The proliferation potential of the epithelium and the overexpression of various anti-apoptotic proteins in odontogenic epithelial tumors are quite significant for their clinical behaviour. High expressions of bcl-2 and Ki-67 in odontogenic keratocysts accord with their aggressive clinical behaviour and a high recurrence rate.
Stronger p63 gene immunoexpression in the group with completely impacted teeth might be a consequence of bigger number of stem cells than it is in the case of the group with partially impacted teeth. This study also supports prophylactic removal of impacted teeth because of the development of pathologies associated with them.
Heat shock proteins (HSPs) are a class of functionally related proteins, the expression of which is increased when cells are exposed to elevated temperatures or other stresses, including infection, irradiation, heavy metals, ethanol, and oxidants. The aim of this study was to investigate the significance of HSP60, HSP70 and HSP27 as prognostic factors in different stages of oral carcinogenesis by immunohistochemical analysis. METHODS Twenty specimens of oral squamous cell carcinoma (OSCC) of the oral cavity were immunostained for HSPs to expose differences in stainability among normal epithelium (n=20) and leukoplakia (n=20) as dysplasia. RESULTS Immunohistochemistry demonstrated that OSCC cells were positive for all of HSP60, HSP70 and HSP27. Leukoplakia cases were positive for HSP70 and HSP27, but stained with variability for HSP60. Normal epithelium expressed HSP60 and to a lesser extent HSP70, while HSP27 were hardly ever expressed. The HSP70 and HSP27 stainings in OSCC were significantly higher than in normal epithelium, and demonstrated almost the same staining character as with leukoplakia. CONCLUSION Our results demonstrated that HSP immunochemistry revealed changes in especially HSP70 and HSP27 expression during tumorigenesis of squamous epithelium of the oral cavity.
Purpose: Ki-67 and COX-2 are two markers which are commonly used to assess tumor proliferation. The aims of this study were to evaluate and compare the presence of Ki-67 and COX-2 in odontogenic keratocyst (OKC), radicular cyst (RC), ameloblastoma (AML) and dental follicle (DF) in order to determine proliferative potentials of such lesions.
Materials and Methods:This study has been conducted on 80 samples obtained from non-smoking, healthy subjects diagnosed as having either OKC, RC, AML or DF. 20 samples obtained from healthy mucosa served as controls. Slides prepared from paraffin-embedded sections were immunohistochemically stained for Ki-67 and COX-2 expressions.Results: Ki-67 and COX-2 expressions in the OKC and AML groups were found to be significantly higher than those observed in the RC, DF and healthy mucosa groups (p < 0.005 for each).Conclusion: High levels of Ki-67 and COX-2 staining in OKC and AML can be considered as the indicators of high proliferation tendency and mitotic activity. These markers could provide valuable tools for the treatment process when they are available for widespread use to detect cases that have significant relapse potential and aggressive behavior.
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