Medina. Approximately forty patients a day were Received for publication January 5, 1971 examined for 3 days. At the suggestion of the epidemiologist, the patients were numbered from 150 to 272, so that the memorizing of numbers would be reduced to a minimum. The studies performed on each patient are listed below.

The need for a consistently reliable silver stain for Treponema pallidum in paraffin sections made us aware that further investigation of the technique was needed. Three methods were tried and discarded:(1) The Levaditi stain, which was originally planned for use in staining entire blocks of tissue fixed in 10 per cent. formalin. After the staining is completed, the stained tissue block is embedded in paraffin and sectioned. This method was found to be extremely time-consuming; 3 to 5 days were required for the silver nitrate impregnation alone, and another 27 to 72 hours for the silver reduction.Numerous attempts were made to adapt this technique for use with single paraffin sections without success.(2) The Steiner and Steiner (1944) stain for spirochaetes and Donovan bodies gave some positive results with control material. However, it was timeconsuming, and the results obtained were not consistent.(3) The Dieterle stain for spirochaetes produced only fair results. The staining procedure was quite lengthy and this precluded its use in a laboratory performing a large number of silver stains.

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