Effect of<i>Bacillus cereus</i>var. Toyoi and<i>Saccharomyces boulardii</i>on the immune response of sheep to vaccines

Roos, Talita Bandeira; Tabeleão, Vinícius Coitinho; Dummer, Luana Alves; Schwegler, Elizabeth; Goulart, Maikel Alan; Moura, Sandra Vieira de; Corrêa, M. N.; Gil-Turnes, Carlos

doi:10.1080/09540100903443691

Cited by 24 publications

(8 citation statements)

References 12 publications

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“…Another important property of probiotics is their capacity to modulate immune responses. Some experiments showed that probiotics have immunomodulatory effects depending on the characteristics of the immunogen [ 28 – 30 ] and the adjuvant used in the vaccines [ 31 ]. The effect of probiotics on the enhancement of the titers of specific and natural antibodies in the serum and gut of chickens against the tetanus toxin was already reported [ 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Probiotic effect of Pichia pastoris X-33 produced in parboiled rice effluent and YPD medium on broiler chickens

Santos

Gil-Turnes

et al. 2018

PLoS ONE

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In a previous paper we showed that the yeast Pichia pastoris X-33 grown in parboiled rice effluent supplemented with glycerol byproduct from the biodiesel industry improved the quality of the effluent. In this paper we show the validation of this yeast (PPE) as probiotic for broilers. Its effect on feed efficiency and immunomodulation was compared with the same yeast grown in yeast peptone dextrose medium (PPY), with Saccharomyces boulardii (SBY) and with the controls fed unsupplemented feed (CON). One-day-old female chicks were vaccinated against infectious bursal disease (IBD) and the titers of anti-IBD antibodies were measured by ELISA. PPE group had the highest mean titres on days 14 and 28 (p<0,05), and at 28 days, 64% of the animals showed seroconvertion. The PPE group also showed the best weight gains at 42 days of age, that, on days 7, 14 and 21 were 19%, 15%, and 8.7% higher, respectively, than the control group. The best feed conversion, 8.2% higher than the control group, was obtained by PPY at 42 days. Histopathological studies did not detect any undesirable effects in the supplemented animals. We concluded that Pichia pastoris X-33 when grown in effluents of the rice parboiling industry supplemented with glycerol byproduct from the biodiesel has probiotic properties for poultry.

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Section: Discussionmentioning

confidence: 99%

Probiotic effect of Pichia pastoris X-33 produced in parboiled rice effluent and YPD medium on broiler chickens

Santos

Gil-Turnes

et al. 2018

PLoS ONE

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“…Rodrigues et al [ 29 ] reported that with the aid of such tools, they designed new versions of the rCPB protein and produced an unpurified protein (rCPB-C) with high productivity, solubility and antigenicity, which is another promising option for the development of recombinant vaccines that induce a more lasting humoral immune response. Probiotics can act as immunomodulators and enhancers of the immune response of animals after vaccination [ 30 , 31 , 32 ]. In a recent study, ewes immunized with a formulation containing the rETX protein of C. perfringens , after supplementation with Bacillus toyonensis BCT-7112T, showed a 2- to 3-fold increase in total serum levels of anti-rETX IgG compared to the group of no supplemented animals [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Measurement over 1 Year of Neutralizing Antibodies in Cattle Immunized with Trivalent Vaccines Recombinant Alpha, Beta and Epsilon of Clostridium perfringens

Galvão

Barbosa

Oliveira

et al. 2021

Toxins

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The alpha (CPA), beta (CPB) and epsilon (ETX) toxins of Clostridium perfringens are responsible for causing diseases that are difficult to eradicate and have lethal potential in production animals. Vaccination of herds is still the best control strategy. Recombinant clostridial vaccines have shown good success at inducing neutralizing antibody titers and appear to be a viable alternative to the conventional production of commercial clostridial toxoids. Research is still needed on the longevity of the humoral immune response induced by recombinant proteins in immunized animals, preferably in target species. The objective of this study was to measure the humoral immune response of cattle immunized with trivalent vaccines containing the recombinant proteins alpha (rCPA), beta (rCPB) and epsilon (rETX) of C. perfringens produced in Escherichia coli at three different concentrations (100, 200, and 400 µg) of each protein for 12 months. The recombinant vaccines containing 200 (RV2) and 400 µg (RV3) yielded statistically similar results at 56 days. They performed better throughout the study period because they induced higher neutralizing antibody titers and were detectable for up to 150 and 180 days, respectively. Regarding industrial-scale production, RV2 would be the most economical and viable formulation as it achieved results similar to RV3 at half the concentration of recombinant proteins in its formulation. However, none of the vaccines tested induced the production of detectable antibody titers on day 365 of the experiment, the time of revaccination typically recommended in vaccination protocols. Thus, reiterating the need for research in the field of vaccinology to achieve greater longevity of the humoral immune response against these clostridial toxins in animals, in addition to the need to discuss the vaccine schedules and protocols adopted in cattle production.

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“…In neonate calves supplemented with Lactobacillus acidophilus and L. plantarum or L. plantarum only, a significantly slower decrease in serum IgG was observed compared to the control group 83 . The yeast probiotic Saccharomyces boulardii at 10 6 and 10 7 CFU/g in the supplemented feed enhanced blood IgG in lambs following a vaccine against BoHV-5 virus and Escherichia coli, thus enhancing the humoral immune response 84,85 . On the contrary, supplementation of L. plantarum to dairy goats did not impact blood IgG, IgM, and IgA concentrations 86 .…”

Section: The Effect Of Live Yeast Supplementation In Late Gestation On Colostrum and Hypothesis On Mechanisms Involved In Colostrum Qualimentioning

confidence: 99%

A live yeast supplementation to gestating ewes improves bioactive molecules composition in colostrum with no impact on its bacterial composition and beneficially affects immune status of the offspring

Dunière

Renaud

Steele

et al. 2021

Preprint

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Colostrum quality is of paramount importance in the management of optimal ruminant growth and infectious disease prevention in early life. Live yeast supplementation effect during the last month of gestation was evaluated on ewes colostrum composition. Two groups of ewes (n=14) carrying twin lambs were constituted and twins were separated into groups (mothered or artificially-fed) 12h after birth. Nutrient, oligosaccharides (OS), IgG and lactoferrin concentrations were measured over 72h after lambing, and bacterial community was described in colostrum collected at parturition (T0). Immune passive transfer was evaluated through IgG measurement in lamb serum. In both groups, colostral nutrient, OS concentrations and IgG concentrations in colostrum and lamb serum decreased over time, (p < 0.01) except for lactose, which slightly increased (p < 0.001) and lactoferrin which remained stable. Bacterial population was stable over time with high relative abundances of Aerococcaceae, Corynebacteriaceae, Moraxellaceae and Staphylococcaceae in T0-colostrum. No effect of supplementation was observed in nutrient and lactoferrin concentrations. In supplemented ewes, colostral IgG level was higher at T0 and a higher level of serum IgG was observed in lambs born from supplemented mothers and artificially-fed, while no effect of supplementation was observed in the mothered lambs groups. Using a metabolomic approach, we showed that supplementation affected OS composition with significantly higher levels of colostral Neu-5Gc compounds up to 5h after birth. No effect of supplementation was observed on bacterial composition. Our data suggest that live yeast supplementation offsets the negative impact of early separation and incomplete colostrum feeding in neonate lambs.

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Effect ofBacillus cereusvar. Toyoi andSaccharomyces boulardiion the immune response of sheep to vaccines

Cited by 24 publications

References 12 publications

Probiotic effect of Pichia pastoris X-33 produced in parboiled rice effluent and YPD medium on broiler chickens

Probiotic effect of Pichia pastoris X-33 produced in parboiled rice effluent and YPD medium on broiler chickens

Measurement over 1 Year of Neutralizing Antibodies in Cattle Immunized with Trivalent Vaccines Recombinant Alpha, Beta and Epsilon of Clostridium perfringens

A live yeast supplementation to gestating ewes improves bioactive molecules composition in colostrum with no impact on its bacterial composition and beneficially affects immune status of the offspring

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