BackgroundOdontogenic cysts are those which arise from the epithelium associated with the development of teeth. Some odontogenic cysts were found to have special biological features that make them distinct from other lesions. This study was conducted to detect the immunoepxression of laminin-1 and Ki-67 in both radicular cysts (RCs) and keratocystic odontogenic tumors (KCOTs) and to examine the possible predictive value of these markers.MethodsThirteen cases of RCs and twelve cases of KCOTs were included in this study. Antibodies against laminin-1 and Ki-67 were used as primary antibodies.Resultsten cases out of thirteen cases of RCs were immunopositive to laminin-1. The immunonegative cases of RCs showed high degree of inflammation inside the connective tissue wall. One case out of twelve cases of KCOTs was immunopositive to laminin-1 and the rest were immunonegative. Seven cases out of thirteen cases of RCs showed immunopositivity for Ki-67 with increased numbers of immunopositive cells when the inflammation was severe in the connective tissue wall. All KCOTS were immunopositive to Ki-67.ConclusionsThe benign nature of radicular cysts and the aggressive behavior of keratocystic odontogenic tumors could be explained by the expression of laminin and Ki-67. Laminin-1 and Ki-67 could be valuable markers for the prediction of the biologic behavior of cystic lesions.

BackgroundThe pathogenesis of salivary gland carcinomas is very complex and prognostic markers are difficult to find in these carcinomas of which the different subtypes have varying malignant potential. The study was conducted to examine the cellular distribution of maspin and MCM2 in salivary gland carcinomas and their value to predict lymph node metastasis.Materials and methodsFifty three paraffin blocks of different lesions (15 muco-epidermoid carcinoma, 14 adenoid cystic carcinoma, 3 epi-myoepithelial carcinoma, 5 salivary duct carcinoma, 5 malignant pleomorphic adenoma, 6 polymorphous low grade adenocarcinoma and 5 acinic cell carcinoma) were prepared for immunohistochemical staining with maspin and MCM2 antibodies. ANOVA and Pearson correlation tests were used for the statistical analysis of the results.ResultsAll salivary gland carcinomas express maspin and MCM2 with variable cellular localization. There was a significant difference in the expression of each antibody between mucoepidermoid carcinoma, adenoid cystic carcinoma and polymorphous low grade adenocarcinoma. No association was found between examined markers and lymph node metastasis.ConclusionsSalivary gland carcinomas express maspin and MCM2 with variable levels and cellular localization, consisting important markers of biological behavior in these tumors. The level of MCM2 expression can be used in the differential diagnosis of adenoid cystic carcinoma and polymorphous low grade adenocarcinoma. Further study with large sample size is recommended to assess their value in prediction of lymph node metastasis.

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Oral squamous cell carcinoma is one of the most common oral cancers, traditional treatments of cancer (surgery, chemotherapy and radiotherapy) have shown to have many side effects and this led to shifting to natural alternatives. Phytochemicals are now showing more appealing future in cancer treatment. Objectives:To study the effect of Ethanolic Neem leaf extract (ENLE) on oral squamous cell carcinoma (OSCC) cell line cells.Material and methods: Immunocytochemical staining of oral squamous cell carcinoma cell line treated with three different concentrations of ENLE for different durations was performed and the levels of caspase-3 and Ki67 were evaluated, followed by statistical analysis.Results: MTT Cytotoxicity Assay showed a progressive decrease in the mean viability percentage in ENLE treated SCC-15 cells as the ENLE concentration increased at both durations. A statistically significant increase was found on investigation of the cytotoxic effect of different concentrations of ENLE at different durations. Caspase-3 results showed an increase in the number of apoptotic cells, with increasing the concentration and duration, when compared with control groups in both durations. Ki-67 results showed a decrease in the number of viable cells, with increasing the concentration and duration, when compared to control groups in both durations. Conclusion:The assessment of cytotoxicity of ENLE on SCC-15 cell line showed that the ENLE had a cytotoxic effect on the cells when compared to the control cells. This effect increased with increasing both the concentration and duration. Immunocytochemistry (ICC) is an effective sensitive marker for both apoptosis and proliferation.