In emotional research, efficient designs often rely on successful emotion induction. For visual stimulation, the only reliable database available so far is the International Affective Picture System (IAPS). However, extensive use of these stimuli lowers the impact of the images by increasing the knowledge that participants have of them. Moreover, the limited number of pictures for specific themes in the IAPS database is a concern for studies centered on a specific emotion thematic and for designs requiring a lot of trials from the same kind (e.g., EEG recordings). Thus, in the present article, we present a new database of 730 pictures, the Geneva Affective PicturE Database, which was created to increase the availability of visual emotion stimuli. Four specific negative contents were chosen: spiders, snakes, and scenes that induce emotions related to the violation of moral and legal norms (human rights violation or animal mistreatment). Positive and neutral pictures were also included: Positive pictures represent mainly human and animal babies as well as nature sceneries, whereas neutral pictures mainly depict inanimate objects. The pictures were rated according to valence, arousal, and the congruence of the represented scene with internal (moral) and external (legal) norms. The constitution of the database and the results of the picture ratings are presented.

One of the central tenets of emotion theory is that emotions involve coordinated changes across experiential, behavioral, and physiological response domains. Surprisingly little is known, however, on how the strength of this emotion coherence is altered when people try to regulate their emotions. To address this issue, we recorded experiential, behavioral, and physiological responses while participants watched negative and positive pictures. Cross-correlations were used to quantify emotion coherence. Study 1 tested how two types of suppression (expressive and physiological) influence coherence. Results showed that both strategies decreased the response coherence measured in negative and positive contexts. Study 2 tested how multi-channel suppression (simultaneously targeting expressive and physiological responses) and acceptance influence emotion coherence. Results again showed that suppression decreased coherence. By contrast, acceptance was not significantly different from the unregulated condition. These findings help to clarify the nature of emotion response coherence by showing how different forms of emotion regulation may differentially affect it.

This study examines the early affective consequences of two close forms of suppression. Participants (N=37) were shown negative, positive, and neutral pictures and cued either to attend to the pictures, or to perform expressive or physiological suppression (i.e. reduce body reactions). Continuous measures of experience, expressivity, and autonomic responses showed that both suppression strategies produced rapid response modulation. Common effects of the two strategies included a transient increase in negative feeling, a durable decrease in positive feeling, and a decrease in expressivity, cardiovascular activity, and oxygenation. The two strategies were significantly different only in response to positive stimuli, with physiological suppression showing a larger decrease in experience intensity and blood pressure. These results suggest a strong overlap between the two suppression strategies in terms of their early impact on emotional responses.

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Popular opinion holds that color has specific affective meaning. Brighter, more chromatic, and warm colors were conceptually linked to positive stimuli and darker, less chromatic, and cool colors to negative stimuli. Whether such systematic color associations exist with actually mood felt remains to be tested. We experimentally induced four moods—joy, relaxation, fear, and sadness—in a between‐subject design (N = 96). Subsequently, we asked participants to select a color, from an unrestricted sample, best representing their current mood. Color choices differed between moods on hue, lightness, and chroma. Yellow hues were systematically associated with joy while yellow‐green hues with relaxation. Lighter colors were matched to joy and relaxation (positive moods) than fear and sadness (negative moods). Most chromatic colors were matched to joy, then relaxation, fear, and sadness. We conclude that color choices represent felt mood to some extent, after accounting for a relatively low specificity for color‐mood associations.

Subjective feeling, defined as the conscious experience of emotion and measured by self-report, is generally used as a manipulation check in studying emotional processes, rather than being the primary focus of research. In this paper, we report a first investigation into the processes involved in the emergence of a subjective feeling. We hypothesized that the oscillatory brain activity presumed to underlie the emergence of a subjective feeling can be measured by electroencephalographic (EEG) frequency band activity, similar to what has been shown in the literature for the conscious representation of objects. Emotional reactions were induced in participants using static visual stimuli. Episodes for which participants reported a subjective feeling were compared to those that did not lead to a conscious emotional experience, in order to identify potential differences between these two kinds of reactions at the oscillatory level. Discrete wavelet transforms of the EEG signal in gamma (31-63 Hz) and beta (15-31 Hz) bands showed significant differences between these two types of reactions. In addition, whereas beta band activities were widely distributed, differences in gamma band activity were predominantly observed in the frontal and prefrontal regions. The results are interpreted and discussed in terms of the complexity of the processes required to perform the affective monitoring task. It is suggested that future work on coherent mental representation of multimodal reaction patterns leading to the emergence of conscious emotional experience should include modifications in the time window examined and an extension of the frequency range to be considered.

Most human genes contain multiple alternative splice sites believed to extend the complexity and diversity of the proteome. However, little is known about how interactions among alternative exons regulate protein function. We used the Caenorhabditis elegans slo-1 large-conductance calcium and voltage-activated potassium (BK) channel gene, which contains three alternative splice sites (A, B, and C) and encodes at least 12 splice variants, to investigate the functional consequences of alternative splicing. These splice sites enable the insertion of exons encoding part of the regulator of K + conductance (RCK)1 Ca 2+ coordination domain (exons A1 and A2) and portions of the RCK1-RCK2 linker (exons B0, B1, B2, C0, and C1). Exons A1 and A2 are used in a mutually exclusive manner and are 67% identical. The other exons can extend the RCK1-RCK2 linker by up to 41 residues. Electrophysiological recordings of all isoforms show that the A1 and A2 exons regulate activation kinetics and Ca 2+ sensitivity, but only if alternate exons are inserted at site B or C. Thus, RCK1 interacts with the RCK1-RCK2 linker, and the effect of exon variation on gating depends on the combination of alternate exons present in each isoform.Slo1 channels | regulator of K conductance domains | C. elegans A lternative splicing is observed in all eukaryotic organisms and diversifies the proteome encoded by metazoan genomes while preserving their compact sizes. Most human genes (90%) encode multiple transcripts (1, 2), and on average, each multiexon gene encodes at least seven distinct transcripts (1). Although alternative splicing is known to change protein function by adding, removing, or altering functional domains (3, 4), the combinatorial effects of alternative splicing across multiple sites on protein function have not been examined previously. To address these questions, we examined the functional differences among isoforms of the slo1 gene that is conserved among metazoans and subject to extensive alternative splicing (5, 6). The slo1 gene in Drosophila and its ortholog in humans, KCNMA1, encode 10 and 13 alternate exons, respectively (5). If all possible exon combinations were to be expressed, then each of these genes would encode more than 1,000 transcripts. By contrast, the Caenorhabditis elegans slo-1 gene is much simpler, with three alternate splice sites (7) and 12 possible splice variants. Thus, the C. elegans slo-1 gene offers an opportunity for a comprehensive analysis of the functional diversity of splice variants generated by a single gene that is conserved from worms to humans.slo1 encodes the pore-forming subunit of large-conductance, Ca 2+ -and voltage-activated K + (BK) channels. Accordingly, functional changes caused by alternative splicing are readily measured using the patch-clamp technique. BK channels regulate cellular excitability by linking Ca 2+ signaling to membrane repolarization and are essential for vascular tone (8-10), endocrine secretion (11, 12), neurotransmitter release (13, 14), and frequency tuning in hair ce...