Young leaflets were used as the explants for in vitro regeneration of Albizia falcataria (L.) Fosberg without callus intervention. The leaf explants produced in vitro adventitious shoot buds directly on culturing with MS supplemented with BA, IBA (4.0/0.05 mg/l) and 10% coconut milk (v/v). Addition of casein hydrolysate and coconut milk increased the production of shoot buds. The buds produced shoots and roots and showed 66% survival in a field trial. This technique offers an effective way by which large number of genetically stable plants can be produced, maintained, multiplied and transported as disease free propagules or regenerants, safely and economically.
Shoot apices of 7-day old foxtail millet(Setaria italica(L) Beauv.) seedlings treated with 2 and 20 mg/L of dimethyltetrachloroterephthalate (DCPA) were examined under the electron microscope. Cell division is interrupted. The nucleus and nucleolus do not disintegrate and chromosomes do not differentiate. Instead, giant nuclei and giant nucleoli occupy most of the cell volume in the meristematic regions. Several nucleolar caps form on the giant nucleolus; and in the advanced stages, they separate and are encircled by a nuclear membrane to form multiple nuclei. Other organelles are also affected. Cristae and thylakoid membranes of mitochondria and chloroplasts degenerate and multiple vacuoles form. Cell walls are markedly more osmophilic after DCPA treatment. Treated cells are less turgid than controls.
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