Antioxidant Effect of Clerodendrum sp and Acanthus illicifolius Methanol Extraction on Blood Profile of Male Mice Induced by Benzo(α)pyrene

Abstract

Benzo(α)pyrene is one of carcinogenic PAH substances which commonly produced by many different sources of polutant. The study was conducted in order to determine the effect of methanol extraction of Clerodendrum sp and Acanthus illicifolius which known to be two of common mangrove plants on the blood profile of male mice induced by benzo(α)pyrene (one of polycyclic aromatic hydrocarbon/PAH). Thirty male mice (Mus musculus) of ddy strain from Lampung Vetenary Agency were used to conduct the study. Completely randomized design was assigned for this study. Mice were grouped into 5 different group treated with PAH injection, one groups used as control without any injection, one as postitif group with PAH injection but not given with any methanol extraction and the last three group were given with methanol extraction of Clerodendrum and Acanthus and taurine prior PAH injection. All the animal study were kept in room temperature with 12:12 light cycle and fed and water ad libitum. Observation of their blood cells and protein profile was made after 14 days. Data was analyzed by One-Way Anova at 5% followed by Fisher’s at level of p < 0.05. The study showed that methanol extraction of Clerodendrum, Acanthus and taurine were able to return back either the number of redblood or whiteblood cells. However, the protein profile of the blood was not able to show any differences among treatment groups from which 14 different bands were observed under SDS-PAGE method.

Splenic and Leucocytic Responses in Wistar Rats Exposed to Chronic Doses of Hydromethanol Extract of Helianthus annuus Leaf in Feed

“…The leucocyte count is higher in the day time than the night hours, while stress produce increase in lymphocyte concentration and decrease in neutrophil concentration [22]. Poisonous chemical like benzo(α)pyrene and dichlorvus elicit increase in leucocyte concentration [24,25]. In rats, the lymphocyte and neutrophil represent 60-75% and 12-38% of leucocyte count respectively, while eosinophil, basophil and monocyte levels vary within 0-7% of leucocyte count [22,26].…”

PROBIOTIC CANDIDATE PROTEOLYTIC Bacillus sp. COLLECTED FROM MANGROVE OF MARGASARI, LAMPUNG

“…These microbes possibly believed to have beneficial uses such as probiotic, antibiotic, and bioactive products and else (Subagiyo, Muhammad, & Wilis, 2017). The recent study also indicated that some mangrove shrubs, such as Acanthus ilicifolius contain antioxidant (Widiastuti, Arifianti, Khairani, Christianto, Ara, & Maharani, 2019). Other studies showed that Bacillus sp.…”

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“…Antioksidan merupakan senyawa yang dapat menangkal efek berbahaya dari radikal bebas. Dalam proses menangkal radikal bebas antioksidan dapat memperlambat atau mencegah proses oksidasi dimana proses oksidasi ini dapat menyebabkan beberapa penyakit dan kerusakan seperti kerusakan protein dan DNA (Widiastuti et al, 2019). Tubuh memiliki antioksidan endogen yang berasal dari dalam tubuh dan dalam menetralkan radikal bebas dapat dibantu oleh antioksidan eksogen (Khaira, 2010).…”