To create recommendations regarding the technological process of growing objects of warm-water aquaculture, the health of individuals in the created conditions of maintain is a necessary study. Taking into account the similarity of the necessary conditions for the organization of growing warm-water aquaculture objects, it is important to monitor the indicators that are bioindicators of homeostasis constants with a projection on the conditions of maintain, for subsequent adjustment of satisfactory conditions of maintain. First of all, these are hematological and biochemical bioindicators that determine the general characteristics of blood in the case of fish objects of sterlet and hemolymph in the case of crustacean objects of Australian freshwater crayfish and the concentration index of total serum protein that determines the “physiological norm”. When growing sterlet, a high level of total blood protein was established at 40.14±1.73 g/l and the hemolymph of the Australian freshwater crayfish was determined to have a protein concentration of 40.8±4.5 g/l. The presented analysis of bioindicators complements the missing information for monitoring the data of representatives of warm-water aquaculture, which will be valuable for specialists engaged in breeding these objects.
The article describes Australian red claw crayfish, Cherax quadricarinatus, which is currently one of the most promising cultivation targets. However, to increase production volumes, it is necessary to improve cultivation technologies, which should be based on the data of physiological and biochemical parameters. An objective method for monitoring the physiological state of Australian red claw crayfish in the artificial conditions is determining its hematological and biochemical indicators that complement the general characteristics of hemolymph and later define its physiological norm. When giving the objects the compound feed, which the authors produced by their own recipe, there was recorded a high level of total hemolymph protein of 40.8 ± 4.5 g/l. According to the results of biochemical research, the cholesterol content in the hemolymph of Australian crayfish was 3.2 ± 0.6 mmol/l, and the concentration of lipoproteins was 0.8 ± 0.2 g/l (levels within the reference values). When considering the hemogram of hemolymph there were found the dominant types and linear dimensions of native formed elements. The diameter of agranulocytes in Australian freshwater crayfish varied from 85 to 90 μm, semi-granulocytes - from 90 to 95 μm, granulocytes - from 65 to 78 μm, transparent cells differed by greater variability in size, which ranged from 85 to 120 μm. The part of granulocytes in relation to other shaped elements exceeded 50%, for transparent cells it turned out to be above 20%, for semi-granulocytes - about 15% and for agranulocytes - about 5%, which means that the latter form the rarest group. The established ratios are specific for Cherax quadricarinatus, its hemocytic characteristics greatly differs from Astacus leptodactylus, which is the main object of comparative research. The results of comparative analysis of the cellular composition of the hemolymph of the Australian freshwater crayfish Cherax quadricarinatus (Von Martens, 1868) demonstrate the taxonomic specificity of this representative of freshwater aquaculture
The objects of research are Beluga (Huso huso, Linnaeus), sturgeon spike (Acipenser nudiventris, Lovetsky) and their interspecific hybrids of artificial generation grown on the Research and Experimental Base BIOS (the Astrakhan region) by using the basin method. To assess the physiological status of fish, a set of morphophysiological indicators (absolute and relative weight of gills, heart, liver, and gonads) were used. The dynamics of the indices of the corresponding viscera of Beluga and sturgeon spike and their interspecific hybrids in the second year of cultivation was studied. The necessity for the research is explained by the fact that most work performed was carried out on land vertebrates and on fish of natural generation. There have been presented the average values of morphophysiological indices for each of the four groups of unmixed sturgeon species and their hybrids. The obtained values of the studied parameters of sturgeon species can be used for further monitoring of the fish growing conditions using the basin method.
The authors of this article describe the experience of biotechnology for growing Australian crayfish in conditions of pond cultivation in a group of ponds, operating in an intensive mode, used in an isolated area close by a natural water source in the Astrakhan region. In the presented article, research is aimed to clarify the main biotechnological indicators that increase the efficiency of cultivation in pond conditions. The conditions of transportation, the duration of transportation, and the density of crayfish in ponds with an area of 1 hectare have been clarified. The presented food recipe with the specification of the percentage of nutrients as additional feeding, organized when growing in ponds according to the food recipe itself, increases not only the growth rate but also the hemolymph protein concentration, which indirectly proves the satisfactory conditions for keeping aquaculture objects. Monitoring for the biotechnology of growing Australian crayfish in pond conditions: own developed food recipe, biological and physiological testing of key indicators such as hydrochemical indicators of water, hydrobiological indicators of the concentration of, and physiological indicators for determining the so-called biotechnological norm, which will add additional missing information on this aquaculture object.
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