Abstract. Putra AN, Mustahal, Syamsunarno MB, Hermawan D, Fatimah DG, Putri PB, Sevia, Isnaini R, Herjayanto M. 2021. Dietary Bacillus NP5 supplement impacts on growth, nutrient digestibility, immune response, and resistance to Aeromonas hydrophila infection of African Catfish, Clarias gariepinus. Biodiversitas 22: 253-261. This study aims to investigate the effects of Bacillus NP5 supplementation as a probiotic on growth, immune response, and resistance of African catfish to Aeromonas hydrophila infection. Catfish with an initial weight of 6.8 ± 0.1 g were fed with different doses of Bacillus NP5 (0%, 1.1%, 1.2% B, 1.3%, and 1.4%) with three replications and reared for 60 days. The first 45 days were used to observed growth performance, and the last 15 days were used to perform challenge tests against A. hydrophila infection. The results showed that the treatment of 1.2% Bacillus NP5 results in the highest specific growth rate (2.55±0.28 day-1) and increased protein and lipid digestibility significantly (P<0.05). Treatment of 1.1-1.3% Bacillus NP5 increase amylase and lipase activity that significantly higher than 0% Bacillus NP5 treatment. Supplementation of Bacillus NP5 significantly increased the leukocyte, phagocytic index, and survival rate in African catfish after A. hydrophila infection. Therefore, the supplementation of 1.2% Bacillus NP5 in the feed increased the growth, immune response, and African catfish resistance to the infection.
This study was aimed to evaluate the use of corn steep powder (CSP) as a plant protein source in Oreochromis niloticus diet. A commercial feed with 28% protein content and 368 kcal/g energy was used as reference diet, while the test feed consisting of various CSP content, namely 0%, 10%, 20%, and 30% and feed containing soybean meal (SBM) at the level of 20% and 30%. Tilapia were used in the trial with the initial body weight of 6.44 ± 0.29 g, and reared for thirty days in the aquarium at the density of fifteen and fed 3 times daily at a satiation level. All diets were supplied by 0.5% of Cr2O3 as an indicator for digestibility measurement. This study applied the completely randomized design experimental method containing six diet treatments and four replications. The result showed that CSP contains 40.27% protein, 26.10% lactic acid, and minerals. CSP is low in crude fiber and anti-nutritional factors. This study results that increasing the level of CSP significantly decreased feed acidity (P <0.05) compared to the control. The addition of CSP 20% increased feed digestibility including protein, lipid, energy, and dry matter digestibility. CSP 20% treatment increased final body weight, specific growth rate and reduced feed conversion ratio significantly (P<0.05) compare to other treatments. In conclusion, CSP can be used up to 20% to improve the growth performance of tilapia. Keyword: corn steep powder, feed digestibility, growth performance, tilapia ABSTRAK Penelitian ini bertujuan mengevaluasi penggunaan corn steep powder (CSP) sebagai sumber protein nabati pada pakan ikan nila Oreochromis niloticus. Pakan komersial dengan kadar protein 28% dan energi 368 kkal/g digunakan sebagai pakan acuan, sementara pakan uji terdiri atas pakan dengan kandungan CSP sebanyak 0% (CSP0), 10% (CSP10), 20% (CSP20) dan 30% (CSP30) serta pakan dengan kandungan tepung bungkil kedelai (SBM) pada level 20% (SBM20) dan 30% (SBM30) sebagai pembanding. Penambahan Cr2O3 0,5 % diberikan sebagai indikator untuk mengukur kecernaan. Ikan nila dengan bobot tubuh rata-rata 6.44 ± 0.29 dipelihara dalam akuarium (95×45×45 cm3) yang diisi air 100 L dengan kepadatan 15 ekor per akuarium dan diberi pakan tiga kali sehari secara at satiation selama 30 hari masa pemeliharaan. Penelitian ini menggunakan desain rancangan acak lengkap dengan enam perlakuan dan empat ulangan. Hasil penelitian menunjukkan bahwa CSP mengandung protein sebesar 40,27%, asam laktat 26,10%, beberapa mineral dan indeks asam amino esensial 0,90. CSP juga rendah serat kasar dan zat antinutrisi. Peningkatan dosis CSP menurunkan pH pakan secara signifikan (P<0.05) dibandingkan dengan kontrol. Penambahan CSP sampai level 20% meningkatkan nilai kecernaan total, kecernaan bahan, kecernaan protein, kecernaan lemak dan kecernaan energi. Di samping itu, perlakuan CSP 20% meningkatkan bobot akhir, laju pertumbuhan harian dan rasio konversi pakan yang signifikan (P<0,05) dibandingkan perlakuan lainnya. Hasil penelitian ini menunjukkan bahwa pemberian CSP 20% dapat meningkatkan kinerja pertumbuhan ikan nila. Kata kunci: corn steep powder, kecernaan pakan, pertumbuhan, ikan nila
The vegetable waste silage is potential as a feed ingredient for tilapia culture. Silage is a product of the processing of materials by the addition of acid or by anaerobic fermentation. This study aimed to evaluate the effect of vegetable waste silages on the growth of tilapia (Oreochromis niloticus). Formic acid is used in this study as organic acid for the preservative. Four diets concentration of formic acid in making silage (0%, 3%, 4%, and 5%) was formulated containing 27% crude protein. The result showed that total digestibility, protein digestibility, and fat digestibility were significantly higher (P<0.05) in 4% formic acid and 5% formic acid than Control. The value of growth was significantly highest (P<0.05) in 5% formic acid (2.96±0.08 %/day), followed by 4% formic acid (2,66±0,14 %/day), 3% formic acid (2,56±0,05 %/day) and control (2,54±0,21 %/day). The utilization of 5% formic acid in making vegetable waste silage showed the best result on growth and feed efficiency than the other treatments.
Seaweed has been cultivated with the purpose to meet a demand of market which incrense immediately. One of factors that can determine the success of seaweed culture is the right location to meet requirement for seaweed cultivation. The purpose of this study to analyze physical and chemical parameters in the Lontar village. Determination of station with randomized method that represent the location that includes 5 stations with repetition three times. Measurement of physical and chemical parameters that the brightness of the water, wave height, current velocity, depth, temperature, salinity, pH conducted at research sites. While for nitrate, phosphate and TSS parameter were measured in the analysis laboratory of department of water resources and settlements (DSAP provinsi Banten). Results of testing the physical and chemical parameters in the waters of Lontar village at District Tirtayasa, Serang, in November-December 2014 can be stated suitable to be used as a location for seaweed cultivation Kappaphycus alvarezii. While the test results showed that the suitability of the location of all stations which observed were stated kind and proper to be used as a location for seaweed cultivation Kappaphypus alvarezzi.
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