This paper investigated the effect of cryoprotectant substances on Prochilodus lineatus embryos in an experimental incubator. The prospective study applied combinations of polyvinyl alcohol, hydroxyethyl cellulose, gelatin and fetal bovine serum with dimethyl sulfoxide and ethylene glycol in a new experimental incubator. The morphology of embryos, larval viability and the efficiency of experimental incubators in maintaining the quality of embryos were evaluated. This study demonstrates the efficient association between hydroxyethylcellulose and dimethyl sulfoxide as greater viability (p<0.05) was found for embryos (72.9 ± 23.9%). It should also be noted the permeation of cryoprotectants in embryos through the changes found in chorion diameter, embryo diameter and embryo volume comparing the treatments versus control group (water) (p<0.05), this results can help in future cryopreservation protocols. Although the temperature and oxygenation differed between the usual and experimental incubators (p<0.05), the results showed a high fertilization rate (79.6 ± 13.2%) for experimental incubators (p<0.05) which is sufficient for the maintenance of embryos in a cryoprotective environment and effectively allows experimentation for long periods with cryoprotectant substances. Cryopreservation of fish embryos has not been accomplished yet and new approaches are required for understanding the permeability of teleost embryos, especially in Brazilian native species.Este trabalho avaliou o efeito de substâncias crioprotetoras sobre embriões de Prochilodus lineatus em uma incubadora experimental. O estudo aplicou combinações de álcool polivinílico, hidroxietilcelulose, gelatina e soro fetal bovino com dimetilosulfóxido e etilenoglicol em uma nova incubadora experimental. Foram avaliadas a morfologia dos embriões, a viabilidade larval e a eficiência das incubadoras experimentais na manutenção da qualidade dos embriões. Este estudo demonstra a associação eficiente entre hidroxietilcelulose e dimetilsulfóxido pela maior viabilidade (p<0,05) encontrada para os embriões (72,9 ± 23,9%). Deve-se notar também a permeação dos crioprotetores nos embriões através das alterações encontradas no diâmetro córion, diâmetro do embrião e no volume do embrião comparando os tratamentos ao grupo controle (água) (p<0,05), estes resultados podem ajudar em futuros protocolos de criopreservação. Embora a temperatura e a oxigenação diferiram entre as incubadoras comuns e as experimentais (p<0,05), os resultados mostraram elevada taxa de fertilização (79,6 ± 13,2%) para incubadoras experimentais (p<0,05), o que é suficiente para a manutenção de embriões em ambiente crioprotetor e permite efetivamente a experimentação por longos períodos com substâncias crioprotetoras. A criopreservação de embriões de peixes ainda não foi realizada e novas abordagens são necessárias para a compreensão da permeabilidade dos embriões de teleósteos, especialmente em espécies nativas brasileiras.

This study determined the age and its correlation with biometric variables and reproductive indices of piapara Leporinus obtusidens caught at the Funil Reservoir. We caught 133 specimens between September 2006 and August 2007. Weight, total length, standard length, depth, height, head length and radius of the scales were measured. The sex was determined by macroscopic examination of gonads. The age of each fish was determined by analyzing the growth rings on the scales. Seasonal differences in biometric variables were tested using the NK test at 5%. The correlation of age and radius of the scales with the biometric variables and reproductive indices were analyzed using the SAEG software. Fish individuals ranging from 3 to 14 years of age were caught. In the spring and summer, smaller fish were captured compared to those caught during the fall and winter. Females tended to have higher weight and morphometric values when compared to males. Age and radius of the scales were correlated with biometric variables in L. obtusidens. It can be concluded that the biometric variables of this species can be used as an indicator of age.

The aim of this study was to evaluate morphometric and hematological parameters of juveniles Piracanjubas treated with two distinct photoperiod. Twenty nine fish with 100 g, in a factorial design were performed in 18 h light:6 h dark (18L:6D) and 6L:18D two different photoperiod, and morphometric and hematological parameters were evaluated at 45 d and 90 d after photoperiod treatment. Benzocaine 1% was used for euthanasia. Fish were weighed and measured, so as visceral fat, liver and gonads. Hematocrit, total count of white cells, lymphocytes and neutrophils were counted. Results showed that there was no significant relationship between bodyweight and standard length. Significant interaction (P = 0.00018) had been found between the fat index (FI) and gonado-somatic index (GSI) and the photoperiod and time. At 90 d, the fish kept in 18L:6D had a lower rate of fat than the fish kept in 6L:18D (P < 0.05). The GSI was higher in the group 6L:18D compared with the 18L:6D (P < 0.005). There was no interaction between photoperiod and period of treatment for total leukocyte count, however with 90 d, the total leukocyte count was decreased (P < 0.05). Moreover, decrease of the hematocrit from 58.1% to 46.9% and an increase of lymphocytes from 31.5% to 43.5% had been found in fish kept in 18L:6D after 90 d (P < 0.05). Fish kept in 6L:18D presented an increase in segmented neutrophils and lymphocytes (P < 0.05). It is concluded that photoperiod maintained at 6L:18D for Piracanjubas permits a greater gonadal development and an amount of immune cells over the photoperiod maintained in 18L: 6D.

A prevalência de discromatopsia congênita (daltonismo) entre os homens é de aproximadamente 6% a 10%. No curso de medicina, as pessoas afetadas apresentam problemas na diferenciação entre estruturas histológicas. Nesse aspecto, foi desenvolvido na Faculdade de Medicina de Itajubá (FMIT) um trabalho preliminar de adaptação das aulas práticas de histologia para um estudante daltônico, que apresentou baixo rendimento na primeira avaliação. Assim, quando observado o problema, o docente buscou compreender as cores que o estudante apresentava dificuldades, permitindo-o adaptar as aulas para incluir seu espectro de cores. Esta inclusão foi realizada dando maior foco nas

RESUMOO presente resumo expandido apresenta o êxito da utilização de mapas conceituais como ferramenta aliada da Metodologia Ativa (MA), sobretudo no ensino de assuntos inerentes ao ciclo básico da Medicina. Diante disso, o projeto de extensão Conceitual Med, da Faculdade de Medicina de Itajubá, alcançou eficácia na aplicação desse mecanismo.