Rapid in vitro Regeneration and Clonal Propagation of the Fastest Growing Leguminous Tree Albizia falcataria (L.) Fosberg using Leaflet Explant

Abstract

Young leaflets were used as the explants for in vitro regeneration of Albizia falcataria (L.) Fosberg without callus intervention. The leaf explants produced in vitro adventitious shoot buds directly on culturing with MS supplemented with BA, IBA (4.0/0.05 mg/l) and 10% coconut milk (v/v). Addition of casein hydrolysate and coconut milk increased the production of shoot buds. The buds produced shoots and roots and showed 66% survival in a field trial. This technique offers an effective way by which large number of genetically stable plants can be produced, maintained, multiplied and transported as disease free propagules or regenerants, safely and economically.

Phytochemical and toxicological analysis ofAlbizia falcatariasawdust

“…The growth in demand from the Asian wood products markets has resulted in increased acreage and production of Albizia , as well as other fast growing hardwoods, within several Southeast Asian nations. While board foot production has increased, efforts in improving the quality of these woods for improved durability, growth rate and insect resistance through amplified fragment length polymorphism based strategies (Aparajita and Rout 2009), optimisation of propagation techniques (Ghosh et al 2010), and investigations to identify physical and chemical properties and quantify their variability within and between Albizia species is actively occurring (El-Harwary et al 2011). These international research programs will likely further promote the inclusion of Albizia sp.…”

In vitro clonal propagation of Achyranthes aspera L. and Achyranthes bidentata Blume using nodal explants

“…In vitro propagation methods offer highly efficient tools for medicinal plants useful for pharmaceutical industry [9] . Plant tissue culture techniques offer a powerful tool for mass multiplication of medicinally important plant species [9][10][11][12][13][14][15][16][17][18][19][20] . There has been progress in tissue culture studies in many Amaranthaceae members such as Alternanthera sessilis, Amaranthus spp, Amaranthus paniculatus [20,21] .…”

An improved protocol for carrot haploid and doubled haploid plant production using induced parthenogenesis and ovule excision in vitro

“…Although callus may develop from haploid cells, more often it results of somatic cell proliferation (Thomas et al 2003 , Staniaszek and Habdas 2006 ) and is undesired in haploid production. Additionally, callus is a genetically unstable tissue in which a high degree of genetic variation can occur (Ghosh et al 2010 ) resulting also in altered ploidy of regenerated plants (Larkin and Scowcroft 1981 ). In this light, the use of pollination without 2,4-D treatment was better for direct production of carrot haploid and DH plants.…”