Pemberian Air Susu Ibu (ASI) akan berakhir saat bayi berusia 6 bulan. Pada usia selanjutnya sampai dengan 24 bulan bayi memerlukan makanan pendamping ASI. MPASI merupakan makanan pelengkap gizi sebagai penopang pertumbuhan dan perkembangan bayi. Daun tanaman kelor (Moringa oleifera, Lam) merupakan bahan makanan padat nilai gizi. Tepung kering daun ini digunakan sebagai komponen pembuatan MPASI yang murah, aman, mudah didapatkan dan bergizi tinggi. Padukuhan Morobangun salah satu padukuhan yang memiliki populasi bayi usia 6-24 bulan yang cukup tinggi, dan 28,8% diantaranya merupakan bayi yang memiliki berat badan minimal, dikhawatirkan akan memunculkan balita stunting di kemudian hari. Pengabdian Masyarakat dilaksanakan di Padukuhan Morobangun, Jogotirto, Berbah, Sleman, Yogyakarta, pada tanggal 12-13 Oktober 2020, dengan sasaran ibu-ibu PKK dan kader kesehatan balita. Metode yang digunakan adalah penyuluhan yang dilaksanakan dengan ceramah baik secara daring maupun luring. Difusi iptek yang digunakan adalah pelatihan dan pendampingan pembuatan MPASI yang berupa baby cookies dengan komponen tepung daun kelor dan puding. Dihasilkan MPASI baby cookies versi gluten, gluten free, semprit dan puding kelor jagung manis. MPASI tepung daun kelor merupakan makanan bayi yang sehat, murah, aman, mudah dibuat, serta kaya gizi dan disukai bayi.

<p><strong><em>Introduction:</em></strong><em> </em><em>Rabies is a disease of animals (usually vertebrate) which is zoonotic (transmissible to humans). Rabies occurs in 24 out of the 34 provinces of Indonesia. Rabies is caused by a virus in the Family: Rhabdivoridae, Genus: Lyssavirus. Control the dog population and dog vaccination is effective and economical method to prevent the incidences of rabies in humans. This study aims to investigate the relationship between level of knowledge and attitude to the practice in prevention rabies infection in residents lived in area covered by Puskesmas Kuta II.</em><em></em></p><p><strong><em>Methods:</em></strong><em> </em><em>This study was a cross-sectional study and carried out by simple random sampling of 96 residents in the area of Puskesmas Kuta II. In this study, the independent variables are knowledge of and attitudes towards rabies disease while the dependent variable is the </em><em>practice </em><em>to prevent </em><em>rabies infection in human. Data were collected by a questionnaire-based interview. Analyses were then carried out using the chi-square (x²) followed by multivariate analysis using multiple logistic regressions.</em></p><p><strong><em>Results:</em></strong><em> </em><em>This study showed that knowledge has a significant relationship with </em><em>practice</em><em> for rabies disease prevention (p = 0.013; OR 4.240; 95% CI 1.352 up to 13.296). Attitude has a significant positive relationship with the value of p</em><em>ractice </em><em>for rabies disease prevention (p = 0.015; OR 3.073; 95% CI 1.233 up to 7.627). The combined effect of knowledge and attitudes to pr</em><em>actice </em><em>for rabies prevention were approximately 20.90% (Negerkerke R square = 0.209).</em></p><p><strong><em>Conclusions:</em></strong><em> </em><em>There was a significant relationship between knowledge and attitudes with the </em><em>practice </em><em>for rabies disease prevention in residents in the area of Puskesmas Kuta II, the higher level of knowledge and improved attitudes will improve </em><em>practice for</em><em> rabies disease prevention amongst residents in the area of Puskesmas Kuta II.</em></p><p><em> </em></p><p><strong><em>Key words</em></strong><em>: </em><em>Rabies;  Rabies;  Preventio; Knowledge; Attitude;  Practice</em><em></em></p>

Pengaruh pemberian fraksi etanolik dan petroleum eter ekstrak umbi bawang dayak (Eleutherine palmifolia) terhadap ekspresi p53 mutan pada galur sel kanker payudara T47D Breast cancer was still to be the most popular disease. The second highest morbidity and mortality stages after cervix cancer which need to be involved in alternative therapy. Bawang dayak (Eleutherine palmifolia (L.) Merr ) had been used as anti-cancer in empiric therapy by urban society. Therefore, it was needed to determine the influence of etanolic and petroleum eter fractions of bawang dayak extract on the p53 mutant expression in T47D breast cancer cell in vitro. Epithelial cell in ductal mammae breast cancer T47D which had a malignancy and p53 mutant, with ER/PR positive status. As a comparation, it was used MCF7 cell specimen (negative control). They were cultured on 60 wells for T47D and MCF7 in RPMI 1640 media. Each well was filled with 2x10 5 cells/200 µl media. Thirty wells with a size of 1.5 cm/T47D cell diameter, cultured in etanolic and petroleum eter fractions with under concentration of LC50. The number of sample was determined by using "Rule of Thumb". The result of LC50 on T47D breast cancer cell were etanolic fraction 125 μg/mL, while petroleum eter fraction 31.25 μg/mL. The result of expression percentage of p53 mutant given by the extract of etanolic fraction of bawang dayak were: 0 μg/mL = 36.11%, 15.625 μg/mL = 28.32%, 31.25 μg/mL = 27.46%, 62.5 μg/mL = 19.67%, and 125 μg/mL = 11.02%. Other result of bawang dayak extract percentage given in petroleum eter fraction were: 0 μg/mL = 26.16%, 3.90625 μg/mL = 25.29%, 7.8125 μg/mL = 22.70%, 15.625 μg/mL = 22.27%, and 31.25 μg/mL =15.78%. The treatment of bawang dayak extract in ethanolic and petroleum eter fractions were able to inhibit the expression of p53 mutant in vitro. The result of this research showed no significant difference in the inhibition expression of p53 mutant in breast cancer cell T47D.

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Objective: This study was designed to evaluate the antihypercholesterolemic effect of Elephantosus scaber on histopatology of Sprague-Dawley rat's aorta with hypercholesterolemia diet model. Design and method: This is an Experimental study with post test only control group design. Twenty-one (21) Sprague-Dawley rats of about 2–3 months old, weighing between 150–200 g body weight, were used for the study with incidental sampling. The rats were divided into three groups (3) of seven animals in each cage. First group (G1) was the normal control administered standard meal. Positive control group (G2) and Treatment group (G3) were administered with high fat diet. Administration of 400 mg/kg body weight of Elephantopus scaber extract was given to the third group in 7–11 weeks after induced by high fat diet. Termination was done after last feed administration. Aorta section was done with Hematoxylin eosin stain. Data analysis used Kruskal-wallis followed by Mann-Whitney test. Results: Proximate measurement was carried out on the Sprague-Dawley rat's aorta section with the following results: wall thickness in normal control group 85.19 ± 8.35 mm; positive control group 95.78 ± 17.64 mm and treatment group 80.50 ± 8.41 mm. Data analysis of Kruskal-Wallis was obtained P = 0.018, a > P. The results obtained from the analysis of Mann-Whitney test are significant difference between normal control group (G1) and positive control group (G2). And non-significant difference between normal control group (G1) and treatment group (G3). Conclusions: Elephantosus scaber extract has antihypercholesterolemic potential effect that could reduce atherosclerosis on histopatology of aorta section in Sprague-Dawley rats with hypercholesterolemia diet model.

Colon cancer is a malignancy in gastrointestinal tract. It causes high mortality rate in global cancer population. However, chemotherapy as its first option therapy is still controversial due to its effectiveness and its adverse effects. Finding supportive and alternative drugs to cure cancer is one of focus in cancer research. A drug which also has anticancer effects is metformin. Metformin is a biguanide antidiabetic which show its potential anticancer benefit in metabolic-related cancers including colon cancer. To investigate anticancer potency of metformin in targeting p53-related molecules. Metformin treatment were divided into 4 groups by 0, 5, 10 and 20 mM concentrations and incubated in 37°C and 5% CO2 condition for 48 hours. Immunohistochemistry were conducted to asses level of expression of Bax, p21, cyclin D1 and E2F1, respectively. Level of expression were measured by H-SCORE using percentage and intensity calculation. Comparisons of H-SCORE between groups were performed by ANOVA for parametrical data and Kruskal-Wallis for nonparametrical data. Growth inhibition were observed after metformin treatment. Metformin increases Bax expression significantly at all concentrations. p21 expression was also increased after metformin treatment but is not statistically significant. Subsequently, metformin decreases cyclin D1 expression at 10 and 20 mM concentration thus decreased E2F1 expression at 5 and 10 mM concentration. These data suggest that metformin may have potential therapeutic effects in mutant p53 colon cancer cell line by targeting p53-related molecules.