurine and Annona muricata leaf extract on the hepar histopathology of male mice (Mus musculus) induced by benzo(α)pyren in vivo. This research was carried out by using a complete randomized design, which consisted of 5 treatment groups which was repeated 5 times. Group I was given 0.2 mL corn oil for 15 days, group II was induced by benzo(α)pyren without taurin nor A. Muricata leaf extract for 10 days, group III was given 7.8 mg taurine/BW/day (twice a day) starting from the 15 th days before the induction of benzo(α)pyren, group IV, after induced with benzo(α)pyren, taurine was given with dosage of 7.8 mg/BW/day, group V, after induced with benzo(α)pyren, soursop leaf extract was given with amount of 277.8 mg/BW/day. Data analyzed by Kruskal-Wallis test and one way ANOVA with Fisher test (p>0.05). The results indicated that taurine had ability to recover the liver tissue induced by benzo(α)pyren as (carcinogenic) while, Annona muricata leaf extract had not shown any recover of tissue damage. ABSTRAKKanker merupakan suatu penyakit yang ditandai dengan adanya kerusakan dan ketidaknormalan sel dalam mengatur pertumbuhan dan diferensiasinya. Kanker hati adalah gangguan pada hepar yang berawal dari tumor hepar. Taurin diketahui sebagai senyawa antioksidan, namun perannya dalam antikanker perlu dieksplorasi lebih jauh, demikian pula dengan ekstrak daun sirsak yang telah diminati oleh masyarakat sebagai bahan antikanker. Penelitian ini bertujuan untuk mengetahui peran senyawa taurin dan ekstrak daun sirsak terhadap gambaran histopatologi hepar mencit (Mus musculus) yang diinduksi benzo(α)piren secara in vivo. Penelitian ini dilaksanakan dengan menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 5 kelompok perlakuan dengan masing-masing memiliki 5 ulangan, Kelompok I diberi 0,2 mL minyak jagung selama 15 hari, kelompok II diinduksi dengan benzo(α)piren tanpa pemberian bahan uji selama 10 hari, kelompok III pemberian taurin 7,8 mg/BB/hari (2 kali/hari) sejak 15 hari sebelum induksi benzo(α)piren), kelompok IV diinduksi benzo(α)piren dan dilanjutkan dengan pemberian taurin dosis 7,8 mg/BB/hari (2 kali/hari), kelompok V diinduksi benzo(α)piren, dilanjutkan pemberian daun sirsak dosis 277,8 mg/BB/hari. Data dianalisis dengan metode statistik menggunakan uji ANOVA satu arah
Penelitian ini bertujuan untuk mengetahui laju pertumbuhan dan kandungan lipid pada ketiga jenis mikroalga yang dikultur pada media dengan salinitas yang berbeda. Penelitian dilakukan dengan menggunakan metode rancangan acak lengkap faktorial dengan 3 perlakuan yaitu salinitas 20, 30, dan 40 ppt pada mikroalga jenis Nannochloropsis sp., Tetraselmis sp., dan Porpyridium sp masing-masing sebanyak 3 kali ulangan. Mikroalga dikultur pada skala laboratorium selama 8 hari setelah itu dipanen untuk diukur kadar lipidnya. Hasil kultur selama 8 hari menunjukkan laju pertumbuhan spesifik rata-rata tertinggi pada Nannochloropsis sp. terdapat pada salinitas 40 ppt yaitu 12%/hari, sedangkan untuk Tetraselmis sp. tertinggi pada salinitas 20 ppt yaitu 7%, dan untuk Porpyridium sp. tertinggi pada salinitas 30 ppt yaitu 5%/hari. Berdasarkan hasil penelitian diperoleh jumlah lipid tertinggi terdapat pada Tetraselmis sp. pada salinitas 20 ppt yaitu sebesar 2,64% dan jumlah lipid terendah terdapat pada Tetraselmis sp. pada salinitas 40 ppt yaitu sebesar 0,19%. Dari hasil penelitian dapat disimpulkan bahwa laju pertumbuhan spesifik rata-rata per hari tertinggi terdapat pada jenis Nannochloropsis sp pada salinitas 40 ppt yaitu sebesar 12% dan jumlah lipid tertinggi terdapat pada mikroalga jenis Tetraselmis sp. pada salinitas 20 ppt yaitu sebesar 2,64%.
<p>Taurine in diet affected increasing in body weight and length as well as survival rate of juveniles or adults of many marine fishes. It is explained that taurine presumably increased in their ability to tolerate the hypertonicity of seawater as well as to improve their metabolism which leaded to induce maturation. In order to elucidate the effect of taurine physiological universally in the fishes, early study was conducted on freshwater fishes namely gourami (Osprhonemus goramy) and tilapia (Oreochromis niloticus). The growth was determined by their body weight and morphological changes included the length and width of their body. The reproductive status was determined by measuring fish gonadosomatic indexes (GSI) (only for tilapias). Eight experimental groups were chosen consisted of two groups with commercial fish food with and without taurine and two groups with natural fish food (consisted of 50% Xanthosoma sagittifolium) with and without taurine. Amount of taurine given was 0.4 mg/fish/day. Complete randomized design with factorial 2x2 was applied to this study with 40 gourami juveniles (@ 7 grams in weight) and 40 tilapias (@ 50 grams in weight) were used for the experimental units. The study was conducted in 2 months. Analysis variances and LSD and/or t-test at 5% were used to determine the differences among experimental groups. The results indicated that taurine groups increased their body weight for 25-66% compared to the control (no taurine) as well as their body length and width (p< 0.05). Yet, the GSI of tilapias was not shown any significantly different. In this early study we indicated that taurine affected the growth in juvenile gourami and tilapia. However, further experiments related with physiological changes and reproductive status was still needed to complete the study. </p><p><strong>Keywords</strong>: Taurine, gourami, tilapia, growth rate</p>
Benzo(α)pyrene is one of carcinogenic PAH substances which commonly produced by many different sources of polutant. The study was conducted in order to determine the effect of methanol extraction of Clerodendrum sp and Acanthus illicifolius which known to be two of common mangrove plants on the blood profile of male mice induced by benzo(α)pyrene (one of polycyclic aromatic hydrocarbon/PAH). Thirty male mice (Mus musculus) of ddy strain from Lampung Vetenary Agency were used to conduct the study. Completely randomized design was assigned for this study. Mice were grouped into 5 different group treated with PAH injection, one groups used as control without any injection, one as postitif group with PAH injection but not given with any methanol extraction and the last three group were given with methanol extraction of Clerodendrum and Acanthus and taurine prior PAH injection. All the animal study were kept in room temperature with 12:12 light cycle and fed and water ad libitum. Observation of their blood cells and protein profile was made after 14 days. Data was analyzed by One-Way Anova at 5% followed by Fisher’s at level of p < 0.05. The study showed that methanol extraction of Clerodendrum, Acanthus and taurine were able to return back either the number of redblood or whiteblood cells. However, the protein profile of the blood was not able to show any differences among treatment groups from which 14 different bands were observed under SDS-PAGE method.
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