Exploring the concept of inflammatory angiogenesis in keratocystic odontogenic tumor

Abstract

Objective: The aim of the present study was to investigate the role of inflammation in angiogenesis of keratocystic odontogenic tumor (KCOT). Study Design: Twenty inflamed and 20 non-inflamed KCOTs were selected based on quantitative scoring of inflammation which was also applied on 20 radicular cysts. Microvessel density was assessed in all samples using CD34 antibody and angiogenesis was compared between the three groups. Statistical analysis was performed using one-way analysis of variance followed by post-hoc Scheffe test and P values less than 0.05 were considered significant. Results: A statistically significant difference in angiogenesis was found between radicular cysts and both inflamed and non-inflamed KCOTs (P < 0.001), but not between inflamed and non-inflamed KCOTs (P =0.347). Conclusion: Based on the results obtained in the present study, it seems that the effect of inflammation on angiogenesis in KCOT is minimal. However further investigation using other methods of evaluation is suggested to fully clarify the role of “inflammatory angiogenesis” in this neoplasm. Key words:Keratocystic odontogenic tumor, radicular cyst, angiogenesis, inflammation.

Histopathological and immunohistochemical study in keratocystic odontogenic tumors: Predictive factors of recurrence

“…From these studies, angiogenesis in the 200 µm region immediately below the epithelium was investigated in the present study, and it was identified that the number of blood vessels positive for CD34 expression was associated with the rate of tumor recurrence. Previous studies have reported that CD34 expression is a histopathological marker of tumor aggressiveness ( 4 , 9 , 20 ); however, to the best of our knowledge, the present study is the first to demonstrate that angiogenesis directly beneath the epithelium is a significant factor in predicting the recurrence of KCOTs.…”

Oral tumors in children: Diagnosis and management

“…It has been showed that increasing of reactive oxygen and nitrogen species could also induce expression of a variety of cancer associated gens including the tumor necrosis factor gene (TNF), matrix metalloproteinases (MMP) genes, and vascular endothelial growth factor (VEGF) genes. Up regulation of these genes could affect on cell migration and angiogenesis via regulation of Nuclear Factor ĸB (NFĸB) …”

“…Chronic inflammation is other key pathogenesis event which could induce cancerous conditions via increasing of production of reactive oxygen and nitrogen species . The increasing of reactive oxygen and nitrogen species could lead to increasing of DNA damage which is known as one of key steps in initiation of various cancers such as oral tumors .…”

E‐cadherin regulators are differentially expressed in the epithelium and stroma of keratocystic odontogenic tumors

“…Inflammation causes structural tissue changes and functional alterations in KOTs, mainly resulting from the effect of inflammatory cells on epithelial proliferative activity and cytokeratin expression and with a smaller impact on stromal vascularization . Inflammation significantly affected the immunoreactivity of the study proteins in most RCs.…”

“…The reasons for these different patterns of expression between Snail and Slug are not clear, although new evidence suggests that these transcription repressors may operate either alone or together (35,36). Inflammation causes structural tissue changes (37) and functional alterations in KOTs, mainly resulting from the effect of inflammatory cells on epithelial proliferative activity (38)(39)(40) and cytokeratin expression (41) and with a smaller impact on stromal vascularization (37). Inflammation significantly affected the immunoreactivity of the study proteins in most RCs.…”