BackgroundThis study investigates the influence of insulin on pulp tissue healing after pulp capping in diabetic dogs.MethodsDiabetes mellitus was induced in four dogs, and their blood glucose levels were adjusted by insulin to normoglycemic level in two dogs (normoglycemic group) and to hyperglycemic level in two dogs (hyperglycemic group). Class V buccal cavities were performed in 15 teeth in each diabetic dog and two normal dogs (control group). The three groups (30 teeth each) were divided according to the capping materials into three subgroups (10 teeth each) including: subgroup A ‐ mineral trioxide aggregate (MTA), subgroup B ‐ bioaggregate (BA), and subgroup C ‐ calcium hydroxide (Ca(OH)2). Each subgroup was divided into two subdivisions according to the evaluation period, subdivision 1: 1 month and subdivision 2: 2 months. Qualitative and quantitative evaluations of the inflammation and dentine bridge formation were assessed histologically.ResultsThe hyperglycemic diabetic group exhibited significant higher inflammatory cell count and scores and lower dentine bridge thickness than those of the normoglycemic diabetic and control groups (P < .05). There were no significant differences in these parameters between the normoglycemic diabetic and control groups (P > .05).ConclusionInsulin has favourable effects on the pulp tissue healing after pulp capping in diabetic dogs.
Aims and ObjectivesThis study aims to analyze immunocytochemically p53 aberrant expression and mdm-2 expression in primary and recurrent mucoepidermoid carcinoma (MEC) of parotid gland and to ascertain if expression of these markers correlates with tumor behavior, clinical outcome, histological grade and local recurrence.Methods20 cases histologically diagnosed as primary MEC with different grades were included in the study. Out of 20 cases, 7 were classified as grade I, 8 as grade II and 5 as grade III. Immunohistochemical staining of these 20 primary cases as well as 6 recurrent cases with anti-p53 and anti-mdm-2 antibodies was carried out. Area fraction of immunopositivity was estimated by image analysis software.Results16/20 primary cases were p53 +ve (80%). The p53 positive cases included 3 cases classified as grade (I), 8 cases as grade (II) and 5 cases as grade (III). All 6 recurrent cases were p53 +ve. On the other hand, 14/20 primary and only 2/6 recurrent cases were mdm-2 +ve. The mdm-2 +ve primary cases included 2 classified as grade (I), 7 as grade (II) and 5 as grade (III). 12 primary MEC showed co-expression of both p53 and mdm-2 of which 2 cases showed local recurrence.Conclusionsthese data suggested that expression of p53 and mdm-2 in primary and recurrent MEC correlates with the high histological grade. P53 aberrant expression is not only considered as an early event in MEC carcinogenesis but also correlates to tumor behavior and local recurrence. Mdm-2 overexpression is correlated to pathogenesis of MEC. However, no strong evidence was found between mdm-2 expression and MEC local recurrence.
Background: Warthin's tumor is a benign monomorphic adenoma with unclear origin that almost occur exclusively in the parotid gland. Etiology of Warthin's tumor as well as its malignant potential are still unclear. Therefore immunohistochemical assessment of Warthin's tumor may be useful to detect its origin or its malignant transformation potential.
Background: Oral squamous cell carcinoma (OSCC) is one of the most prevalent oral cancers, despite rapid improvement in chemotherapy, radiotherapy, and targeted gene therapy used in conjunction with mainstay surgery, the OSCC prognosis remains low. Previous studies have demonstrated anti-proliferative activity of polyphenols in cancer cells, Pyrogallol (PG) one of natural polyphenols, with cytotoxicity and apoptogenic effects on neoplastic and transformed cells, but not in normal cells. Objective: This study aims to investigate the effect of PG on tongue squamous cell carcinoma SCC-25 cells and evaluate possible apoptotic pathways on SCC-25 cells. Methods: Cytotoxicity was measured by MTT assay. Mode of cell death was determined using Image morphometric analysis and Estimation of caspase-3 by Real Time PCR. Results: MTT results showed that SCC-25 cells were sensitive to PG with an IC 50 value of 19.17 ug/ml. Image morphometric analysis showed a decrease in the mean values of Nuclear Area Factor (NAF) of SCC-25 cells treated with Different concentrations of PG when compared to control cells. In addition, PG induced apoptosis by simultaneous up-regulation of capase-3.Conclusions: Different concentrations of PG have a cytotoxic effect on SCC-25 cells. PG was also found to be an effective anti-carcinogenic agent with less toxicity.
Objective: The present study was conducted to evaluate the effect of soft tissue augmentation using a self-inflating soft tissue expander when performed before horizontal alveolar ridge augmentation on the outcomes of the bone augmentation procedure. The primary outcome is the bucco-palatal radiographical changes in alveolar ridge width, while the secondary outcome is the quality of the augmented bone assessed histomorphometrically. Materials and Methods:Sixteen patients underwent horizontal alveolar ridge augmentation using autogenous bone. For the test group, soft tissue expanders were used in a separate surgery before bone grafting surgery. For the control group, patients received treatment including single surgery of bone grafting associated with periosteal releasing incision. Implants were placed in both groups 6 months after bone augmentation. Bucco-palatal changes in alveolar ridge width were evaluated via cone-beam computed tomography. Augmented bone quality was assessed histomorphometrically.Results: After 6 months, regarding radiographic bone width, there was no statistically significant difference between the two groups, as mean bone width in group I and group II were 8.57 mm and 8.75 mm, respectively. Regarding histomorphometric analysis, Group I showed significantly higher mean bone surface area fraction, higher median mature collagen area fraction, and higher median blood vessel count than Group II (p-value = .012), (p-value = .004), and (p-value = .014), respectively. Conclusion:Within the limitations of the present study, soft tissue expander has no influence on bone width gain after horizontal alveolar ridge augmentation with an autogenous bone block but may have a positive effect on the quality of augmented bone.
Objectives The primary objective was to evaluate clinically the efficacy of modified perforated collagen membrane (PCM) and/or (L-PRF) in combination with xenogeneic block bone graft in the vertical alveolar ridge augmentation. Secondary outcome measurements were to evaluate histologically the quality of newly formed tissues. Materials and Methods Six adult mongrel dogs were enrolled in this randomized blinded study. After defect preparation, xenogeneic screw fixed block graft was covered by occlusive collagen membrane in the group (1) (Block + CM - control group ), while group (2), (L-PRF) membrane was added first before top coverage by occlusive collagen membrane (Block + L-PRF + CM). Groups (3) and (4) were identical to the first two groups except that, the occlusive collagen membrane was replaced by a perforated one, so group (3) was (Block + PCM ) and group (4) was (Block + L-PRF + PCM). Following a healing period of 2 months, dogs were submitted to surgical reentry phase for clinical and histological evaluation. Results Clinically, no significant differences were found among all groups regarding vertical and horizontal ridge dimensions (P = 0.155, 0.492 respectively). Histomorphometric analysis revealed that the percentage of total bone area and mature bone was significantly higher in group 4 (69.36 ± 2.72), (33.11 ± 5.18) compared to control group (59.17 ± 4.27),( 21.94 ± 2.86) (P = 0. 027, P = 0.029). Conclusion The use of xenogenic block grafts in combination with a double-layered perforated collagen-L-PRF membrane in vertical ridge augmentation appeared to improve the inductive power of this challenging defect type. Clinical Relevance: Size and number of perforations may affect the mechanical and handling properties of the membrane.
Objectives This study evaluated clinically and histologically the efficacy of modified perforated collagen membrane (PCM) and/or leukocyte- and platelet-rich fibrin (L-PRF) in combination with xenogeneic block bone graft in the vertical alveolar ridge augmentation. Materials and methods Six adult mongrel dogs were enrolled in this randomized blinded study. After defect preparation, xenogeneic screw-fixed block graft was covered by an occlusive collagen membrane in group 1 that represented the control group (Block + CM). In group 2, L-PRF membrane was added first before top coverage by occlusive collagen membrane (Block + L-PRF + CM). Groups 3 (Block + PCM) and 4 (Block + L-PRF + PCM) were identical to the first two groups except that the occlusive collagen membrane was replaced by a perforated one. Following a healing period of 2 months, the dogs were submitted to the surgical reentry phase for clinical and histological evaluation. Results Clinically, no significant differences were found among all groups regarding vertical and horizontal ridge dimensions (p = 0.155, 0.492, respectively). Histomorphometric analysis revealed that the percentage of the total bone area and mature bone was significantly higher in group 4 (69.36 ± 2.72, 33.11 ± 5.18) compared to the control group (59.17 ± 4.27, 21.94 ± 2.86) (p = 0. 027, p = 0.029). Conclusion The use of xenogenic block grafts in combination with a double-layered perforated collagen L-PRF membrane in vertical ridge augmentation appeared to improve the inductive power of this challenging defect type. Clinical relevance Size and number of perforations may affect the mechanical and handling properties of the membrane.
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