Immunohistochemical detection of laminin-1 and Ki-67 in radicular cysts and keratocystic odontogenic tumors

Abstract

BackgroundOdontogenic cysts are those which arise from the epithelium associated with the development of teeth. Some odontogenic cysts were found to have special biological features that make them distinct from other lesions. This study was conducted to detect the immunoepxression of laminin-1 and Ki-67 in both radicular cysts (RCs) and keratocystic odontogenic tumors (KCOTs) and to examine the possible predictive value of these markers.MethodsThirteen cases of RCs and twelve cases of KCOTs were included in this study. Antibodies against laminin-1 and Ki-67 were used as primary antibodies.Resultsten cases out of thirteen cases of RCs were immunopositive to laminin-1. The immunonegative cases of RCs showed high degree of inflammation inside the connective tissue wall. One case out of twelve cases of KCOTs was immunopositive to laminin-1 and the rest were immunonegative. Seven cases out of thirteen cases of RCs showed immunopositivity for Ki-67 with increased numbers of immunopositive cells when the inflammation was severe in the connective tissue wall. All KCOTS were immunopositive to Ki-67.ConclusionsThe benign nature of radicular cysts and the aggressive behavior of keratocystic odontogenic tumors could be explained by the expression of laminin and Ki-67. Laminin-1 and Ki-67 could be valuable markers for the prediction of the biologic behavior of cystic lesions.

Expressions of bax, bcl-2 and ki-67 in odontogenic keratocysts (keratocystic odontogenic tumor) in comparison with ameloblastomas and radicular cysts

“…Particularly, Ki-67 positive cells were mostly detected in the suprabasal layers of lining epithelium. These results are consistent with many studies (8,9). Kuroyanagi et al reported that high expression of Ki-67 in the basal layer of lining epithelium was seen in recurrent odontogenic keratocysts group (10).…”

Comparative immunoexpression of ICAM-1, TGF-β1 and ki-67 in periapical and residual cysts

“…The ki-67 antigen is a nuclear protein expressed in proliferating cells and absent in quiescent cells (26). There are evidences that the expression of ki-67 on the epithelium lining of odontogenic cysts, including PC, can be elevated in the presence of a more intense inflammatory infiltrate (27), suggesting that an increasing in the proliferative activity on these cysts could be directly influenced by inflammation. Additionally, ki-67 expression seems to be elevated in hyperplastic epithelium in contrast with atrophic epithelium in PC (12,28).…”

Exploring the concept of inflammatory angiogenesis in keratocystic odontogenic tumor

“…Therefore the higher number of these cells would be expectable. Another contributing factor could be the difference in inflammatory cell types between these two lesions, which is composed mainly of lymphocytes in KCOTs compared to a more mixed population in RCs (24,25). Neutrophils and macrophages found in RCs are potent inducers of angiogenesis (6,24), which along with the other inflammatory cell types found in this lesion may have a stronger effect in producing new vasculature in comparison to the mere lymphocytic population of inflamed KCOTs.…”