Ebola virus-like particles (VLPs) were produced in insect cells using a recombinant baculovirus expression system and their efficacy for protection against Ebola virus infection was investigated. Two immunizations with 50 ug Ebola VLPs (high dose) induced a high level of antibodies against Ebola GP that exhibited strong neutralizing activity against GP-mediated virus infection and conferred complete protection of vaccinated mice against lethal challenge by a high dose of mouse-adapted Ebola virus. In contrast, two immunizations with 10 ug Ebola VLPs (low dose) induced 5-fold lower levels of antibodies against GP and these mice were not protected against lethal Ebola virus challenge, similar to control mice that were immunized with 50 ug SIV Gag VLPs. However, the antibody response against GP were boosted significantly after a third immunization with 10 ug Ebola VLPs to similar levels as those induced by two immunizations with 50 ug Ebola VLPs, and vaccinated mice were also effectively protected against lethal Ebola virus challenge. Furthermore, serum viremia levels in protected mice were either below the level of detection or significantly lower compared to the viremia levels in control mice. These results show that effective protection can be achieved by immunization with Ebola VLPs produced in insect cells, which give high production yields, and lend further support to their development as an effective vaccine strategy against Ebola virus.

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The aim of this study was to determine the effect of prepartum somatotropin injection in late-pregnant Holstein heifers on metabolism, milk production and resumption of postpartum ovulation. For this study, 31 late-pregnant Holstein heifers were used. The heifers were assigned randomly into two treatments: (1) 500 mg sc injections of somatotropin (somatotropin treatment, n 5 15) at 235 and 221 days, and, if pertinent, at 27 days from expected calving date and (2) no treatment (control group, n 5 16). Blood samples were collected weekly from 25 to 7 weeks after calving. Heifers with progesterone concentrations in plasma above 1 ng/ml in two consecutive postpartum samples were considered as having resumed ovarian activity. A higher proportion (P 5 0.04) of heifers treated with somatotropin resumed ovarian activity in the first 7 weeks post partum (73.3%; 11/15) compared with the control group (37.5%; 6/16). A higher number (P 5 0.02) of heifers in the somatotropin treatment group also ovulated during the first postpartum follicular wave (53.3%; 8/15) compared with the control group (12.5%; 2/16), as indicated by the number of heifers ovulating in the first 3 weeks post partum. Pregnancy rate was not affected by treatments (P . 0.10) and averaged 40.0% (6/15) in somatotropin-treated and 25.0% (4/16) in control heifers when evaluated up to 150 days in milk. Somatotropin treatment increased the average daily milk production by 2.8 kg/cow per day (P , 0.0001) and reduced the somatic cell count (P 5 0.009). Plasma IGF-I was higher (P , 0.05) for somatotropin-treated heifers in the prepartum period. Insulin and body condition score were higher (P , 0.05) and non-esterified fatty acids were lower (P , 0.05) for somatotropin-treated cows in the early postpartum period. In conclusion, somatotropin injection during the prepartum period in late-pregnant Holstein heifers was able to increase the proportion of heifers resuming ovarian activity early post partum, inspite of higher milk production.Keywords: bST, dairy heifers, IGF-I, ovarian activity, primiparous ImplicationsPhysiological changes during the transition period can have a great impact on health and performance. In our study, prepartum injection of somatotropin reduced negative energy balance (NEB) intensity as indicated by several metabolic markers evaluated in the postpartum period. A more intense NEB is usually associated with a delay in resumption of normal ovarian function. In this way, we observed a higher proportion of somatotropin-treated heifers ovulating earlier.Moreover, cows treated with somatotropin prepartum increased milk production. Along with earlier resumption of estrous cycles, this strategy can have an important impact on farm profitability. IntroductionDuring the early postpartum period, high-producing cows experience a period of negative energy balance (NEB) that occurs as the requirements for milk production exceed the -E-mail: augusto.schneider@live.com 935 energy obtained through feeding (Butler, 2003). In early postpartum dairy cows, the du...

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The aim of this study was to measure changes in biochemical markers in the peripartum period of primiparous Holstein cows diagnosed with subclinical and clinical mastitis. In this study, 37 dairy cows were monitored daily during milking until 60 days postpartum and were categorized according to the occurrence of clinical mastitis (group mastitis (GM), n = 9) or subclinical mastitis (group subclinical mastitis (GSUB), n = 10) or absence of symptoms (control group (CG), n = 18). Blood samples were collected weekly from -30 to 60 days from calving. Samples were grouped for prepartum (-30 to 0 days from calving), early postpartum (0 to 30 days from calving), and late postpartum (30 to 60 days from calving) periods. Prepartum serum non-esterified fatty acid (NEFA) concentration was higher in GM than in CG (P < 0.01). In addition, CG had higher prepartum serum glucose concentration than GM (P = 0.03). In the early postpartum period, aspartate aminotransferase (AST) activity was lower in CG than in GSUB (P < 0.05), and in the late postpartum period, AST activity was lower in CG than GSUB and GM (P = 0.01). Somatic cell count was higher during the early and late postpartum periods for GM and GSUB when compared to CG (P < 0.01). In this study, primiparous cows with low glucose and higher NEFA in the prepartum were more susceptible for mastitis in the early postpartum, probably due to low immune function associated to a more negative energy balance. In sum, increased prepartum serum NEFA concentration and decreased glucose in primiparous cows were associated with clinical mastitis incidence in the postpartum period.

RESUMO -O objetivo neste estudo foi avaliar o custo de produção e a resposta econômica da utilização de casca de soja (CS) associada a duas fontes de nitrogênio não-proteico (NNP) na terminação de cordeiros em confinamento. Foram utilizados 80 cordeiros mestiços Suffolk, com idade inicial de 69 ± 6 dias e peso vivo inicial de 23,11 ± 1,78 kg. Os animais foram distribuídos em quatro grupos, cada um com uma dieta formulada com uma fonte de NNP: grupo ureia convencional, na proporção de 1%; grupo ureia protegida, na proporção de 1%; grupo ureia protegida convencional, na proporção de 1% + 0,5% de ureia convencional, da dieta total, respectivamente; e grupo controle, sem fonte adicional de NNP. O custo total de produção do cordeiro até a desmama foi de R$ 85,27/animal. Entre os componentes desse custo, a categoria de ovelhas foi a principal contribuinte, com 68,55%, seguida pelos custos referentes ao próprio cordeiro (0-67 dias), que representaram 25,57%, e a dos carneiros, com participação de apenas 5,88%. O custo total médio da terminação em confinamento foi de R$ 57,73/cordeiro. Economic results of feedlot lamb using soybean husks associated with different non-protean nitrogen sourcesABSTRACT -The aim of this study was to evaluate the production costs and economic return of soybean husks (CS) associated with two non-protein nitrogen (NNP) sources, in feedlot finishing lamb. Eighty (80) Suffolk cross lambs, averaging initial age of 79 ± 6 days and initial live weight of 23.11 ± 1.78 kg were used. The animals were grouped in four experimental lots accordingly to NNP source: conventional urea group (CUG, n = 20), which received 1% of common urea; protected urea group (PUG, n = 20), which received 1% of protected urea, at the ratio of 1% + 0.5% conventional urea, of total diet, respectively; and control group, with no additional source of NNP. Total production cost (CT) of lambs at weaning was R$ 85.27/animal. In this cost ewes contributed with 68.55%, followed by the lamb cost (0-67 days), which represented 25.57% and rams with only 5.88%.The average CT in feedlot finishing was of R$ 57.73/lamb. Effective operational cost (COEf) represented R$ 57.35 or 99.33%of CT finishing. Variables of higher representativeness of COEf were: ration (61.56%) and taxes (31.06%). Diets with higher positive gross margin (MB)/lamb were GUC and CG (R$ 1.77 and R$ 44.35), respectively. All diets presented negative net margin (ML) and economic return (RE). In finishing systems of feedlot lamb, the use of diets with no additional source of non-protein nitrogen is the option that allows better economic and productivity results.

In this study we evaluated the effects of Bacillus cereus var. Toyoi and Saccharomyces boulardii on the immune response of lambs to Escherichia coli K88ab and Bovine Herpes Virus type 5 (BoHV-5) vaccines. Thirty, 3-month-old lambs were randomly grouped in three lots of 10 each and vaccinated at days 0 and 30 of the experiment. They grazed on the same pasture and were fed ad libitum twice a day with commercial sheep feed supplemented with either B. cereus var. Toyoi at a concentration of 1 )10 6 viable spores gr (1 , S. boulardii at a concentration of 1 )10 6 CFU gr (1 , or non-supplemented feed. Blood samples were collected at weekly intervals over eight weeks and antibody titres were analysed by ELISA. The mean seroconversions against E. coli and BoHV-5 of the fed probiotics groups were higher (p B0.001) than the controls. Both probiotics enhanced the humoral immune response of lambs to the vaccines.