SUMMARY:This study describes the preparation and use of Roselle (Hibiscus sabddariffa) for the differential staining of testicular tissue sections to find out its staining effect on nuclear, cytoplasmic and other structures. Various treatments using modifications of the plant extract in water were carried out on sections of the rabbit testis. Various levels of differentiation of nuclear and cytoplasmic structures as well as other structures of this organ was obtained especially when 1% eosin was applied as a counter stain. The best staining result was obtained when iron alum was used to mordant the extract and when the extract, mordanted with potassium alum was acidified using acetic acid and used to stain the sections. Modification of the aqueous extract to an alkaline pH using ammonia gave the poorest staining effect. Roselle extract therefore shows reasonable potential as a candidate nuclear stain especially when modanted with iron alum or mordanted with potassium alum and acidified with acetic acid.
Objective: To measure the transcript levels of Aurora kinases and compare them to their immunoreactivity patterns in prostate tumors. Materials and Methods: A total of 26 cases of prostate cancer (PCa) and 38 cases of benign nodular hyperplasia (BPH) were sampled from archived formalin-fixed paraffin-embedded tissues. Tissue sections were lysed, total RNA extracted and cDNA made by random hexamer priming while slide sections were immunostained for the kinases. Normalized relative quantitation was performed for all the kinases using real-time PCR on TaqMan chemistry. Results: The immunoreactivity profile showed 15.4, 53.8 and 30.7% positivity for Aurora kinases A, B and C in PCa cases, respectively, while the positivity was 76.3, 73.7 and 84.2% for the same kinases in BPH cases. The immunoreactivity was preponderant on epithelial tissue compared to stromal component. Conclusion: Aurora kinases were significantly overexpressed in BPH cases compared to PCa cases. At the transcript level, there was no significant differential expression in the kinases between PCa and BPH cases.
IntroductionDiagnosis of prostatic diseases with Immunohistochemistry still faces challenges because of the peculiar histology of the prostate and difference(s) in reactivity of Monoclonal antibodies (MoAb) to benign and malignant changes.MethodsThirty (30) archived paraffin embedded tissue samples from primary prostate tumors (15 Benign Prostatic Hyperplasia (BPH) and 15 Cancer of the prostate (CaP)) were sectioned at thickness of 5µm and confirmed as BPH or CaP. Sections from each sample were stained by Immunohistochemistry using the Streptavidin-biotin method and using CK5/6, CK7, CK8,CK20 and Ki67 antibodies (Zymed Antibody products). Appropriate positive and negative controls for each antibody were setup alongside the test slides.ResultsBPH samples were reactive to Ck5/6 (93.3%), Ck7 (80%) and Ck8 (100%). Only 13.3% of BPH samples were reactive to Ki67. The reactivity of Ck5/6, 7, 8 in CaP is a contrast with only 3(20%) of samples positive with Ck5/6, 2(13.3%) positive with Ck7 and 14(93.3%) with Ck8. While reactivity of Ck 8 is similar in BPH and CaP, no reaction was recorded in Ck 20 in both BPH and CaP. Ki67 was only reactive in 2(13.3) of BPH samples and 15(100%) of CaP. Only Ck 8 was expressed in both BPH and CaP. There was co-expression of Ck5/6, 7,8 and Ki67 in13.3%; Ck7and Ki67 in 13.3% in both BPH and CaP.ConclusionThe various cytokeratins are individually expressed in both BPH and CaP. Ck5/6 and Ck7 are co-expressed and may be used in the diagnosis of BPH, Ck5/6,7,8 and Ki67 are co-expressed in Prostatic adenocarcinoma and squamous cell carcinoma of the prostate while Ck8 and Ki67 are co-expressed and may be used for diagnosis of Prostatic adenocarcinoma alone.
Background:The involvement of communities in control of cervical cancer cannot be overemphasized, but this must take cognizance of their current knowledge, attitudes/beliefs, and practices (KABP) of the people if it will be sustainable. This study assessed the prevalence of cervical intraepithelial neoplasia (CIN) among university students and their level of KABP concerning cervical screening in Maiduguri North-Eastern, Nigeria. Materials and Methods: Two hundred and ifty-two subjects (age range: 18-69 years) were screened using pap smear screening method and acetowhite method. A structured questionnaire was administered on each subject to elicit information on KABP that could predispose them to the disease. Results: CIN was recorded in 12.8% of subjects with low-grade squamous intraepithelial lesion in 10.8% and high-grade squamous intraepithelial lesion 2.0% of the women, respectively. The average general level of knowledge of various aspect of was 43.3% average positive attitudes/beliefs about the disease was recorded in 17.1% of subject, while positive practices that could lead to prevention of the disease was obtained in 30.0%. Conclusion: The level of knowledge of the disease and screening is very low and together with high levels of negative attitudes and practices, will adversely affect control measures and therefore have to be addressed.
Background: The leaves of Lawsonia inermis (henna) is widely used as cosmetic agent for body art to colour the hair, skin and nails, and hence is considered as a candidate stain for tissues. Lawsonia inermis leaves contain a pigment which is the source of red-brown dye. The application of natural dyes for staining biological tissues will reduce cost and is more environmentally friendly.Methods: The natural dye Lawson (2-hydroxy-1,4-napthaquinone) was extracted from the dry leave of Henna using two solvents (distilled water and ethanol). The two extracts were formulated into various staining solutions at different concentrations. The staining solutions were modified with potassium alum and phenol. Liver sections were stained using haematoxylin and eosin method alongside various modifications of the plant extract as counterstain at different timing and temperature conditions.Results: The solutions were found to be acidic in nature and stained the cytoplasm golden brown with a well-defined cytoplasmic boundary of hepatocytes. The best staining effect was obtained with 1% concentration at room temperature for 10 minutes. This provides a measure of contrast between the nuclei, cytoplasm and connective tissue that is adequate for diagnosis. Phenol used as an accentuator has been found to improve the staining but caused swelling of cell. Mordanting with potassium alum did not cause swelling but did not give the kind of resolution as obtained with Phenol. Use of the henna extract at 60oC gave the poorest result which showed that high temperature adversely affect the staining reaction by presenting almost the same color to the structures irrespective of the solvent or concentration.Conclusions: This findings suggest that Henna leave extract can be used as a suitable cytoplasmic connective tissue stain.
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