Characterization of single nucleotide polymorphisms in the promoter region of the bovine paraoxonase 1 (PON1) gene affecting serum enzyme activity in dairy cows

Silveira, Pedro Augusto Silva; Schwegler, Elizabeth; Montagner, Paula; Krause, Ana Rita Tavares; Acosta, Diego Andres Velasco; Halfen, J.; Garlet, Tiago; Barros, Carlos Castilho; Corrêa, Márcio Nunes; Schneider, Augusto

doi:10.1016/j.tvjl.2015.04.028

Cited by 10 publications

(11 citation statements)

References 9 publications

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“…Phosphorus concentration did not change between groups; this could indicate that the parasites affecting respiratory system (D. viviparus) in our study conditions would not produce enough respiratory dysfunction for inducing changes in phosphorus due to respiratory alkalosis. No changes were detected in PON-1 between the groups; indicating that despite the influence that inflammation has in PON-1 (Silveira et al, 2015) in our study this enzyme has shown a lower sensitivity to detect inflammation produced by the parasites studied in calves compared to Hp. Ideally individual experimental infections with different concentrations of each of the parasites found could be made in order to properly characterize the biochemical effects produced by each species of parasites, as well the influence of the parasite burden in analytes.…”

Section: Tablecontrasting

confidence: 69%

Changes in biochemical analytes in calves infected by nematode parasites in field conditions

Cezaro

Tvarijonaviciute

Tecles

et al. 2016

Veterinary Parasitology

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Section: Tablecontrasting

confidence: 69%

Changes in biochemical analytes in calves infected by nematode parasites in field conditions

Cezaro

Tvarijonaviciute

Tecles

et al. 2016

Veterinary Parasitology

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“…Distinct levels of enzymatic activity may be genetically driven by polymorphisms in the promoting and encoding regions of the PON1 gene, as observed in humans (Camps, Marsillach, & Joven, 2009;Padungtod et al, 1999) and in cows (Silveira et al, 2015). Distinct levels of enzymatic activity may be genetically driven by polymorphisms in the promoting and encoding regions of the PON1 gene, as observed in humans (Camps, Marsillach, & Joven, 2009;Padungtod et al, 1999) and in cows (Silveira et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…The expression of the three paraoxanases was confirmed in all samples by the amplification of the gene GAPDH, and the specificity of the amplified fragments was confirmed by both the size of the amplicons estimated through the comparison with the DNA marker and by the inexistence of unspecific bands. Distinct levels of enzymatic activity may be genetically driven by polymorphisms in the promoting and encoding regions of the PON1 gene, as observed in humans (Camps, Marsillach, & Joven, 2009;Padungtod et al, 1999) and in cows (Silveira et al, 2015). The PON1 55M, PON1 192R and PON2…”

Section: Discussionmentioning

confidence: 99%

Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls

et al. 2017

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The paraoxonases types 1, 2 and 3 (PON1, PON2 and PON3, respectively) are enzymes that degrade lipid peroxides, preventing oxidative damages relevant for male reproductive function. This study determined the expression of those three paraoxonases in reproductive tissues of bulls and evaluated correlations among the activity of PON1 in the serum and seminal plasma with breeding soundness parameters in bulls. The expression of PON1, PON2 and PON3 was characterised by RT-PCR in samples of testicular parenchyma, vesicular glands and epididymis collected from three slaughtered bulls. All three paraoxonases were expressed in the testicular parenchyma, PON2 and PON3 were both expressed in the epididymis head and PON3 was also expressed in the epididymis tail. The PON1 activity was determined in samples of serum and seminal plasma from 110 bulls submitted to breeding soundness evaluation. There was a strong correlation (r = .90) between the activity of the PON1 in both serum and seminal plasma (p < .0001). The PON1 activity in the seminal plasma was positively correlated with ejaculate's colour, sperm mass activity (p = .04), motility, vigour and viability (all p < .01). Thus, PON1 may be a potential marker for sperm motility and viability in bulls.

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“…Nevertheless, both in humans as in some terrestrial mammals, PON1 gene polymorphisms highly influence the enzymatic activity toward different substrate: the single-nucleotide polymorphisms (SNPs) Leu55Met and Gln192Arg increase the paraoxonase activity (66) in humans and different PON1 genotypes influence activities toward paraoxon and phenyl-acetate in rabbit (67). Also in cows, some SNPs in the promotor region of PON1 gene are associated with serum PON1 activity (68). Recently, a phylogenetic study on convergent functional losses across marine mammals, has identified a PON1 functional loss in marine mammals, probably related to their different lipid metabolism and fatty acid oxidation due to adaptation to the marine environment and a high concentration of ω-3 fatty acids on their diet.…”

Section: Acute Phase Protein In Marine Mammals: Challenges and Futurementioning

confidence: 99%

Acute Phase Proteins in Marine Mammals: State of Art, Perspectives and Challenges

Gelain

Bonsembiante

2019

Front. Immunol.

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The term “acute phase response” (APR) is referred to a nonspecific and complex reaction of an organism that occurs shortly after any tissue damage, such as infection, trauma, neoplasia, inflammation, and stress. The APR can be identified and monitored with some laboratory tests, such as the concentration of several plasma proteins, the acute phase proteins (APPs). The APPs are components of the non-specific innate immune response, and their plasma concentration is proportional to the severity and/or the extent of tissue damage. The evaluation of health status of marine mammals is difficult because the classical clinical signs of illness used for human and domestic animals are difficult to recognize and understand. For this reason, in the past years, several efforts were done to identify laboratory markers of disease in these animals. The APPs have demonstrated their role as early markers of inflammation in veterinary medicine, thus several APPs were tested in marine mammals, such as C-reactive protein (CRP), serum amyloid-A (SAA), and Haptoglobin (Hp). However, the difficulty to extrapolate the knowledge about APPs in one species to another, the lack of specie-specific reagents, the absence of data about negative APPs have hampered their extent use in marine mammals. Herein, the state of art of APPs in marine mammals is reviewed, with particular attention to pre-analytical and analytical factors that should be taken into account in validation and interpretation of APPs assays. Moreover, the current application, potential utility and the future developments of APPs in marine mammals is highlighted and discussed.

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Characterization of single nucleotide polymorphisms in the promoter region of the bovine paraoxonase 1 (PON1) gene affecting serum enzyme activity in dairy cows

Cited by 10 publications

References 9 publications

Changes in biochemical analytes in calves infected by nematode parasites in field conditions

Changes in biochemical analytes in calves infected by nematode parasites in field conditions

Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls

Acute Phase Proteins in Marine Mammals: State of Art, Perspectives and Challenges

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