A new modification of the Krajian silver stain for Treponema pallidum.

Abstract

The need for a consistently reliable silver stain for Treponema pallidum in paraffin sections made us aware that further investigation of the technique was needed. Three methods were tried and discarded:(1) The Levaditi stain, which was originally planned for use in staining entire blocks of tissue fixed in 10 per cent. formalin. After the staining is completed, the stained tissue block is embedded in paraffin and sectioned. This method was found to be extremely time-consuming; 3 to 5 days were required for the silver nitrate impregnation alone, and another 27 to 72 hours for the silver reduction.Numerous attempts were made to adapt this technique for use with single paraffin sections without success.(2) The Steiner and Steiner (1944) stain for spirochaetes and Donovan bodies gave some positive results with control material. However, it was timeconsuming, and the results obtained were not consistent.(3) The Dieterle stain for spirochaetes produced only fair results. The staining procedure was quite lengthy and this precluded its use in a laboratory performing a large number of silver stains.

Keratopustular variety of framboesiform syphilis: a case report.

“…Histopathology A small papule from the infrascapular region was excised and then divided into two parts: one for routine histopathological procedures, the other for silver impregnation using a modified Krajian's method (Walter et al, 1969).…”

Syphilitic balanitis of Follmann developing after the appearance of the primary chancre. A case report.

“…HISTOPATHOLOGY A biopsy of the flat non-ulcerated lesions on the glans was performed and the sections stained with haematoxylin and eosin (HE) as well as with the modification of the Krajian silver stain (Walter, Smith, Israel, and Gager, 1969).…”

Early malignant syphilis observed during infection and reinfection in the same patient.

“…The WR and VDRL results were I ,i inegative; the TPI result was positive with 100% immobilisation; and the FTAand the hip were taken. Sections from both specimens were stained with haematoxylin and eosin and with modification of the Krajian silver impregnation method(Walter et al, 1969).…”